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TRIBES AND CLADES WITHIN APIACEAE SUBFAMILY APIOIDEAE: THE CONTRIBUTION OF MOLECULAR DATA
- S. R. DOWNIE, G. M. PLUNKETT, M. F. WATSON, K. SPALIK, D. S. KATZ-DOWNIE, C. M. VALIEJO-ROMAN, E. I. TERENTIEVA, A. V. TROITSKY, B.-Y. LEE, J. LAHHAM, A. EL-OQLAH
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- Journal:
- Edinburgh Journal of Botany / Volume 58 / Issue 2 / June 2001
- Published online by Cambridge University Press:
- 23 October 2001, pp. 301-330
- Print publication:
- June 2001
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Phylogenetic analyses of chloroplast gene (rbcL, matK), intron (rpl16, rps16, rpoC1) and nuclear ribosomal DNA internal transcribed spacer (ITS) sequences and chloroplast DNA restriction sites, with supplementary data from variation in size of the chloroplast genome inverted repeat, have been used to elucidate major clades within Apiaceae (Umbelliferae) subfamily Apioideae Drude. This paper summarizes the results of previously published molecular cladistic analyses and presents a provisional classification of the subfamily based on taxonomic congruence among the data sets. Ten tribes (Aciphylleae M. F. Watson & S. R. Downie, Bupleureae Spreng., Careae Baill., Echinophoreae Benth., Heteromorpheae M. F. Watson & S. R. Downie, Oenantheae Dumort., Pleurospermeae M. F. Watson & S. R. Downie, Pyramidoptereae Boiss., Scandiceae Spreng. and Smyrnieae Spreng.) are erected or confirmed as monophyletic, with Scandiceae comprising subtribes Daucinae Dumort., Scandicinae Tausch and Torilidinae Dumort. Seven additional clades are also recognized but have yet to be treated formally, and at least 23 genera examined to date are of dubious tribal or clade placement. The utility of these different molecular markers for phylogenetic inference in Apioideae is compared based on maximum parsimony analyses of subsets of previously published molecular data sets. Of the six loci sequenced, the ITS region is seen to be evolving most rapidly and rbcL is the most conservative. Intermediate in rate of evolution are matK and the three chloroplast introns; with rpl16 and rps16 evolving slightly faster than matK or rpoC1. The analysis of restriction sites, however, provided 2–4 times more parsimony informative characters than any single DNA locus sequenced, with estimates of divergence just slightly lower than that of the ITS region. The trees obtained from separate analyses of these reduced data sets are consistent with regard to the major clades inferred and the relationships among them. Similar phylogenies are obtained by combining data or combining trees, representing the supermatrix and supertree approaches to phylogenetic analysis, respectively. The inferred relationship among the tribes and informally recognized major clades within Apioideae is presented.
Morphological diversity of ground beetles (Coleoptera: Carabidae) in Scottish agricultural land
- I. Ribera, D. I. McCracken, G. N. Foster, I. S. Downie, V. J. Abernethy
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- Journal:
- Journal of Zoology / Volume 247 / Issue 1 / January 1999
- Published online by Cambridge University Press:
- 01 January 1999, pp. 1-18
- Print publication:
- January 1999
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The morphospace defined by 87 species of ground beetle (Coleoptera: Carabidae) of Scottish non-forested habitats is described with multivariate methods, using 13 linear quantitative measurements of the body, hind legs, eyes and antennae, plus five qualitative characters concerned with body shape, colour, wing development, and pubescence. Relationships between pairs of variables are studied with phylogenetic independent contrasts, using two different taxonomic classifications as an approximation to the phylogeny of the group. The first ordination axis of the morphospace was found mainly to reflect the positive correlation between length of the antennae and length of the hind legs, the second to reflect the width of the head, diameter of the eye and pronotum height, and the third the width of the pronotum and elytra, length of the metatrochanter and width of the metafemur. The principal relationships involving qualitative characters were between colour of the body and legs and shape of the pronotum with ordination axes, wing development with width of the elytra, and pubescence with colour of the legs. Most correlations between quantitative variables, in particular those most influencing the ordination axes of the morphospace, remained significant when measured with phylogenetic independent contrasts using both classifications. Independent contrasts comparing qualitative with quantitative variables or ordination axes were only significant for the colour of the body with the second axis for both classifications used, and length of the antennae with colour of the body and shape of the pronotum for only one of the classifications. The main morphological trends within the morphospace defined are related with published information on their performance, in particular running speed and pushing abilities, following previous work on the functional morphology of the group. The morphospace defined by the species studied is a fundamental tool that will allow further investigations on the relationships between their morphology and life traits, as well as on the relationships of the functional diversity thus characterized with environmental correlates.