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Goat’s αS1-casein polymorphism affects gene expression profile of lactating mammary gland

Published online by Cambridge University Press:  01 April 2008

S. Ollier ,
S. Chauvet ,
P. Martin ,
Y. Chilliard  and
C. Leroux
S. Ollier
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
S. Chauvet
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
P. Martin
Affiliation:
INRA, UR1196 Génomique et Physiologie de la Lactation, Domaine de Vilvert, F-78352 Jouy-en-Josas, France
Y. Chilliard
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
C. Leroux*
Affiliation:
INRA, UR1213 Herbivores, Site de Theix, F-63122 Saint-Genès-Champanelle, France
*
E-mail: cleroux@clermont.inra.fr
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Abstract

Goat’s αS1-casein (CSN1S1) polymorphism has a significant effect on milk protein and lipid composition, which affects the nutritional quality and technological properties of milk. Moreover, this polymorphism has a large impact on the morphology of mammary epithelial cells. To explore the metabolic pathways modulated in relation to this polymorphism, we compared the mammary gene expression profiles of two groups of lactating goats carrying either two reference or two defective alleles, using a bovine oligonucleotide microarray representing 8379 genes. We identified 41 differentially expressed genes between the two genotype groups. In particular, we showed a downregulation of two key lipogenic genes encoding fatty acid synthase and glycerol-3-phosphate acyltransferase in agreement with the low fat concentration associated with CSN1S1 deficiency. In addition, this study highlights changes in the expression level of several genes known to influence membrane fluidity, cell–cell interaction or chromatin organization. Our results open up new fields of investigation on structural modifications associated with CSN1S1 deficiency that could affect mammary gland function.

Keywords

CSN1S1genetic polymorphismmammary transcriptomemicroarraycell organization
Type
Full Paper
Information
animal , Volume 2 , Issue 4 , April 2008 , pp. 566 - 573
Copyright
Copyright © The Animal Consortium 2008

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References

Association of Official Analytical Chemists 1997. Official Methods of Analysis, 16th edition. AOAC International, Gaithersburg, MD, USA.Google Scholar
Barbieri, ME, Manfredi, E, Elsen, J-M, Ricordeau, G, Bouillon, J, Grosclaude, F, Mahé, M-F, Bibé, B 1995. Influence du locus de la caséine alpha-s1 sur les performances laitières et les paramètres génétiques des chèvres de race Alpine. Genetics Selection Evolution 27, 437–450.CrossRefGoogle Scholar
Bernard, L, Rouel, J, Leroux, C, Ferlay, A, Faulconnier, Y, Legrand, P, Chilliard, Y 2005. Mammary lipid metabolism and milk fatty acid secretion in Alpine goats fed vegetable lipids. Journal of Dairy Science 88, 1478–1489.CrossRefGoogle ScholarPubMed
Bonnet, M, Leroux, C, Faulconnier, Y, Hocquette, JF, Bocquier, F, Martin, P, Chilliard, Y 2000. Lipoprotein lipase activity and mRNA are up-regulated by refeeding in adipose tissue and cardiac muscle of sheep. Journal of Nutrition 130, 749–756.Google Scholar
Burgers, PM 1998. Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107, 218–227.CrossRefGoogle ScholarPubMed
Carraway, KL, Ramsauer, VP, Carraway, CA 2005. Glycoprotein contributions to mammary gland and mammary tumor structure and function: roles of adherens junctions, ErbBs and membrane MUCs. Journal of Cellular Biochemistry 96, 914–926.Google Scholar
Chanat, E, Martin, P, Ollivier-Bousquet, M 1999. Alpha(S1)-casein is required for the efficient transport of beta- and kappa-casein from the endoplasmic reticulum to the Golgi apparatus of mammary epithelial cells. Journal of Cell Science 112, 3399–3412.CrossRefGoogle Scholar
Chilliard, Y, Rouel, J, Leroux, C 2006. Goat’s alpha-s1 casein genotype influences its milk fatty acid composition and delta-9 desaturation ratios. Animal Feed Science and Technology 131, 474–487.CrossRefGoogle Scholar
Clark, S, Sherbon, JW 2000. AlphaS1-casein, milk composition and coagulation properties of goat milk. Small Ruminant Research 38, 123–134.Google Scholar
Coleman, RA, Lewin, TM, Muoio, DM 2000. Physiological and nutritional regulation of enzymes of triacylglycerol synthesis. Annual Review of Nutrition 20, 77–103.Google Scholar
JrDennis, G, Sherman, BT, Hosack, DA, Yang, J, Gao, W, Lane, HC, Lempicki, RA 2003. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biology 4, P3.CrossRefGoogle ScholarPubMed
Dyson, N 1998. The regulation of E2F by pRB-family proteins. Genes and Development 12, 2245–2262.Google Scholar
Frick, C, Atanasov, AG, Arnold, P, Ozols, J, Odermatt, A 2004. Appropriate function of 11beta-hydroxysteroid dehydrogenase type 1 in the endoplasmic reticulum lumen is dependent on its N-terminal region sharing similar topological determinants with 50-kDa esterase. Journal of Biological Chemistry 279, 31131–31138.CrossRefGoogle ScholarPubMed
Gorska, MM, Stafford, SJ, Cen, O, Sur, S, Alam, R 2004. Unc119, a novel activator of Lck/Fyn, is essential for T cell activation. Journal of Experimental Medicine 199, 369–379.CrossRefGoogle ScholarPubMed
Grosclaude, F, Martin, P, Ricordeau, G, Remeuf, F, Vassal, L, Bouillon, J 1994. Du gène au fromage: le polymorphisme de la caséine alpha s1 caprine, ses effets, son évolution. Productions Animales 7, 3–19.Google Scholar
Heldin, CH, Miyazono, K, ten Dijke, P 1997. TGF-beta signalling from cell membrane to nucleus through SMAD proteins. Nature 390, 465–471.Google Scholar
Jones, MH, Hamana, N, Nezu, J, Shimane, M 2000. A novel family of bromodomain genes. Genomics 63, 40–45.Google Scholar
Lamberet, G, Degas, C, Delacroix-Buchet, A, Vassal, L 1996. Influence de caractères liés aux allèles A et F de la caséine αs1 caprine sur la flaveur chèvre: fabrications fromagères avec échange de protéines et de matières grasses. Le Lait 76, 349–361.CrossRefGoogle Scholar
Leroux, C, Mazure, N, Martin, P 1992. Mutations away from splice site recognition sequences might cis-modulate alternative splicing of goat alpha s1-casein transcripts. Structural organization of the relevant gene. Journal of Biological Chemistry 267, 6147–6157.CrossRefGoogle ScholarPubMed
Leroux, C, Le Provost, F, Petit, E, Bernard, L, Chilliard, Y, Martin, P 2003. Real-time RT-PCR and cDNA macroarray to study the impact of the genetic polymorphism at the alphas1-casein locus on the expression of genes in the goat mammary gland during lactation. Reproduction, Nutrition, Development 43, 459–469.CrossRefGoogle Scholar
Li, Y, DeFatta, R, Anthony, C, Sunavala, G, De Benedetti, A 2001. A translationally regulated Tousled kinase phosphorylates histone H3 and confers radioresistance when overexpressed. Oncogene 20, 726–738.CrossRefGoogle ScholarPubMed
Moioli, B, D’Andrea, M, Pilla, F 2007. Candidate genes affecting sheep and goat milk quality. Small Ruminant Research 68, 179–192.CrossRefGoogle Scholar
Neveu, C, Riaublanc, A, Miranda, G, Chich, JF, Martin, P 2002. Is the apocrine milk secretion process observed in the goat species rooted in the perturbation of the intracellular transport mechanism induced by defective alleles at the alpha(s1)-Cn locus? Reproduction, Nutrition, Development 42, 163–172.CrossRefGoogle ScholarPubMed
Ntambi, JM 1999. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lipid Research 40, 1549–1558.Google Scholar
Ollier, S, Robert-Granie, C, Bernard, L, Chilliard, Y, Leroux, C 2007. Mammary transcriptome analysis of food-deprived lactating goats highlights genes involved in milk secretion and programmed cell death. Journal of Nutrition 137, 560–567.CrossRefGoogle ScholarPubMed
Pascall, JC, Brown, KD 1998. Characterization of a mammalian cDNA encoding a protein with high sequence similarity to the Drosophila regulatory protein Rhomboid. FEBS Letters 429, 337–340.Google Scholar
Perez, MJ, Leroux, C, Bonastre, AS, Martin, P 1994. Occurrence of a LINE sequence in the 3′ UTR of the goat alpha s1-casein E-encoding allele associated with reduced protein synthesis level. Gene 147, 179–187.Google Scholar
Piedra, J, Miravet, S, Castano, J, Palmer, HG, Heisterkamp, N, Garcia de Herreros, A, Dunach, M 2003. p120 Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin Interaction. Molecular and Cellular Biology 23, 2287–2297.CrossRefGoogle ScholarPubMed
Remeuf, F 1993. Influence du polymorphisme génétique de la caséine alpha-s1 caprine sur les caractéristiques physico-chimiques et technologiques du lait. Le Lait 73, 549–557.CrossRefGoogle Scholar
Samuel, W, Nagineni, CN, Kutty, RK, Parks, WT, Gordon, JS, Prouty, SM, Hooks, JJ, Wiggert, B 2002. Transforming growth factor-β regulates stearoyl coenzyme A desaturase expression through a Smad signaling pathway. Journal of Biological Chemistry 277, 59–66.Google Scholar
Schibler, L, Vaiman, D, Oustry, A, Giraud-Delville, C, Cribiu, EP 1998. Comparative gene mapping: a fine-scale survey of chromosome rearrangements between ruminants and humans. Genome Research 8, 901–915.CrossRefGoogle ScholarPubMed
Sillje, HH, Nigg, EA 2001. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases. Current Biology 11, 1068–1073.CrossRefGoogle ScholarPubMed
Sillje, HH, Takahashi, K, Tanaka, K, Van Houwe, G, Nigg, EA 1999. Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. EMBO Journal 18, 5691–5702.CrossRefGoogle ScholarPubMed
Smith, S 1994. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB Journal 8, 1248–1259.CrossRefGoogle ScholarPubMed
Takeuchi, M, Kuramochi, S, Fusaki, N, Nada, S, Kawamura-Tsuzuku, J, Matsuda, S, Semba, K, Toyoshima, K, Okada, M, Yamamoto, T 1993. Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. Journal of Biological Chemistry 268, 27413–27419.CrossRefGoogle ScholarPubMed