A recent study published in Oryx proposed that the extinct Javan tiger Panthera tigris sondaica may still survive on the Island of Java, Indonesia, based on mitochondrial DNA analysis of a single hair sample collected from a location where a tiger was reportedly encountered. However, upon reanalysing the genetic data presented in that study, we conclude that there is little support for this claim. The sequences of the putative tiger hair and Javan tiger museum specimens generated are not from tiger cytoplasmic mitochondrial DNA but more likely the nuclear pseudogene copies of mitochondrial DNA. In addition, the number of mismatches between the two Javan tiger sequences is unusually high for homologous sequences that are both from tigers, suggesting potential issues with data reliability. The paper provides insufficient details on quality control measures, making it impossible to rule out the possibility that errors were introduced during the analysis. Consequently, it is inappropriate to use the sequences presented in that study to infer the existence of the Javan tiger.

Long-duration gamma-ray bursts (GRBs) are considered to be due to the death of massive stars. Therefore, GRBs are closely associated with the star formation of host galaxies. Since GRBs can be detected at cosmological distances, they are expected to be probes of the star formation history of the Universe. In order to determine the use of GRBs, it is essential to understand the star formation of their hosts. Multi-wavelength observations have shown that the star formation rates (SFRs) of GRB hosts derived from submillimeter/radio observations are generally higher than those from optical/UV observations (Berger et al. 2003). This implies that GRB hosts have a large amount of molecular gas and massive star formation obscured by dust. In order to solve this problem, it is necessary to derive the SFRs in a method which is independent of existing methods and not affected by dust extinction.

We observed 12CO (J = 3–2) line emission from the host galaxy of GRB 980425 using the Atacama Submillimeter Telescope Experiment (ASTE). Five points were observed covering the entire region of the galaxy, and we find possible emission features (S/N ~ 3 σ) at the velocity range corresponding to the redshift of the galaxy. By combining all spectra of five points, we obtain a global spectrum with a ~4 σ emission feature. If the features are real, this is the first detection of CO among GRB hosts. We derive the total gas mass of M(H2)=7 ± 2× 108M⊙ assuming a CO-to-H2 conversion factor of αCO = 8.0M⊙ (K km s−1 pc2)−1, which is deduced using the correlation between the αCO and the metallicity. The dynamical mass is calculated to be Mdyn=2× 1010M⊙, and M(H2)/Mdyn~3% is consistent with those of nearby dwarfs and normal spirals. The derived SFR is 0.5 ± 0.1 M⊙ yr−1 based on the Schmidt law. This SFR agrees with the results of previous Hα observations, suggesting that there is no significant obscured star formation in this host galaxy. This result implies that there is a variety of GRB hosts in terms of the presence of obscured star formation.

A recent estimate suggests that the Scottish wildcat may be critically endangered. Nevertheless, there is still no uncontroversial method for diagnosing the Scottish wildcat. We analysed morphological differences between wild-living cats in Scotland on the basis of 20pelage characters, scoring from 1 (domestic cat) to 3 (wildcat), in combination with 40skull parameters and intestinal length. A cluster analysis, based on Principal Components derived from the scores for pelage characters, showed that the wild-living cats fell into three main groups without any a priori classification. Each group corresponds well to the traditional characteristics of wildcats, hybrids and domestic cats, respectively, and the former two each show higher levels of morphological homogeneity compared with the third group. The three groups are most significantly differentiated by seven pelage characters: (1) extent of dorsal stripe, (2) shape of tail tip, (3) distinctness of tail bands, (4) presence/absence of broken stripes and (5) spots, on flanks and hindquarters, (6) shape and number of stripes on nape and (7) on the shoulders. Most Group-1 cats (75.6%, n=74), but none of the other two groups, score more than 2 for all seven characters. All Group-3 cats (n=35) and some Group-2 cats (19.2%, n=26), but no Group-1 cats, scored 1 for one or more of the seven characters. We propose that Group-1, which is the furthest from the domestic cat in all criteria, should be used to define the Scottish wildcat. However, in practice, if a wild-living cat does not score 1 for any of the seven characters it should be treated as a wildcat in the field. These definitions provide a simple way of diagnosing a Scottish wildcat scientifically, as well as practically, which will effectively facilitate conservation action and the enforcement of protective legislation.

The natural morphological variation in the wildcat, Felis silvestris, and morphological changes possibly caused by introgressive hybridisation with the domestic cat, F. catus, were examined, based on up to 39 variables concerning cranial morphology. The samples of wild-living cats originated from Scotland and southern Africa and consisted of both classical wildcat and other pelage types. Principal component and cluster analyses suggested that introgressive hybridisation occurred in both areas, with the consequence that the characteristics of local wildcat populations had been altered in terms of the frequencies of occurrence of certain characters, especially those concerning cranial capacity. In both regions the clustering patterns of wild-living cats can be interpreted as containing four main groups. One of these consisted mainly of ‘non-wildcats’ and groups furthest from the ‘non-wild’ cluster consisted of the highest proportion of ‘wildcats’ (c. 80%). We propose that where a population is heavily introgressed, the only feasible way to define a wildcat is on the basis of inter-correlated features and conservationists must take a population-based approach to assess the extent of introgression. This approach may provide an operational standard for assessing the impact of hybridisation between wildcats and domestic cats throughout the species' range; it suggests that the Scottish wildcats may be critically endangered.

The evolutionary history of the lion Panthera leo began in Pliocene east Africa, as open habitats expanded towards the end of the Cenozoic. During the middle–late Pleistocene, lions spread to most parts of Eurasia, North America, and may have eventually reached as far south as Peru. Lions probably evolved group-living behaviour before they expanded out of Africa, and this trait is likely to have prevailed in subsequent populations. The first lions were presumed to have been maneless, and maneless forms seem to have persisted in Europe, and possibly the New World, until around 10 000 years ago. The maned form may have appeared c. 320 000–190 000 years ago, and may have had a selective advantage that enabled it to expand to replace the range of earlier maneless forms throughout Africa and western Eurasia by historic times: ‘latest wave hypothesis’.