Macrophages are a heterogeneous population of cells that play a role in the innate immune response to infection. Recent studies have shown that macrophages are key cells in the development of obesity, wherein there is progressive infiltration of macrophages into the adipose tissue. These adipose tissue macrophages are referred to as classically-activated, or M1, macrophages⁽Reference Lumeng, Bodzin and Saltiel¹⁾. They release pro-inflammatory cytokines such as IL-1, IL-6 and TNFα, creating an inflammatory response that can contribute to insulin resistance and type 2 diabetes mellitus (T2DM)⁽Reference Weisburg, McCann, Desai, Rosenbaum, Leibel and Ferrante²⁾. In lean individuals macrophages are in an M2-polarization or alternatively-activated state and are thought to protect against inflammation. The IL-1 receptor type 1 (IL-1R1) is responsible for transmitting the pro-inflammatory effects of IL-1. The aim of the present study was to determine the number of adipose tissue macrophages and their activation state using flow cytometry in control mice and in IL-1R1^−/− mice at certain time points over a period of 12 weeks.

C57BL/6 control and IL-1R1^−/− mice were fed a high-fat diet (45% energy from fat) for 12 weeks. At week 6 epididymal adipose tissue samples were taken from control and IL-1R1^−/− mice. Adipocytes and stromal vascular cells (SVC) were isolated from the adipose tissue by collagenase treatment. SVC were labelled with antibodies for macrophage markers F4/80, CD11B and CD11C and analysed by flow cytometry to determine the number of macrophages and activation status of the macrophages present. Triple-positive cells (F4/80⁺CD11B⁺CD11C⁺) are associated with the M1-polarization state, while double-positive cells (F4/80⁺CD11B⁺) indicate M2 macrophages⁽Reference Nguyen, Favelyukis and Nguyen³⁾. Fasting glucose, insulin, triacylglycerol (TAG) and non-esterified fatty acid (NEFA) concentrations were measured at weeks 0 and 6.

In the control group a larger population of the more active F4/80⁺CD11B⁺CD11C⁺ cells was present at week 6 compared with the IL-1R1^−/− group (P<0.05). After 6 weeks on a high fat diet TG and NEFA levels were significantly higher in both control and IL-1R1^−/− groups. Fasting glucose concentrations did not differ significantly over time in either control or IL-1R1^−/− groups. However, insulin concentrations in the control group at week 6 were significantly higher when compared with week 0 (P<0.05), pointing to insulin resistance. No significant differences in insulin levels in the IL-1R1^−/− mice were found.

These results imply that impairing IL-1 signalling decreases the macrophages ability to switch from an M2-polarization state to the pro-inflammatory M1-polarization state. This outcome reduces the infiltration of M1 macrophages into the adipose tissue, thereby lowering the pro-inflammatory response and attenuating the progression of insulin resistance. Further studies are continuing in order to determine the molecular mechanisms by which this process occurs.