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Distinct genetic profiles of Leishmania (Viannia) braziliensis associate with clinical variations in cutaneous-leishmaniasis patients from an endemic area in Brazil

Published online by Cambridge University Press:  12 March 2018

Patrícia Flávia Quaresma ,
Cristiana Ferreira Alves de Brito ,
Jeronimo Marteleto Nunes Rugani ,
Janaína de Moura Freire ,
Rodrigo de Paula Baptista ,
Elizabeth Castro Moreno ,
Raquel Carvalho Gontijo ,
Felipe Dutra Rego ,
Joaquim Edemilson Diniz  and
Maria Norma Melo
...Show all authors
Patrícia Flávia Quaresma*
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil
Cristiana Ferreira Alves de Brito
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
Jeronimo Marteleto Nunes Rugani
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
Janaína de Moura Freire
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
Rodrigo de Paula Baptista
Affiliation:
Center for Tropical & Emerging Global Diseases, University of Georgia, 30602-7394, Athens, Georgia, USA
Elizabeth Castro Moreno
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil
Raquel Carvalho Gontijo
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
Felipe Dutra Rego
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
Joaquim Edemilson Diniz
Affiliation:
Secretaria Municipal de Saúde, CEP: 39475-000, São João das Missões, Minas Gerais, Brazil
Maria Norma Melo
Affiliation:
Departamento de Parasitologia, ICB, Universidade Federal de Minas Gerais, CP: 486 – CEP: 31270-901, Belo Horizonte, Minas Gerais, Brazil
Célia Maria Ferreira Gontijo
Affiliation:
Instituto René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil
*
Author for correspondence: Patrícia Flávia Quaresma, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, CEP: 30190-002, Belo Horizonte, Minas Gerais, Brazil. Tel.: +55 31 3349 7755, fax +55 31 3291 7795 E-mail: patyquaresma@yahoo.com.br
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Abstract

American tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L. braziliensis, and 37 (95%) had a restriction profile corresponding to a variant pattern. A 1300-bp hsp70 gene fragment was sequenced to aid in parasite identification and a phylogenetic analysis was performed including 26 consensus sequences from the ATL patient's samples and comparing to other Leishmania and trypanosomatids species. The dendrogram allowed to observe a potential population structure of L. braziliensis complex in the studied region, emphasizing that the majority of clinical samples presented a variant genetic profile. Of interest, the L. braziliensis diversity was associated with different clinical manifestations whose parasites with hsp70 variant profile were associated with atypical lesions. The results may be helpful to improve the diagnosis, treatment and control measures of the ATL in endemic areas.

Keywords

Atypical lesionsBrazilcutaneous leishmaniasishsp70 geneLeishmania braziliensisparasite diversity
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1161 - 1169
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Copyright
Copyright © Cambridge University Press 2018 

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