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Three-dimensional nanofiber scaffolds with arrayed holes for engineering skin tissue constructs

Published online by Cambridge University Press:  04 July 2017

Lina Fu ,
Jingwei Xie ,
Mark A. Carlson  and
Debra A. Reilly
Lina Fu
Affiliation:
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA
Jingwei Xie*
Affiliation:
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA
Mark A. Carlson
Affiliation:
Department of Surgery-General Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA
Debra A. Reilly
Affiliation:
Department of Surgery-Plastic Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA
*
Address all correspondence to J. Xie at jingwei.xie@unmc.edu
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Abstract

Three-dimensional (3D) scaffolds composed of poly(ε-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor improved the water stability of the scaffolds. Cell spheroids of green fluorescent protein-labeled human dermal fibroblasts were prepared and seeded into the holes. It was found that the fibroblasts adhered well on the surface of nanofibers and migrated into the scaffolds due to the porous structures. The 3D nanofiber scaffolds may hold great potential for engineering tissue constructs for various applications.

Type
Biomaterials for 3D Cell Biology Research Letters
Information
MRS Communications , Volume 7 , Issue 3 , September 2017 , pp. 361 - 366
Copyright
Copyright © Materials Research Society 2017 

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