Matings of Drosophila pseudoobscura were observed in Elens-Wattiaux chambers, using individuals of three karyotypes: AR/AR, AR/CH and CH/CH. In each chamber two karyotypes were represented, with frequencies 10:10, 2:18 or 18:2. The males of the heterokaryotype AR/CH tend to have an advantage in mating compared to the homokaryotypes, provided that both kinds of males are equally frequent, i.e. the ratio 10:10. This advantage is further increased when the heterokaryotype is a minority, the ratio 2:18. When the homokaryotype, AR/AR or CH/CH, is a minority (18:2) it is equally or more successful than the heterokaryotype in securing mates. Among females, the mating success is independent of frequency, or the minority females have sometimes only a slight advantage.