Genome size (or C-value) in benthic polychaetes was studied from both experimental data and literature. Nuclear DNA content variation was analysed as related to both phylogenetic relationships of various families, general life habit of the species (interstitial vs macrobenthic), diploid chromosome number (2n), and mean body size (length). Experimental data on genome size were obtained for 43 lower taxa (genera and species) by means of microdensitometric analysis of the amount of Fuelgen-stained haploid DNA in interphase nuclei. These data, added to those available in the literature (36 taxa studied by Conner et al., 1972) gave a total of 79 taxa (genera and species), representing 30 families and 14 orders. Twenty-one of the species studied belonged exclusively to interstitial (=meiofaunal) families or genera.