Hostname: page-component-76fb5796d-vfjqv Total loading time: 0 Render date: 2024-04-26T19:28:40.628Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular cloning and polymorphism of the porcine H2AFZ gene

Published online by Cambridge University Press:  01 June 2009

Y. H. Zhang ,
S. Q. Mei ,
X. W. Peng ,
B. Zuo ,
M. G. Lei ,
Y. Z. Xiong  and
F. E. Li
Y. H. Zhang
Affiliation:
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
S. Q. Mei
Affiliation:
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Academy of Agriculture Science, Wuhan 430209, P.R. China
X. W. Peng
Affiliation:
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Academy of Agriculture Science, Wuhan 430209, P.R. China
B. Zuo
Affiliation:
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
M. G. Lei
Affiliation:
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
Y. Z. Xiong
Affiliation:
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
F. E. Li*
Affiliation:
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R. China
*
E-mail: lifener@mail.hzau.edu.cn
Get access

Abstract

H2A histone family, member Z (H2A.Z) is required for early mammalian development. In the present study, the 932 bp of full-length cDNA encoding a 128 amino-acid protein and the sequences of intron 2 to 4 of the porcine H2A histone family, member Z (pH2AFZ) gene were obtained. By comparative sequencing of pH2AFZ gene in Large White and Meishan pigs, a 4 bp deletion/insertion in intron 2 was detected and a PCR-Bsu15I-RFLP was established to detect this variation. In DIV (4th Dam line of Chinese lean-type new lines) pigs, the first-parity females with AA genotype had fewer piglets born alive (−2.64 and −1.83 piglets per litter) than those with AB (P < 0.01) and BB (P < 0.05) genotype. The additive allelic and dominance effect were estimated to be 0.92 (P < 0.05) and −0.87 piglets per litter (P < 0.01) for number of piglets born alive, respectively. This result suggests that the pH2AFZ gene might be a good candidate gene of litter-size trait and provides some marker information for marker-assisted selection.

Keywords

pigH2AFZ genelitter sizePCR-Bsu15I-RFLPovary
Type
Full Paper
Information
animal , Volume 3 , Issue 6 , June 2009 , pp. 779 - 782
Copyright
Copyright © The Animal Consortium 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ball, DJ, Slaughter, CA, Hensley, P, Garrard, WT 1983. Amino acid sequence of the N-terminal domain of calf thymus histone H2A.Z. FEBS Letters 154, 166–170.CrossRefGoogle ScholarPubMed
Bruce, K, Myers, FA, Mantouvalou, E, Lefevre, P, Greaves, I, Bonifer, C, Tremethick, DJ, Thorne, AW, Crane-Robinson, C 2005. The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken. Nucleic Acids Research 33, 5633–5639.CrossRefGoogle Scholar
Cassady, JP, Johnson, RK, Pomp, D, Rohrer, GA, Van Vleck, LD, Spiegel, EK, Gilson, KM 2001. Identification of quantitative trait loci affecting reproduction in pigs. Journal of Animal Science 79, 623–633.CrossRefGoogle ScholarPubMed
Faast, R, Thonglairoam, V, Schulz, TC, Beall, J, Wells, JR, Taylor, H, Matthaei, K, Rathjen, PD, Tremethick, DJ, Lyons, I 2001. Histone variant H2A.Z is required for early mammalian development. Current Biology 11, 1183–1187.CrossRefGoogle ScholarPubMed
Guillemette, B, Bataille, AR, Gévry, N, Adam, M, Blanchette, M, Robert, F, Gaudreau, L 2005. Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning. PLoS Biology 3, e384.CrossRefGoogle Scholar
Hatch, CL, Bonner, WM 1988. Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species. Nucleic Acids Research 16, 1113–1124.CrossRefGoogle ScholarPubMed
Hatch, CL, Bonner, WM 1990. The human histone H2A.Z gene. Sequence and regulation. Journal of Biological Chemistry 265, 15211–15218.CrossRefGoogle ScholarPubMed
Hatch, CL, Bonner, WM 1995. Characterization of the proximal promoter of the human histone H2A.Z gene. DNA and Cell Biology 14, 257–266.CrossRefGoogle ScholarPubMed
Jiang, Z, He, H, Hamasima, N, Suzuki, H, Gibbins, AMV 2002. Comparative mapping of Homo sapiens chromosome 4 (HSA4) and Sus scrofa chromosome 8 (SSC8) using orthologous genes representing different cytogenetic bands as landmarks. Genome 45, 147–156.CrossRefGoogle ScholarPubMed
Kamakaka, RT, Biggins, S 2005. Histone variants: deviants? Genes & Development 19, 295–310.CrossRefGoogle ScholarPubMed
King, AH, Jiang, Z, Gibson, JP, Haley, CS, Archibald, AL 2003. Mapping quantitative trait loci affecting female reproductive traits on porcine chromosome 8. Biology of Reproduction 68, 2172–2179.CrossRefGoogle ScholarPubMed
Liu, BH 1998. Statistical genomics: linkage, mapping, and QTL analysis. CRC Press LLC, Boca Raton, Florida, USA.Google Scholar
Raisner, RM, Hartley, PD, Meneghini, MD, Bao, MZ, Liu, CL, Schreiber, SL, Rando, OJ, Madhani, HD 2005. Histone variant H2A.Z marks the 5′ ends of both active and inactive genes in euchromatin. Cell 123, 233–248.CrossRefGoogle ScholarPubMed
Rathje, TA, Rohrer, GA, Johnson, RK 1997. Evidence for quantitative trait loci affecting ovulation rate in pigs. Journal of Animal Science 75, 1486–1494.CrossRefGoogle ScholarPubMed
Rohrer, GA, Ford, JJ, Wise, TH, Vallet, JL, Christenson, RK 1999. Identification of quantitative trait loci affecting female reproductive traits in a multigeneration Meishan–White composite swine population. Journal of Animal Science 77, 1385–1391.CrossRefGoogle Scholar
Van Laere, AS, Nguyen, M, Braunschweig, M, Nezer, C, Collette, C, Moreau, L, Archibald, AL, Haley, CS, Buys, N, Tally, M, Andersson, G, Georges, M, Andersson, L 2003. A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature 425, 832–836.CrossRefGoogle Scholar