Hostname: page-component-7c8c6479df-5xszh Total loading time: 0 Render date: 2024-03-18T02:18:20.554Z Has data issue: false hasContentIssue false

Construction and analysis of an AFLP molecular linkage map of the silkworm (Bombyx mori)

Published online by Cambridge University Press:  12 February 2007

Sima Yang-Hu
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China College of Life Science, Soochow University, Suzhou 215006, China
Li Bin
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
Chen Da-Xia
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
Sun De-Bin
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
Zhao Ai-Chun
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
Zhang Lie
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
Lu Cheng*
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
He Si-Mei
Affiliation:
Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China
Xiang Zhong-Huai
Affiliation:
Key Laboratory of Sericulture, Ministry of Agriculture, College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China
*
*Corresponding author: E-mail: lucheng@swau.cq.cn

Abstract

An amplification fragment length polymorphism (AFLP) molecular linkage map with a relatively high density for location of quantitative trait loci (QTLs) controlling the quantitative traits of silkworm (Bombyx mori) cocoons, was constructed using 91 individuals of the F2 generation. Among the 692 effective loci, 550 were allocated to subgroups a and b, of which 21 linkage groups in subgroup a had 233 molecular markers and 28 linkage groups in subgroup b had 317 markers. The number of markers on each linkage group in subgroups a and b ranged from 4 to 43 and 3 to 35, respectively. The total length of linkage groups for subgroup a was 1868.10 cM, and 2677.50 cM for subgroup b. The length of linkage group varied from 22.3 to 424.3 cM in subgroup a and from 2.4 to 366.5 cM in subgroup b. The average variation in the distance between markers was 3.39–17.43 cM in subgroup a and 0.8–26.96 cM in subgroup b. The average distance between the markers was 8.81 cM in subgroup a and 9.26 cM in subgroup b. There were 14 linkage groups, with an average distance below 10 cM, in subgroup a and 18 linkage groups in subgroup b. There were seven linkage groups with an average distance between 10 and 20 cM in subgroup a and ten linkage groups in subgroup b. Each linkage group in subgroup a had 11.1 loci on average, while there were 11.31 loci on each linkage group on average in subgroup b. The mean length for linkage groups in subgroups a and b was 89 and 95.6 cM, respectively. The total average length for both a and b subgroups was 2272.8, and 9.06 cM for the average marker distance, fulfilling the basic known requirements for locating QTLs.

Type
Research Article
Copyright
Copyright © China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Goldsmith, MR (1991) The Bombyx mori genome mapping project. Sericolosia 31: 145155.Google Scholar
He, NJ, Lu, C, Li, B, Zhou, ZY and Xiang, ZH (2001) The construction of linkage map of Bombyx mori by combination of SADF and RAPD. Acta Entomologia Sinica 44 (4): 476482 (in Chinese with English abstract).Google Scholar
Lander, ES, Green, P and Abrahamson, J (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural population. Genomics 1: 174181.Google Scholar
Li, B, Lu, C, Zhou, ZY and Xiang, ZH (2000) The construction of RAPD linkage map of Bombyx mori. Acta Genetica Sinica 27 (2): 127132 (in Chinese with English abstract).Google Scholar
Promboon, A, Shimada, T, Fujiwara, H and Kobayashi, M (1995) Linkage map of RAPDs in the silkworm, Bombyx mori. Genetical Research 66: 17.Google Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning A Laboratory Manual. Cold Spring Harbor NY: Cold Spring Harbor Laboratory, pp. 463466.Google Scholar
Tan, YD, Wan, CL, Zhu, YF, Lu, C and Xiang, ZH (2001) An amplified fragment length polymorphism map of the silkworm. Genetics 157: 12771284.Google Scholar
Wan, CL, Tan, YD, Zhu, YF and Lu, C (1999) The application of AFLP markers in the investigation of Bombyx mori genetic diversity. Biotechnology 9 (5): 49 (in Chinese with English abstract).Google Scholar
Wan, CL, Tan, YD, Zhu, YF et al. , (2001) Construction of molecular map of the silkworm (Bombyx mori L.) using AFLP markers. Scientia Agriculltura Sinica 34 (3): 338341 (in Chinese with English abstract).Google Scholar
Xu, YB and Shen, ZT (1992) Detection and genetic analysis of the gene dispersed cross some theoretical considerations. Acta Agriculturae Universitatis ZheJangesis 13 (1): 109117 (in Chinese with English abstract).Google Scholar
Yuji Yasukochi, (1998) A dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics 150: 15131525.Google Scholar
Zabeau, M and Vos, P (1992). Selective restriction fragment amplification: A general method for DNA fingerprinting. European Patent Application, 92402629.7 (Publ. N. 0 534 858 A1).Google Scholar
Zhu, YF, Tan, YD, Wan, CL et al. , (2001) Construction of AFLP linkage map of the silkworm (Bombyx mori). Acta Entomologia Sinica 44 (4): 483493 (in Chinese with English abstract).Google Scholar