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Raman Spectroscopic Evidence for Side-Chain Unfolding in Spider Dragline Silk under Tensile Deformation

Published online by Cambridge University Press:  01 February 2011

Xiaojun He ,
Michael S. Ellison  and
Jacqueline M. Palmer
Xiaojun He
Affiliation:
School of Materials Science & Engineering, 161 Sirrine Hall
Michael S. Ellison
Affiliation:
School of Materials Science & Engineering, 161 Sirrine Hall
Jacqueline M. Palmer
Affiliation:
Department of Genetics, Biochemistry & Life Science Studies; Clemson University, Clemson, South Carolina 29634-0971
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Abstract

In-situ Ramanspectra were collected on the N. clavipes spider dragline silk under a tensile deformation rate of 15mm/min. The most prominent features on the spectra were due to those bands near 1100 cm-1, which present as a sensitive probe to structural changes associated with side-chains of silk peptide. A downshift of Raman bands at 1095 cm-1 and 1089 cm-1 was detected with increasing strain. Furthermore, an increase in the intensity of the Raman band at 1062 cm-1 due to the vibration of trans structure without lateral coupling was prominent at certain strain levels. This was interpreted in terms of a morphology transition from the random configuration to the trans conformation modulated by the reorganization of the hydrogen bonding among the side-chain.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 874: Symposium l – Structure and Mechanical Behavior of Biological Materials , 2005 , L4.6
Copyright
Copyright © Materials Research Society 2005

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