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Genetic diversity for seed protein composition in Medicago truncatula

Published online by Cambridge University Press:  12 February 2007

C. Le Signor ,
K. Gallardo ,
J.M. Prosperi ,
C. Salon ,
L. Quillien ,
R. Thompson  and
G. Duc
C. Le Signor*
Affiliation:
Unité de recherche en génétique et écophysiologie des légumineuses, BP 86510, F21065Dijon Cedex
K. Gallardo
Affiliation:
Unité de recherche en génétique et écophysiologie des légumineuses, BP 86510, F21065Dijon Cedex
J.M. Prosperi
Affiliation:
UMR-DGPC Station de génétique et d'amélioration des plantes INRA, Domaine de Melgueil, F34130Mauguio
C. Salon
Affiliation:
Unité de recherche en génétique et écophysiologie des légumineuses, BP 86510, F21065Dijon Cedex
L. Quillien
Affiliation:
Unité de biochimie et technologie des protéines, INRA, F44316 Nantes Cedex 03, France
R. Thompson
Affiliation:
Unité de recherche en génétique et écophysiologie des légumineuses, BP 86510, F21065Dijon Cedex
G. Duc
Affiliation:
Unité de recherche en génétique et écophysiologie des légumineuses, BP 86510, F21065Dijon Cedex
*
*Corresponding author: E-mail:, lesignor@epoisses.inra.fr
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Abstract

Fifty lines of Medicago truncatula, derived from ecotypes or cultivars of diverse geographical origin, were grown under uniform conditions, and variation in seed protein composition and quantity was investigated. One-dimensional electrophoretic profiles revealed 46 major seed polypeptides, of which 26 were polymorphic within the collection. The vicilin/convicilin (7S) and the legumin (11S) type proteins were identified by immunoblotting using antibodies raised against the homologous pea proteins. The polymorphism for the major seed protein classes allowed the clustering of the genotypes into four groups. There was no evidence of clustering according to geographical origin of the lines. However, all lines not belonging to either M. truncatula ssp. truncatula or ssp. longispina were clustered in a single group, demonstrating the value of seed protein profiles in delimiting species boundaries. The Jemalong line group was differentiated early in the dendrogram, and thus represents an ancient clade in the seed diversity of M. truncatula. Within-accession variation was investigated for one-dimensional seed profiles, with additional lines obtained from the same ecotypes. As expected for an autogamous species, within-accession variation was low. Seed protein content was highly variable among the 50 lines examined. Lines contrasting for qualitative traits and seed protein content were identified to allow for the genetic determination of these characters.

Keywords

ecotypeelectrophoresisgenetic variationimmunoblottingMedicago truncatulaseed protein content
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 1 , April 2005 , pp. 59 - 71
Copyright
Copyright © NIAB 2005

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