Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-26T09:59:06.643Z Has data issue: false hasContentIssue false
  • English
  • Français

Histology, histochemistry and enzyme biochemistry of the digestive glands in the tropical surf barnacle Tetraclita squamosa

Published online by Cambridge University Press:  11 May 2009

D. J. Johnston ,
C. G. Alexander  and
D. Yellowlees
D. J. Johnston
Affiliation:
Department of Marine Biology;
C. G. Alexander
Affiliation:
Department of Marine Biology;
D. Yellowlees
Affiliation:
Department of Chemistry and Biochemistry, James Cook University of North Queensland, Townsville, Queensland 4811, Australia
Get access Rights & Permissions [Opens in a new window]

Extract

The tropical surf barnacle Tetradita squamosa (Bruguière) (Crustacea: Cirripedia) has a pair of lobulate digestive glands each connected to the dorsal surface of the foregut by a single duct. These glands have a central lumen surrounded by columnar epithelial cells and are the primary site of enzyme synthesis and secretion within the alimentary tract. A number of carbohydrases and proteases were detected, all of which exhibited optimal activity at an acidic pH. Gland cells discharge secretory products into the foregut ventriculus via apocrine secretion and this process is continuous; it is not correlated with tidal and subsequent feeding cycles. Possible reasons for the absence of feeding and secretory co-ordination are discussed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 73 , Issue 1 , February 1993 , pp. 1 - 14
Copyright
Copyright © Marine Biological Association of the United Kingdom 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Achituv, Y., 1981. The biochemical composition of the nauplius stages of Tetraclita squamosa rufotincta Pilsbry. Journal of Experimental Marine Biology and Ecology, 51, 241–246.Google Scholar
Al-Mohanna, S. Y., Nott, J. A. & Lane, D. J. W., 1985 a. M-‘midget’ cells in the hepatopancreas of the shrimp, Penaeus semisulcatus De Haan, 1844 (Decapoda, Natantia). Crustaceana, 48,260–268.CrossRefGoogle Scholar
Al-Mohanna, S. Y., Nott, J. A. & Lane, D. J. W., 1985 b. Mitotic E- and secretory F-cells in the hepatopancreas of the shrimp Penaeus semisulcatus (Crustacea: Decapoda). Journal of the Marine Biological Association of the United Kingdom, 65, 901–910.CrossRefGoogle Scholar
Anderson, D. T. & Buckle, J., 1983. Cirral activity and feeding in the coronuloid barnacles Tesseropora rosea (Krauss) and Tetraclitella purpurascens (Wood) (Tetraclitidae). Bulletin of Marine Science, 33, 645–655.Google Scholar
Anderson, D. T. & Southward, A. J., 1987. Cirral activity of barnacles. In Barnacle biology. Crustacean issues, vol. 5 (ed. Southward, A. J.), pp. 135–174. Rotterdam: A.A. Balkema.Google Scholar
Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248–254.CrossRefGoogle ScholarPubMed
Bull, A. T. & Chesters, C. G. C., 1966. The biochemistry of laminarin and the nature of laminarinase. Advances in Enzymology, 28, 325–364.Google ScholarPubMed
Caceci, T., Neck, K. F., Lewis, D. H. & Sis, R. F., 1988. Ultrastructure of the hepatopancreas of the Pacific white shrimp, Penaeus vannemei (Crustacea: Decapoda). Journal of the Marine Biological Association of the United Kingdom, 68, 323–337.CrossRefGoogle Scholar
Carr, K. E. & Toner, P. G. 1982. Cell structure: an introduction to biomedical electron microscopy. New York: Churchill Livingstone.Google Scholar
Clayton, M. N. & King, R. J., 1981. Marine botany: an Australian perspective, pp. 138–179. Melbourne: Longman Cheshire.Google Scholar
Cook, H. C., 1974. Manual of histological demonstration techniques. Brisbane: Butterworths.Google Scholar
Crisp, D. J., 1976. Settlement responses in marine organisms. In Adaptation to the environment: essays on the physiology of marine animals (ed. Newell, R. C.), pp. 83–124. London: Butterworths.CrossRefGoogle Scholar
Dall, W. & Moriarty, D. J. W., 1983. Functional aspects of nutrition and digestion. In The biology of Crustacea, vol. 5. Internal anatomy and physiological regulation (ed. Mantel, L. H.), pp. 215–251. New York: Academic Press.Google Scholar
Devillez, E. J., 1975. Observations on the proteolytic enzymes in the digestive fluid of the barnacle Balanus nubilus. Comparative Biochemistry and Physiology, 51A, 471–474.Google Scholar
Devillez, E. & Buschlen, K., 1967. Survey of a tryptic digestive enzyme in various species of Crustacea. Comparative Biochemistry and Physiology, 21, 541–546.Google Scholar
Easton, A. K., 1970. The tides of the continent of Australia. Horace Lamb Centre for Oceanographical Research, 37, 1–326.Google Scholar
Elendt, B.-P. & Storch, V., 1990. Starvation-induced alterations of the ultrastructure of the midgut of Daphnia magna Straus, 1820 (Cladocera). journal of Crustacean Biology, 10, 79–86.Google Scholar
Elyakova, L. A., 1972. Distribution of cellulases and chitinases in marine invertebrates. Comparative Biochemistry and Physiology, 43B, 67–70.Google Scholar
Foster, B. A., 1987. Barnacle ecology and adaptation. In Barnacle biology. Crustacean issues, vol. 5 (ed. Southward, A. J.), pp. 113–133. Rotterdam: A.A. Balkema.Google Scholar
Galgani, F. G., Benyamin, Y. & Ceccaldi, H. J. 1984. Identification of digestive proteinases of Penaeus kerathurus (Forskål): a comparison with Penaeus japonicus Bate. Comparative Biochemistry and Physiology, 78B, 355–361.Google Scholar
Gates, B. J. & Travis, J., 1973. Purification and characterization of carboxypeptidases A and B from the white shrimp (Penaeus setiferus). Biochemistry, 12, 1867–1874.CrossRefGoogle Scholar
Gibson, R. & Barker, P. L., 1979. The decapod hepatopancreas. Oceanography and Marine Biology. Annual Review. London, 17, 285–346.Google Scholar
Harnden, D. G., 1968. Digestive carbohydrases of Balanus nubilus (Darwin, 1854). Comparative Biochemistry and Physiology, 25, 303–309.Google Scholar
Hui, E. & Moyse, J., 1987. Settlement patterns and competition for space. In Barnacle Biology. Crustacean issues, vol. 5 (ed. Southward, A. J.), pp. 363–376. Rotterdam: A.A. Balkema.Google Scholar
Hummel, B. C. W., 1959. A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin. Canadian Journal of Biochemistry and Physiology, 37, 1393–1399.CrossRefGoogle ScholarPubMed
Hunt, M. J. & Alexander, C. G., 1991. Feeding mechanisms in the barnacle Tetraclita squamosa (Bruguière). Journal of Experimental Marine Biology and Ecology, 154, 1–28.CrossRefGoogle Scholar
Kristensen, J. H., 1972. Carbohydrases of some marine invertebrates with notes on their food and on the natural occurrence of the carbohydrates studied. Marine Biology, 14, 130–142.Google Scholar
Lillie, R. D. & Fullmer, H. M., 1976. Histopathologic technic and practical histochemistry, 4th ed.United States of America: McGraw-Hill Book Company.Google Scholar
Merdsoy, B. & Farley, J. 1973. Phasic activity in the digestive gland cells of the marine prosobranch gastropod, Littorina littorea (L.). Proceedings of the Malacological Society of London, 40, 473–482.Google Scholar
Monk, D. C., 1976. The distribution of cellulase in freshwater invertebrates of different feeding habits. Freshwater Biology, 6, 471–475.Google Scholar
Nott, J. A., 1969. Settlement of barnacle larvae: surface structure of the antennular attachment disc by scanning electron microscopy. Marine Biology, 2, 248–251.CrossRefGoogle Scholar
Otway, N. M. & Underwood, A. J., 1987. Experiments on orientation of the intertidal barnacle Tesseropora rosea (Krauss). Journal of Experimental Marine Biology and Ecology, 105, 85–106.CrossRefGoogle Scholar
Percival, E., 1970. Algal polysaccharides. In The carbohydrates, 2B (ed. Pigman, W. and Horton, D.), pp. 537–565. New York: Academic Press.Google Scholar
Rainbow, P. S. & Walker, G. 1977. The functional morphology of the alimentary tract of barnacles (Cirripedia: Thoracica). journal of Experimental Marine Biology and Ecology, 28, 183–206.Google Scholar
Reynolds, E. S., 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, journal of Cell Biology, 17, 208–212.Google Scholar
Rinderknecht, H., Wilding, P. & Haverback, B. J., 1967. A new method for the determination of aamylase. Experientia, 23, 805.CrossRefGoogle Scholar
Southward, A. J. & Crisp, D. J., 1965. Activity rhythms of barnacles in relation to respiration and feeding, journal of the Marine Biological Association of the United Kingdom, 45, 161–185.CrossRefGoogle Scholar
Spurr, A. R., 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research, 26, 31–43.CrossRefGoogle ScholarPubMed
Tornava, S. R., 1948. The alimentary canal of Balanus improvisus Darwin. Acta Zoologica Fennica, 52, 1–52.Google Scholar
Trevelyan, W. E., Procter, D. P. & Harrison, J. S., 1950. Detection of sugars on paper chromatograms. Nature, London, 166, 444–445.Google Scholar
Vogt, G., Stöcker, W., Storch, V. & Zwilling, R., 1989. Biosynthesis of Astacus protease, a digestive enzyme from crayfish. Histochemistry, 91, 373–381.Google Scholar
Weel, P. B. Van, 1970. Digestion in crustacea. In Chemical zoolog, vol. 5. Arthropoda, part A (ed. Florkin, M. B. and Scheer, B. T.), pp. 97–115. New York: Academic Press.Google Scholar
Winsor, L., 1984. Manual of basic zoological microtechniques for light microscopy (revised edition). Australia: James Cook University of North Queensland.Google Scholar
Zwilling, R. & Neurath, H., 1981. Invertebrate proteases. Methods in Enzymology, 80, 633–664.CrossRefGoogle Scholar