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l-Leucine supplementation reduces growth performance accompanied by changed profiles of plasma amino acids and expression of jejunal amino acid transporters in breast-fed intra-uterine growth-retarded piglets

Published online by Cambridge University Press:  01 September 2022

Yun Ji ,
Yuli Sun ,
Ning Liu ,
Hai Jia ,
Zhaolai Dai ,
Ying Yang  and
Zhenlong Wu Open the ORCID record for Zhenlong Wu [Opens in a new window]
Yun Ji
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Yuli Sun
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Ning Liu
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Hai Jia
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Zhaolai Dai
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Ying Yang
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
Zhenlong Wu*
Affiliation:
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China
*
*Corresponding author: Dr Z. Wu, fax +86 10 62731003, email wuzhenlong@cau.edu.cn
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Abstract

Previously, we provided an evidence that l-Leucine supplementation facilitates growth performance in suckling piglets with normal birth weight. However, it remains hitherto obscure weather breast-fed piglets displaying intra-uterine growth restriction (IUGR) show a similar effect in response to l-Leucine provision. In this study, 7-d-old sow-reared IUGR piglets were orally administrated with l-Leucine (0, 0·7, 1·4 or 2·1 g/kg BW) twice daily for 2 weeks. Increasing leucine levels hampered the growth performance of suckling IUGR piglets. The average daily gain of IUGR piglets was significantly reduced in 1·4 g/kg BW and 2·1 g/kg BW l-Leucine supplementation groups (P < 0·05). Except for ornithine and glutamine, the plasma concentrations of other amino acids were abated as l-Leucine levels increased (P < 0·05). Leucine supplementation led to reduction in the levels of urea, blood ammonia, blood glucose, TAG and total cholesterol, as well as an elevation in the level of LDL-cholesterol in suckling IUGR piglets (P < 0·05). In addition, 1·4 g/kg BW of l-Leucine enhanced the mRNA expression of ATB 0,+, whereas decreased the mRNA abundances of CAT1, y + LAT1, ASCT2 and b 0,+AT in the jejunum (P < 0·05). Concomitantly, the jejunum of IUGR piglets in l-Leucine group contains more ATB0,+ and less SNAT2 protein than in the control (P < 0·05). Collectively, l-Leucine supplementation impairs growth performance in breast-fed IUGR piglets, which may be associated with depressed nutritional conditions and alterations in the uptake of amino acids and the expression of amino acid transporters in the small intestine.

Keywords

LeucineIntra-uterine growth restrictionAmino acidsPigletsTransporter
Type
Research Article
Information
British Journal of Nutrition , Volume 129 , Issue 12 , 28 June 2023 , pp. 2025 - 2035
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Copyright
© The Author(s), 2022. Published by Cambridge University Press on behalf of The Nutrition Society

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Footnotes

†

These authors contributed equally to this work.

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