Hostname: page-component-848d4c4894-ndmmz Total loading time: 0 Render date: 2024-05-11T14:36:39.862Z Has data issue: false hasContentIssue false
  • English
  • Français

Polypyrrole - A Potential Candidate for Stimulated Nerve Regeneration

Published online by Cambridge University Press:  15 February 2011

V. R. Shastri ,
C. E. Schmidt ,
T.-H. Kim ,
J. P. Vacanti  and
R. Langer
V. R. Shastri
Affiliation:
Department of Chemical Engineering, MIT, Cambridge, MA 02139
C. E. Schmidt
Affiliation:
Department of Chemical Engineering, MIT, Cambridge, MA 02139
T.-H. Kim
Affiliation:
Department of Surgery, Children's Hospital, Harvard University, Boston, MA 02115
J. P. Vacanti
Affiliation:
Department of Surgery, Children's Hospital, Harvard University, Boston, MA 02115
R. Langer
Affiliation:
Department of Chemical Engineering, MIT, Cambridge, MA 02139 Department of Surgery, Children's Hospital, Harvard University, Boston, MA 02115
Get access

Abstract

The proposed research is aimed at gaining a fundamental understanding of in vitro nerve cellbiomaterials interactions, with the immediate goal of engineering materials to specifically enhance expression of the neuronal phenotype. Long term goals include (1) optimizing the innervation (connection) of the host neurons with implanted bioartificial tissue constructs and, (2) facilitating the regeneration of damaged peripheral and central nerves. In this study, the interactions of neuronal like PC-I12 cells with the electrically conducting polymer, polypyrrole, (PP) have been investigated. It has been shown by quantitative image analysis that neurite extensions of PC-12 cells are more pronounced on PP surfaces as compared to tissue culture polystyrene. Application of an electrical stimulus to cells cultured on PP film significantly increased (based on morphological evaluation) the expression of neurites in these cells compared to controls. Tissue compatibility and transected sciatic nerve regeneration studies in rat models show that PP films invoke little negative response and support nerve regeneration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 113
Copyright
Copyright © Materials Research Society 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Kiyochi, H., Ono, A., Yamamoto, N., Ohnishi, K., Shimahara, Y., Kobayashi, N., Transplantation 59, 328 (1995).Google Scholar
2. Aebischer, P., Valentini, R. F., Dario, P., Domenici, C., Galletti, P. M., Brain. Res. 436, 165 (1987).Google Scholar
3. Valentini, R. F., Vargo, T. C., Gardella, J. A. Jr., Aebischer, P., Biomaterials 13, 183 (1992).Google Scholar
4. Valentini, R. F., Sabatini, A. M., Dario, P., Aebischer, P., Brain. Res. 480, 300 (1989).Google Scholar
5. Kerns, J. M., Fakhouri, A. J., Weinrib, H. P., Freeman, J. A., Neuroscience 40, 93 (1991).Google Scholar
6. Politis, M. J., Zanakis, M. F., Albala, B. J., J. Trauma 28, 1548 (1988).Google Scholar
7. Sisken, B. F., Kanje, M., Lundborg, G., Herbst, E., Kurtz, W., Brain. Res. 485, 309 (1989).Google Scholar
8. Wong, J. Y., Langer, R., Ingber, D. E., Proc. Natl. Acad. Sci. USA. 91, 3201 (1994).Google Scholar
9. Shastri, V. R., Ph. D. Thesis, Rensselaer Polytechnic Institute, 1995.Google Scholar
10. Diaz, A. F., Castillo, J. C., Logan, J. A., Lee, W. Y., J. Electroanal. Chem. 129, 115 (1981).Google Scholar
11. The PLGA films were cast from a solution (100 mg/ml) in dichloromethane into 30 ml beakers (1 ml per beaker, 7.55 cm 2). After 24 hours the films were removed from the bottom of the beakers by floating them off in water. The free floating films were then dried under vacuum for 24 hours before further use.Google Scholar
12. Greene, L., Tischler, A., Proc. Natl. Acad. Sci. USA. 73, 2424 (1976).Google Scholar
13. Jaffe, L. F., Poo, M.-M., J.Exp. Zool. 209, 115(1979).Google Scholar
14. Patel, N. B., Poo, M.-M., J. Neurosc. 4, 2939 (1984).Google Scholar
15. Shibib, K., Brock, M., Buljat, G., Gosztonyi, G., Schoknecht, G., Surg. Neurol. 29, 372 (1988).Google Scholar
16. Luther, P. W., Peng, H. B., Lin, J. J.-C., Nature 303, 61 (1983).Google Scholar