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Improved Collagen Extraction by Modified Longin Method

Published online by Cambridge University Press:  18 July 2016

T A Brown ,
D E Nelson ,
J S Vogel  and
J R Southon
T A Brown
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
D E Nelson
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
J S Vogel*
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
J R Southon*
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
*
∗∗Also at: Tandem Accelerator Laboratory, McMaster University, Hamilton, Ontario L8S 4K1, Canada
∗∗Also at: Tandem Accelerator Laboratory, McMaster University, Hamilton, Ontario L8S 4K1, Canada
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Abstract

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A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.

Type
Research Article
Information
Radiocarbon , Volume 30 , Issue 2 , 1988 , pp. 171 - 177
Copyright
Copyright © The American Journal of Science 

References

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