Hostname: page-component-848d4c4894-wzw2p Total loading time: 0 Render date: 2024-05-08T06:34:53.516Z Has data issue: false hasContentIssue false
  • English
  • Français

Lubricin as a Surface Treatment to Reduce Post-operative Biofouling and Infection

Published online by Cambridge University Press:  22 November 2012

George E. Aninwene II ,
Doug Hall ,
Amy Mei ,
Gregory D. Jay  and
Thomas J. Webster
George E. Aninwene II
Affiliation:
School of Engineering, Brown University, Providence, R I 02912 USA
Doug Hall
Affiliation:
School of Engineering, Brown University, Providence, R I 02912 USA
Amy Mei
Affiliation:
School of Engineering, Brown University, Providence, R I 02912 USA
Gregory D. Jay
Affiliation:
Department of Medicine, Section of Emergency Medicine, Brown University, School of Medicine, Providence, R I 02912 USA
Thomas J. Webster
Affiliation:
School of Engineering, Brown University, Providence, R I 02912 USA Department of Orthopaedics, Brown University, Providence, RI 02912 USA
Get access

Abstract

The goal of this research was to investigate the ability of lubricin to prevent bio-fouling of intraocular lenses after surgery, through surface coating trials with lubricin and analogues of the two major sub-units of the lubricin molecule (mucin and vitronectin). Yearly, there are over 6 million surgeries worldwide that involve intraocular lenses (IOLs) 1. However, preventing post-operative biofouling and bacterial infection of these implants remains a challenge 2. Surface modification of IOLs may provide a solution. This study proposes the use of the anti-adhesive protein lubricin (LUB), a glycoprotein found in the synovial fluid, as a means to make polymer surfaces less prone to bacterial adhesion and proliferation; thus, reducing the opportunity for post-operative infection 3. This study used extended bacteria growth trials on tissue cultures polystyrene coated with either lubricin, vitronectin, or mucin to investigate how lubricin and protein sub-regions of lubricin may reduce bacterial adhesion and proliferation.

Keywords

Adhesionbiomedicalcoating
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1486: Symposium S4A – Nanotechnology-enhanced Biomaterials and Biomedical Devices , 2012 , imrc12-1486-s4a-o002
Copyright
Copyright © Materials Research Society 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Werner, L., Journal of Cataract & Refractive Surgery 33 (4), 713–726 (2007).CrossRefGoogle Scholar
Kodjikian, L., Burillon, C., Chanloy, C., Bostvironnois, V., Pellon, G., Mari, E., Freney, J. and Roger, T., 2002 (unpublished).Google Scholar
Zappone, B., Ruths, M., Greene, G. W., Jay, G. D. and Israelachvili, J. N., Biophys. J. 92 (5), 1693–1708 (2007).CrossRefGoogle Scholar
Puckett, S. D., Taylor, E., Raimondo, T. and Webster, T. J., Biomaterials 31 (4), 706–713 (2010).CrossRefGoogle Scholar
Weber, D. J., Hoffman, K. L., Thoft, R. A. and Baker, A. S., Review of Infectious Diseases 8 (1), 12–20 (1986).CrossRefGoogle Scholar
Gill, S. R., Fouts, D. E., Archer, G. L., Mongodin, E. F., DeBoy, R. T., Ravel, J., Paulsen, I. T., Kolonay, J. F., Brinkac, L., Beanan, M., Dodson, R. J., Daugherty, S. C., Madupu, R., Angiuoli, S. V., Durkin, A. S., Haft, D. H., Vamathevan, J., Khouri, H., Utterback, T., Lee, C., Dimitrov, G., Jiang, L., Qin, H., Weidman, J., Tran, K., Kang, K., Hance, I. R., Nelson, K. E. and Fraser, C. M., J. Bacteriol. 187 (7), 2426–2438 (2005).CrossRefGoogle Scholar
Lowy, F. D., New England Journal of Medicine 339 (8), 520–532 (1998).CrossRefGoogle Scholar
Costerton, J. W., Stewart, P. S. and Greenberg, E. P., Science 284 (5418), 1318–1322 (1999).CrossRefGoogle Scholar
Kluytmans, J., van Belkum, A. and Verbrugh, H., Clin. Microbiol. Rev. 10 (3), 505–520 (1997).CrossRefGoogle Scholar
Chang, D. P., Abu-Lail, N. I., Guilak, F., Jay, G. D. and Zauscher, S., Langmuir 24 (4), 1183–1193 (2008).CrossRefGoogle Scholar
Jay, G. D., Harris, D. A. and Cha, C.-J., Glycoconjugate Journal 18 (10), 807–815 (2001).CrossRefGoogle Scholar
Jay, G. D., Torres, J. R., Warman, M. L., Laderer, M. C. and Breuer, K. S., Proc. Natl. Acad. Sci. U. S. A. 104 (15), 6194–6199 (2007).CrossRefGoogle Scholar
Shi, L., Ardehali, R., Caldwell, K. D. and Valint, P., Colloids and Surfaces B: Biointerfaces 17 (4), 229–239 (2000).CrossRefGoogle Scholar
Rhee, D. K., Marcelino, J., Baker, M., Gong, Y., Smits, P., Lefebvre, V. r., Jay, G. D., Stewart, M., Wang, H., Warman, M. L. and Carpten, J. D., The Journal of Clinical Investigation 115 (3), 622–631 (2005).CrossRefGoogle Scholar