Fall, spring, or late-summer applications of glyphosate, paraquat, sethoxydim, and HOE-39866 were investigated in field trials for no-tillage renovation of endophyte fungus-infected tall fescue pastures. Only spring or late-summer applications of glyphosate at 1.68 and 2.52 kg ae ha−1 consistently provided greater than 80% visual tall fescue control. However, none of the herbicide treatments significantly reduced final percent endophyte fungus infection levels after the pasture was reestablished. High final infection levels were due to regrowth from underground rhizomes of old tall fescue sod that survived the herbicide treatments.