2 results
Transcriptome profiling in depression with and without loss of appetite
- J. Pawlak, A. Szczepankiewicz, K. Bilska, P. Kapelski, P. Zakowicz, E. Paszynska, M. Dmitrzak-Weglarz
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- Journal:
- European Psychiatry / Volume 66 / Issue S1 / March 2023
- Published online by Cambridge University Press:
- 19 July 2023, p. S423
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Introduction
Depression has been described very comprehensively and is a highly prevalent mental condition. However, how its features develop and clinical course shape remains not fully understood.
ObjectivesThe study aimed to compare mRNA characteristics between specific symptoms and identification of differently expressed genes (DEGs) in patients with depression with specifiers such as loss of appetite, loss of weight, sleep disturbances and psychomotor retardation.
MethodsMaterial and method we used was transcriptome profiling of peripheral blood mononuclear cells in 30 patients diagnosed with depressive episode in course bipolar or unipolar affective disorder. The blood samples were drawn during acute depressive episode with at least moderate severity. The diagnosis and specific symptoms were described according to ICD-10 and DSM5 criteria using SCID-I, OPCRIT, and HDRS. Agilent microarrays were used for transcriptome profiling and GeneSpring software was applied. Minimal fold change 2 and significant p-value <0.05 were assumed. DAVID and KEGG databases were searched.
ResultsComparing depressed patients with and without decreased appetite or weight loss revealed 718 DEGs. When compared depressed patients with and without psychomotor retardation, 95 genes were up- or down regulated. In both comparisons DEGs were not identified as significant according to DAVID and KEGG database. When considering weight loss of more than 2 kg per month, 418 DEGs were identified. According to searched databases only one, characterized with phosphoserine phosphatase activity, was indicated as having a significant role in molecular functioning. The most numerous list of DEGs (n=855) was found when compared depressed patients with and without insomnia. Among these genes, several were indicated as significant for biological processes and cellular components: those linked with response to oxygen-compound, cytoplasmic and secretory vesicles and granules and circulatory system.
ConclusionsNumerous genes are differently expressed in depression with specific clinical features, such as appetite and sleeping disturbances, but their role in pathology remains unclear. One might expect that secretory and circulation activity is involved.
This research was funded by the National Science Center, Poland (Grant No: 2016/23/B/NZ5/02634) and supported by the Poznan University of Medical Sciences in Poland (Statute sources: 502-20-22196440).
Disclosure of InterestNone Declared
Anorexia nervosa in adolescence from oral health perspective
- E. Paszynska, A. Hernik, M. Tyszkiewicz-Nwafor, M. Dmitrzak-Weglarz, M. Roszak, A. Slopien
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- Journal:
- European Psychiatry / Volume 66 / Issue S1 / March 2023
- Published online by Cambridge University Press:
- 19 July 2023, pp. S524-S525
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Introduction
Management of patients with anorexia nervosa (AN) desires psychiatric/medical care. In average AN disease onset they represent a younger generation than 18 y.o. In this age typically children and adolescents are under regular dental care. Whether young AN patients should be included to intensive oral supervision may be still questionable. In literature little information on changes in oral cavity caused by AN were reported.
ObjectivesTherefore, the aim of the study was to evaluate caries incidence, tooth wear, gingival inflammation, and oral hygiene level among adolescent AN inpatients, highlighting the aspect of oral health manifestations in case-control study.
MethodsBased on clinically confirmed 130 AN restrictive subtype hospitalized female subjects (BMI <15 kg/m2, age 14.8±1.8), dental status has been examined regarding the occurrence of caries lesions using Decay Missing Filling Teeth (DMFT), erosive wear as Basic Erosive Wear Examination (BEWE), gingival condition as Bleeding on Probing (BOP) and plaque deposition as Plaque Control Record (PCR). The results were compared with age-matched 110 female controls (BMI 19.8±2.3 kg/m2, age 15.5±1.8, p=0.744) dentally caried in public University dental clinic (p<0.05) in the same time period.
ResultsAN patients compared with healthy adolescents were found to present higher incidence of oral-related complications according to dental status (DMFT 3.9±4.5 vs. 2.0±1.8, p=0.005), erosive tooth wear (BEWE 18.9% vs. 2.9%, p<0.001), less efficient in controlling plaque (PCR 43.8% vs. 13.7%, p<0.001) and gingival inflammation (20.0% vs. 3.9%, p<0.001). AN group, a significant correlation between BOP, BEWE, and duration of AN symptoms (p<0.05), similarly to the number of decayed teeth D, filled teeth F and PCR were detected (p<0.05).
ConclusionsAlthough the obtained results did not reveal any severe oral complications, AN diagnosis in adolescence may influence to numerous oral-related symptoms from dental caries, the onset of erosive tooth wear, failure in dental hygiene to be continuated as gingival inflammation. After AN diagnosis a regular preventive intervention should be performed during dental recall sessions. There is a need for professional oral hygiene/diet instructions combined with regular oral check up visits to avoid oral complications and disease progress. For clinical revelance an active collaboration between psychiatric and dental specialists is needed.
Disclosure of InterestNone Declared
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