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Pseudomonas fluorescens and Pseudomonas putida from refrigerated raw milk: genetic diversity and lipoproteolytic activity
- Rosana De Longhi, Selma de Souza Correia, Samera Rafaela Bruzaroski, Regina Celia Poli-Frederico, Rafael Fagnani, Elsa Helena Walter de Santana
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- Journal:
- Journal of Dairy Research / Volume 89 / Issue 1 / February 2022
- Published online by Cambridge University Press:
- 11 February 2022, pp. 86-89
- Print publication:
- February 2022
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In this research communication the genetic diversity of Pseudomonas fluorescens (n = 67) and Pseudomonas putida (n = 44) isolated from refrigerated raw milk from bulk tank trucks were verified. The relationship between the genetic profile of the isolates and their lipoproteolytic potential was evaluated using skim milk agar and tributyrin agar (21°C/72 h). The lipoproteolytic potential (low or high), evaluated by the diameter of the halos (cm), was correlated with the number of milk producing properties that contributed to each sample (one sample = one bulk tank truck; 8−80 producers/sample) and the distance between the dairy properties and the processing plant (21−370 km). P. fluorescens was confirmed in all samples, while P. putida in 60% samples. For both species, two clusters (I and II) were observed, and the first one showed lower genotypic diversity and the presence of isolates with 100% similarity. P. fluorescens isolates presenting at least 70% similarity were 83.9% in Cluster I (n = 31) and 44.4% in Cluster II. In both clusters (I and II) observed in the P. fluorescens dendrogram, the occurrence of high proteolytic and lipolytic potential were equivalent. The higher the number of farms per milk sample, the greater the lipoproteolytic intensity of P. fluorescens isolates. In relation to P. putida isolates, 74% presented at least 50% similarity in Cluster I (n = 27) and only 35% in Cluster II (n = 17). The occurrence of high proteolysis linked to P. putida was proportional between both Clusters, but the occurrence of high lipolysis was greater in Cluster II. No significant association was detected between P. putida isolates and the variables studied. The results indicate the circulation of P. putida and P. fluorescens with 100% similarity in different milk producing regions. The level of genetic diversity was related only to the lipolytic capacity of P. putida.
Remote Mock OSCE (ReMO): The “new normal”?
- Rachel Brown, Elsa Brew-Girard, Stephen De Souza
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- Journal:
- BJPsych Open / Volume 7 / Issue S1 / June 2021
- Published online by Cambridge University Press:
- 18 June 2021, p. S128
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- Article
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- Open access
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Aims
In March 2020, COVID-19 and its associated restrictions forced a halt to in-person teaching and assessment. To try and mitigate this disruption, the psychiatry undergraduate teaching faculty developed a knowledge based remote curriculum. However, it became clear that our students sorely missed clinical and consultation experience. Prior to the pandemic we had delivered a mock Objective Structured Clinical Examination (OSCE) to those undertaking their psychiatry block. In Somerset Academy, we wanted to deliver a distanced alternative: the remote mock OSCE (ReMO). We hoped to demonstrate this would be a feasible and valuable learning experience.
MethodIn keeping with other OSCEs, ReMO had active stations (4) and a rest station. Four simultaneous Skype meetings were set up as clinical stations, each with an examiner and actor. To test the technology, students and facilitators were emailed links to each meeting in advance, and invited to sign in. Students were given individualised timings to rotate between stations. Stations involved history taking, risk assessment, and management discussions of common psychiatric presentations.
The students then rotated again, receiving personalised feedback about their performance, enabling immediate reflection and consideration of areas for development. This was followed up with written feedback, using examiner completed mark schemes.
ResultAfter ReMO we invited feedback from medical students and facilitators. 7 out of the 8 medical students that participated completed a post-ReMO survey. 100% of students found ReMO “useful”, with 71% (5/7) rating it an “extremely valuable” experience and 29% (2/7) rating it “fairly valuable”. Students felt it was well organised, realistic, and increased their confidence in remote consultations and OSCE practice. 6 out of 8 facilitators completed feedback on ReMO. 100% felt that ReMO was reproducible and 83% (5/6) rated it as “fairly realistic” when compared to the face-to-face standard.
ConclusionFirstly, ReMO was feasible. However, it was logistically difficult, requiring extensive organisation to ensure this relatively small group were in the right place at the right time. In future, we would consider alternative platforms such as Zoom, or specific consultation software, such as Attend Anywhere, to reduce the logistics burden and utilise features such as ‘breakout rooms’. We would recommend an allocated co-ordinator to troubleshoot any problems in real time via a group messaging service.
In conclusion, ReMO is achievable and a valuable student learning experience. Since the pilot it has become an integral part of our curriculum. We recommend that all undergraduate Psychiatry faculties consider adding it to their programme.
Management challenges for the fastest growing marine shipping sector in Arctic Canada: pleasure crafts
- Margaret Johnston, Jackie Dawson, Elsa De Souza, Emma J. Stewart
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- Journal:
- Polar Record / Volume 53 / Issue 1 / January 2017
- Published online by Cambridge University Press:
- 29 September 2016, pp. 67-78
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Changing environmental conditions in the Canadian Arctic are associated with an increase in marine tourism. A substantial decline in the extent of ice coverage in the summer season has resulted in greater accessibility for all categories of ships, and the tourism sector has been quick to respond to new opportunities. This increase in vessel traffic has raised significant issues for management, and particular concerns about the pleasure craft (non-commercial tourism) sector. This paper reports on research aimed at identifying change in the pleasure craft sector in Canadian Arctic waters since 1990; exploring management concerns held by stakeholders regarding changes in the sector; and, providing recommendations for government stakeholders. The paper is based on material gathered through an examination of existing data sources and stakeholder interviews (n = 22). Analysis was aimed at understanding the rapid development of the sector and potential management strategies, including research needs. Analysis reveals a dramatic increase in annual vessel numbers, particularly from 2010 onwards. Management concerns of interviewees relate to implications of this growth in four areas: visitor behaviour; services, facilities and infrastructure; control; and, planning and development. The paper concludes by describing recommendations in the areas of research needs, regulation, and strategic development.