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Effects of branched-chain volatile fatty acids on lactation performance and mRNA expression of genes related to fatty acid synthesis in mammary gland of dairy cows

Published online by Cambridge University Press:  12 February 2018

Q. Liu ,
C. Wang ,
G. Guo ,
W. J. Huo ,
S. L. Zhang ,
C. X. Pei ,
Y. L. Zhang  and
H. Wang
Q. Liu*
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
C. Wang
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
G. Guo
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
W. J. Huo
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
S. L. Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
C. X. Pei
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
Y. L. Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801 Shanxi Province, P.R. China
H. Wang
Affiliation:
Animal Husbandry and Veterinary Bureau of Yuci County, Yuci, 030600 Shanxi Province, P.R. China
*
†E-mail: liuqiangabc@163.com
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Abstract

Branched-chain volatile fatty acids (BCVFA) supplements could promote lactation performance and milk quality by improving ruminal fermentation and milk fatty acid synthesis. This study was conducted to evaluate the effects of BCVFA supplementation on milk performance, ruminal fermentation, nutrient digestibility and mRNA expression of genes related to fatty acid synthesis in mammary gland of dairy cows. A total of 36 multiparous Chinese Holstein cows averaging 606±4.7 kg of BW, 65±5.2 day in milk (DIM) with daily milk production of 30.6±0.72 kg were assigned to one of four groups blocked by lactation number, milk yield and DIM. The treatments were control, low-BCVFA (LBCVFA), medium-BCVFA (MBCVFA) and high-BCVFA (HBCVFA) with 0, 30, 60 and 90 g BCVFA per cow per day, respectively. Experimental periods were 105 days with 15 days of adaptation and 90 days of data collection. Dry matter (DM) intake tended to increase, but BW changes were similar among treatments. Yields of actual milk, 4% fat corrected milk, milk fat and true protein linearly increased, but feed conversion ratio (FCR) linearly decreased with increasing BCVFA supplementation. Milk fat content linearly increased, but true protein content tended to increase. Contents of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0 and C15:0 fatty acids in milk fat linearly increased, whereas other fatty acids were not affected with increasing BCVFA supplementation. Ruminal pH, ammonia N concentration and propionate molar proportion linearly decreased, but total VFA production and molar proportions of acetate and butyrate linearly increased with increasing BCVFA supplementation. Consequently, acetate to propionate ratios linearly increased. Digestibilities of DM, organic matter, CP, NDF and ADF also linearly increased. In addition, mRNA expressions of peroxisome proliferator-activated receptor γ, sterol regulatory element-binding factor 1 and fatty acid-binding protein 3 linearly increased, mRNA expressions of acetyl-coenzyme A carboxylase-α, fatty acid synthase and stearoyl-CoA desaturase quadratically increased. However, lipoprotein lipase mRNA expression was not affected by treatments. The results indicated that lactation performance and milk fat synthesis increased with BCVFA supplementation by improving ruminal fermentation, nutrient digestibility and mRNA expressions of genes related to milk fat synthesis.

Keywords

branched-chain volatile fatty acidsruminal fermentationnutrient digestibilitymilk fat synthesisgene expression
Type
Research Article
Information
animal , Volume 12 , Issue 10 , October 2018 , pp. 2071 - 2079
Copyright
© The Animal Consortium 2018 

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References

Allen, MS 2000. Effects of diet on short-term regulation of feed intake by lactating dairy cattle. Journal of Dairy Science 83, 1598–1624.Google Scholar
Association of Official Analytical Chemists (AOAC) 1997. Official methods of analysis, 16th edition. AOAC, Washington, DC, USA.Google Scholar
Bernard, L, Leroux, C and Chilliard, Y 2008. Expression and nutritional regulation of lipogenic genes in the ruminant lactating mammary gland. Advances in Experimental Medicine and Biology 606, 67–108.Google Scholar
Bionaz, M and Loor, J 2008. Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9, 366.Google Scholar
Chouinard, PY, Corneau, L, Barbano, DM, Metzger, LE and Bauman, DE 1999. Conjugated linoleic acids alter milk fatty acid composition and inhibit milk fat secretion in dairy cows. Journal of Nutrition 129, 1579–1584.Google Scholar
Cummins, KA and Papas, AH 1985. Effect of isocarbon 4 and isocarbon 5 volatile fatty acids on microbial protein synthesis and dry matter digestibility in vitro. Journal of Dairy Science 68, 2588–2595.Google Scholar
Denman, SE and McSweeney, CS 2006. Developmentofa real-time PCR assay formonitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology 58, 572–582.Google Scholar
Farr, VC, Stelwagen, K, Cate, LR, Molenaar, AJ, McFadden, TB and Davis, SR 1996. An improved method for the routine biopsy of bovine mammary tissue. Journal of Dairy Science 79, 543–549.Google Scholar
Ferret, A, Plaixats, J, Caja, G, Gasa, J and Prió, P 1999. Using markers to estimate apparent dry matter digestibility, faecal output and dry matter intake in dairy ewes fed Italian ryegrass hay or alfalfa hay. Small Ruminant Research 33, 145–152.Google Scholar
Formigoni, A, Martelli, G and Parisini, P 1995. Effect of administration of isoacids to dairy cows on milk destined for cheesemaking. Atti della Società Italiana di Buiatria 27, 87–97 (in Italian).Google Scholar
Firkins, JL, Yu, Z and Morrison, M 2007. Ruminal nitrogen metabolism: perspectives for integration of microbiology and nutrition for dairy. Journal of Dairy Science 90, E1–E16.Google Scholar
Gunter, SA, Krysl, LJ, Judkins, MB, Broesder, JT and Barton, RK 1990. Influence of branched-chain fatty acid supplementation on voluntary intake, site and extent of digestion, ruminal fermentation, digesta kinetics and microbial protein synthesis in beef heifers consuming grass hay. Journal of Animal Science 68, 2885.Google Scholar
Gylswyk, NOV 1970. The effect of supplementing a low-protein hay on the cellulolytic bacteria in the rumen of sheep and on the digestibility of cellulose and hemicellulose. Journal of Agricultural Science 74, 169–180.Google Scholar
Hara, A and Radin, NS 1978. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry 90, 420–426.Google Scholar
Jacobs, AA, Dijkstra, J, Liesman, JS, Vandehaar, MJ, Lock, AL, van Vuuren, AM, Hendriks, WH and van Baal, J 2013. Effects of short- and long-chain fatty acids on the expression of stearoyl-CoA desaturase and other lipogenic genes in bovine mammary epithelial cells. Animal 7, 1508–1516.Google Scholar
Kamble, DP and Thakur, SS 2004. Effect of branched-chain volatile fatty acid supplementation on rumen fermentation and nutrient utilization in cattle fed urea impregnated wheat straw. Indian Journal of Animal Science 74, 80–83.Google Scholar
Kolver, ES and DeVeth, MJ 2002. Prediction of ruminal pH from pasture-based diets. Journal of Dairy Science 85, 1255–1266.Google Scholar
Kuzinski, J, Zitnan, R, Viergutz, T, Legath, J and Schweige, M 2011. Altered Na+/K+-ATPase expression plays a role in rumen epithelium adaptation in sheep fed hay ad libitum or a mixed hay/concentrate diet. Veterinarni Medicina 56, 35–47.Google Scholar
Lehner, R and Kuksis, A 1996. Biosynthesis of triacylglycerols. Lipid Research 35, 169–201.Google Scholar
Liu, Q, Wang, C, Huang, YX, Dong, K, Yang, WZ and Wang, H 2008. Effects of isobutyrate on rumen fermentation, urinary excretion of purine derivatives and digestibility in steers. Archives of Animal Nutrition 62, 377–388.Google Scholar
Liu, Q, Wang, C, Pei, CX, Li, HY, Wang, YX, Zhang, SL, Zhang, YL, He, JP, Wang, H, Yang, WZ, Bai, YS, Shi, ZG and Liu, XN 2014. Effects of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet. Livestock Science 161, 60–68.Google Scholar
Liu, Q, Wang, C, Zhang, YL, Pei, CX, Zhang, SL, Li, HQ, Guo, G, Huo, WJ, Yang, WZ and Wang, H 2016. Effects of 2-methybutyrate supplementation on growth performance and ruminal development in pre- and post-weaning dairy calves. Animal Feed Science and Technology 216, 129–137.Google Scholar
Liu, Q, Wang, C, Yang, WZ, Zhang, B, Yang, XM, He, DC, Zhang, P, Dong, KH and Huang, YX 2009. Effects of isobutyrate on rumen fermentation, lactation performance and plasma characteristics in dairy cows. Animal Feed Science and Technology 154, 58–67.Google Scholar
Ma, L and Corl, BA 2012. Transcriptional regulation of lipid synthesis in bovine mammary epithelial cells by sterol regulatory element binding protein-1. Journal of Dairy Science 95, 3743–3755.Google Scholar
Mansson, HL 2008. Fatty acids in bovine milk fat. Food Nutrition Research 52, 1–3.Google Scholar
Maxin, G, Glasser, F, Hurtaud, C, Peyraud, J and Rulquin, H 2011. Combined effects of trans-10,cis-12 conjugated linoleic acid, propionate, and acetate on milk fat yield and composition in dairy cows. Journal of Dairy Science 94, 2051–2059.Google Scholar
National Research Council 2001. Nutrient requirements of young calf. In Nutrient requirements of dairy cattle, chapter 10, 7th revised edition (ed. Subcommittee on Dairy Cattle Nutrition, Committee on Animal Nutrition and Board on Agriculture and Natural Resources), pp. 214–233. National Academy of Sciences, Washington, DC, USA.Google Scholar
Ramachandran, M and Das, TK 2000. Effect of branched-chain volatile fatty acid supplementation on growth and dry matter intake in sheep. Indian Journal of Animal Nutrition 17, 320–323.Google Scholar
SAS 2002. User’s guide: statistics, version 9 edition. Statistical Analysis Systems Institute, Cary, NC, USA.Google Scholar
Sheng, R, Yan, SM, Qi, LZ, Zhao, YL, Jin, L and Guo, XY 2015. Effect of the ratios of acetate and β-hydroxybutyrate on the expression of milk fat- and protein-related genes in bovine mammary epithelial cells. Czech Journal of Animal Science 60, 531–541.Google Scholar
Van Soest, PJ, Robertson, JB and Lewis, BA 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Wang, C, Liu, Q, Pei, CX, Li, HY, Wang, YX, Wang, H, Bai, YS, Shi, ZG, Liu, XN and Li, P 2012. Effects of 2-methylbutyrate on rumen fermentation, ruminal enzyme activities, urinary excretion of purine derivatives and feed digestibility in steers. Livestock Science 145, 160–166.Google Scholar
Wang, C, Liu, Q, Zhang, YL, Pei, CX, Zhang, SL, Wang, YX, Yang, WZ, Bai, YS, Shi, ZG and Liu, XN 2015. Effects of isobutyrate supplementation on ruminal microflora, rumen enzyme activities and methane emissions in Simmental steers. Journal of Animal Physiology and Animal Nutrition 99, 123–131.Google Scholar
Williams, CH, David, DJ and Iismaa, O 1962. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science 59, 381–385.Google Scholar
Zhang, YL, Liu, Q, Wang, C, Pei, CX, Li, HY, Wang, YX, Yang, WZ, Bai, YS, Shi, ZG and Liu, XN 2015. Effects of supplementation of simmental steers with 2-methylbutyrate on rumen microflora, enzyme activities and methane production. Animal Feed Science and Technology 199, 84–92.Google Scholar