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Biotinylated Polythiophene Copolymer – a Novel Electroactive Biomaterial Utilizing the Biotin-Streptavidin Interaction

Published online by Cambridge University Press:  15 February 2011

Jeong-Ok Lim ,
Manjunath Kamath ,
Kenneth A. Marx ,
Sukant K. Tripathy ,
David L. Kaplan  and
Lynne A. Samuelson
Jeong-Ok Lim
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Manjunath Kamath
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Kenneth A. Marx
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
Sukant K. Tripathy
Affiliation:
Center for Advanced Materials, Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01854
David L. Kaplan
Affiliation:
Biotechnology Division, U.S. Army Natick Research, Development & Engineering Center, Natick, Massachusetts 01760
Lynne A. Samuelson
Affiliation:
Biotechnology Division, U.S. Army Natick Research, Development & Engineering Center, Natick, Massachusetts 01760
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Abstract

A novel hierarchical biomaterial capable of incorporating any biotinylated biomolecule has been created. Our strategy is to biotinylate one-dimensional electroactive polymers and use a bridging streptavidin protein on Langmuir-Blodgett (LB) organized films. The following copolymeric system which enables functionalization of other molecules and formation of good monolayers was employed. Biotinylated poly(3-methanolthiophene-co-3-undecylthiophene) (B-PMUT) demonstrated a significantly better isotherm implying superior molecular packing compared to poly(3-methanolthiophene-co-3-undecylthiophene) (PMUT) on the LB airwater surface. The isotherm showed significant area expansion when streptavidin was injected below the B-PMUT monolayer in 0.1mM NaH2PO4/0.1 M NaCl buffer (pH 6.8) subphase. We then incorporated biotinylated phycoerythrin (B-PE) into this novel biomaterial by binding the unoccupied biotin binding sites on the bound streptavidin (4 sites total). The pressure-area isotherm of the protein injected monolayer showed area expansion. A characteristic fluorescent emission peak at 576nm was detected from the monolayer transferred onto a solid substrate. These observations demonstrated the function of B-PMUT in hierarchical monolayer assembly of molecules incorporating the biotin / streptavidin interaction.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 292: Symposium S – Biomolecular Materials , 1992 , 141
Copyright
Copyright © Materials Research Society 1993

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