Greenhouse and field experiments were conducted to determine effects of light, water, and chlorsulfuron on growth of Canada thistle. In the greenhouse, shoot and root dry weight, leaf area, and number of inflorescences decreased as light and water levels decreased. In the field, shoot and root dry weight, leaf area, and number of Canada thistle inflorescences were positively correlated with light intensity from 1832 to 30 μmol m−2 s−1 photosynthetic photon flux density (PPFD). The greatest effect of light was on inflorescence production which was eliminated at 30 μmol m−2 s−1 (PPFD). The combined effect of water stress and chlorsulfuron decreased root and shoot growth but did not eliminate it.