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Degradation of terpenes and terpenoids from Mediterranean rangelands by mixed rumen bacteria in vitro

Published online by Cambridge University Press:  10 October 2011

M. Malecky ,
H. Albarello  and
L. P. Broudiscou
M. Malecky
Affiliation:
INRA, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France AgroParisTech, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
H. Albarello*
Affiliation:
INRA, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France AgroParisTech, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
L. P. Broudiscou*
Affiliation:
INRA, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France AgroParisTech, UMR 791 Modélisation Systémique Appliquée aux Ruminants, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
*
†E-mail: laurent.broudiscou@agroparistech.fr
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Abstract

This in vitro study aimed at estimating the disappearance rates of 14 terpenes and terpenoids after 24-h incubation with mixed bacteria from caprine rumens. These compounds comprised nine monoterpene hydrocarbons (δ-3-carene, p-cymene, β-myrcene, (E)- and (Z)-β-ocimene, α-phellandrene, α-terpinene, γ-terpinene and α-terpinolene), four oxygenated monoterpenes ((E)- and (Z)-linalool oxide, 4-terpinenol, α + γ terpineol) and one sesquiterpene hydrocarbon (β-cedrene). They were individually exposed to goat rumen microflora for 24 h in 70 ml culture tubes at an input level of 0.5 ml/l. Terpenoids were the least degraded, 100% of (E)-linalool oxide, 95% of (Z)-linalool oxide, 91% of 4-terpinenol and 75% of terpineol remained intact after 24-h incubation. In contrast, α-terpinolene concentration in fermentation broth extracts was below quantification limit, thus indicating an extensive, if not complete, degradation by rumen bacteria. Only 2% of the initial amounts of α-phellandrene were recovered. The other monoterpenes and β-cedrene were partly degraded, with losses ranging from 67% for δ-3-carene to 90% for (E)-β-ocimene. The corresponding rates of disappearance were between 2.67 and 4.08 μmol/ml inoculum per day.

Keywords

monoterpenemonoterpenoidsesquiterpenerumen bacteriadegradation
Type
Full Paper
Information
animal , Volume 6 , Issue 4 , April 2012 , pp. 612 - 616
Copyright
Copyright © The Animal Consortium 2011

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