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Effect of 2,4-D on the Hydrolysis of Diclofop-Methyl in Wild Oat (Avena fatua)

Published online by Cambridge University Press:  12 June 2017

B. D. Hill ,
B. G. Todd  and
E. H. Stobbe
B. D. Hill
Affiliation:
Plant Sci. Dep., Univ. of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
B. G. Todd
Affiliation:
Plant Sci. Dep., Univ. of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
E. H. Stobbe
Affiliation:
Plant Sci. Dep., Univ. of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
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Abstract

The basis for 2,4-D [(2,4-dichlorophenoxy)acetic acid] antagonism of diclofop-methyl {methyl 2-[4-(2,4-dichlorophenoxy) phenoxy] propanoate} toxicity to wild oat (Avena fatua L.) was investigated by studying changes in the metabolism of diclofop-methyl in vitro. An esterase from wild oat, which hydrolyzes diclofop-methyl to the acid diclofop, was extracted, partially purified, and the reaction characterized. The rate of hydrolysis of 14C-diclofop-methyl was 0.14 ηmoles/2 h at standard assay conditions of 0.25 mg lyophilized enzyme preparation (19.6% protein) in 0.1 ml phosphate buffer (0.1 M, pH 7.0), substrate 5 μM. The addition of 2,4-D to this reaction did not inhibit 14C-diclofop formation. Higher levels of 2,4-D stimulated enzymic hydrolysis. 14C-diclofop-methyl was rapidly metabolized to 14C-diclofop and polar 14C-conjugates when vacuum-infiltrated into wild oat leaf segments. The addition of 2,4-D caused small increases in the rates of both 14C-diclofop-methyl de-esterification and 14C-diclofop conjugation. It is concluded that 2,4-D does not inhibit the in vitro de-esterification of diclofop-methyl.

Keywords

Metabolismantagonismin vitroesterase
Type
Research Article
Information
Weed Science , Volume 28 , Issue 6 , November 1980 , pp. 725 - 729
Copyright
Copyright © Weed Science Society of America 

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References

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