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Cloning, sequencing and oocyte-specific expression of the marmoset sperm receptor protein, ZP3

Published online by Cambridge University Press:  26 September 2008

P Thillai-Koothan ,
Marcel van Duin  and
R. John Aitken
P Thillai-Koothan
Affiliation:
MRC Reproductive Biology Unit, Edinburgh, UK, and Organon International, Oss, The Netherlands
Marcel van Duin
Affiliation:
MRC Reproductive Biology Unit, Edinburgh, UK, and Organon International, Oss, The Netherlands
R. John Aitken*
Affiliation:
MRC Reproductive Biology Unit, Edinburgh, UK, and Organon International, Oss, The Netherlands
*
Professor R.J. Aitken, MRC Reproductive Biology Unit, 37 Chalmers Street, Edinburgh EH3 9EW, Scotland, UK.
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Summary

The zona pellucida surrounding the mammalian oocyte contains a major glycoprotein species, ZP3, that serves as a cell- and species-specific receptor for spermatozoa. In this study we have determined the primary amino acid structure of marmoset ZP3 (marZP3) and examined the expression of marZP3 mRNA within the ovary. The marZP3 gene possesses an open reading frame of 1272 nucleotides which is expressed specifically by the oocyte and encodes a polypeptide chain of 424 amino acids that exhibits 91% homology with the human ZP3 sequence. The disparity between these molecules was confined to a short domain spanning residues 322–352; otherwise the molecules were very similar, showing conservation of many structural features including the N–linked glycosylation sites, location and number of cysteine and proline residues and hydrophobicity profile. The results of this study have important implications for the use of the marmoset monkey as an animal model for the development of contraceptive vaccines targeting ZP3.

Keywords

MarmosetSperm receptorZona pellucidaZP3
Type
Article
Information
Zygote , Volume 1 , Issue 2 , May 1993 , pp. 93 - 101
Copyright
Copyright © Cambridge University Press 1993

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References

Angerer, L.M. & Angerer, R.C. (1981). Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization. Nucleic Acids Res. 9, 2819–40.CrossRefGoogle ScholarPubMed
Bleil, J.D. & Wassarman, P.M. (1980). Structure and function of the zona pellucida: identification and characterization of the proteins of the mouse oocyte's zona pellucida. Dev. Biol. 76, 185–202.CrossRefGoogle ScholarPubMed
Chamberlin, M.E. & Dean, J. (1989). Genomic organization of a sex specific gene: the primary sperm receptor of the mouse zona pellucida. Dev. Biol. 131, 207–14.CrossRefGoogle ScholarPubMed
Chamberlin, M.E. & Dean, J. (1990). Human homolog of the mouse sperm receptor. Proc. Natl. Acad. Sci. USA 87, 6014–18.CrossRefGoogle ScholarPubMed
Kinloch, R.A. & Wassarman, P.M. (1989). Nucleotide sequence of the gene encoding zona pellucida glycoprotein ZP3 – the mouse sperm receptor. Nucleic Acids Res. 17, 2861–3.CrossRefGoogle ScholarPubMed
Kinloch, R.A., Roller, R.J., Fimiani, C.M., Wassarman, D.A. & Wassarman, P.M. (1988). Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning. Proc. Natl. Acad. Sci. USA 85, 6409–13.CrossRefGoogle ScholarPubMed
Kinloch, R.A., Ruiz, Seiler B. & Wassarman, P.M. (1990). Genomic organization and polypeptide primary structure of zona pellucida glycoprotein hZP3, the hamster sperm receptor. Dev. Biol. 142, 414–21.CrossRefGoogle ScholarPubMed
Kornfeld, R. & Kornfeld, S. (1985). Assembly of asparagine-linked oligosaccharides. Annu. Rev. Biochem. 54, 631–64.CrossRefGoogle ScholarPubMed
Kyte, J. & Doolittle, R.F. (1982). A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105–32.CrossRefGoogle ScholarPubMed
Lira, S.A., Kinloch, R.A., Mortillo, S. & Wassarman, P.M. (1990). An upstream region of the mouse ZP3 gene directs expression of firefly luciferase specifically to growing oocytes in transgenic mice. Proc. Natl. Acad. Sci. USA 87, 7215–19.CrossRefGoogle ScholarPubMed
Moller, C.C., Bleil, J.D., Kinloch, R.A. & Wassarman, P.M. (1990). Structural and functional relationships between mouse and hamster zona pellucida glycoproteins. Dev. Biol. 137, 2276–86.CrossRefGoogle ScholarPubMed
Paterson, M., Koothan, P.T., Morris, K.D., O'Bryne, K.T., Braude, P., Williams, A. & Aitken, R.J. (1992). Analysis of the contraceptive potential of antibodies against native and deglycosylated porcine ZP3 in vivo and in vitro. Biol. Reprod. 46, 523–34.CrossRefGoogle ScholarPubMed
Philpott, C.C., Ringuette, M.J. & Dean, J. (1987). Oocyte-specific expression and developmental regulation of ZP3, the sperm receptor of the mouse zona pellucida. Dev. Biol. 121, 568–75.CrossRefGoogle ScholarPubMed
Ringuette, M.J., Chamberlin, M.E., Baur, A.W., Sobieski, D.A. & Dean, J. (1988). Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Dev. Biol. 127, 287–95.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. & Maniaris, T. (1989). Molecular Cloning. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
Sanger, F., Nicklen, S. & Coulson, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74, 5463–7.CrossRefGoogle ScholarPubMed
Shabanowitz, R.B. (1990). Mouse antibodies to human zona pellucida: evidence that human ZP3 is strongly immunogenic and contains two distinct isomer chains. Biol. Reprod. 43, 260–70.CrossRefGoogle ScholarPubMed
Struck, D.K. & Lennarz, W.J. (1980). The function of saccharide-lipids in synthesis of glycoproteins. In The Biochemistry of Glycoproteins and Proteoglycans, ed. W.J., Lennarz, pp. 35–84. New York: Plenum Press.CrossRefGoogle Scholar
Van Duin, M., Polman, J., Verkoelen, C., Bunschoten, H., Meyerink, J., Olijve, W. & Aitken, R.J. (1992). Cloning and characterization of the human sperm receptor ligand ZP3: evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations. Genomics 14 (in press).CrossRefGoogle ScholarPubMed
Van prooijen-Knegt, A.C., Rapp, A.K., Van der Burg, M.J.M., Vrolijk, J. & Van de Ploeg, M. (1982). Spreading and staining human metaphase chromosomes on aminoalkyl-silane treated glass slides. Histochem. J. 14, 333–4.CrossRefGoogle ScholarPubMed
Von Heijne, G. (1986). A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683–90.CrossRefGoogle ScholarPubMed
Wassarman, P.M. (1988). Zona pellucida glycoproteins. Annu. Rev. Biochem. 57, 415–42.CrossRefGoogle ScholarPubMed
Wassarman, P.M. (1990 a). Profile of a mammalian sperm receptor. Development 108, 1–17.CrossRefGoogle ScholarPubMed
Wassarman, P.M. (1990 b). Regulation of mammalian fertilization by zona pellucida glycoproteins. j. Reprod. Fertil. (Suppl.) 42, 79–87.Google ScholarPubMed
Winship, P.R. (1989). An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Res. 17, 1266.CrossRefGoogle ScholarPubMed