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Autosomal inheritance of alphamethrin, a synthetic pyrethroid, resistance in Anopheles stephensi – Liston, a malaria mosquito

Published online by Cambridge University Press:  22 March 2013

T.P.N. Hari Prasad  and
N.J. Shetty
T.P.N. Hari Prasad*
Affiliation:
Centre for Applied Genetics, Jnana Bharathi, Bangalore University, Bangalore 560 056, India
N.J. Shetty
Affiliation:
Centre for Applied Genetics, Jnana Bharathi, Bangalore University, Bangalore 560 056, India Janardhana Foundation, Nagadevanahalli, Jnana Bharathi Post, Bangalore 560 056, India
*
*Author for correspondence Phone: +91 80 22961301 Fax: +91 80 23212318 E-mail: hariprasad.tpn@gmail.com
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Abstract

Anopheles stephensi – Liston (Culicidae: Diptera) is an important urban malarial vector in the Indian sub-continent, accounting for about 15% of the total annual malaria incidence. Chemical control represents a key strategy in the management of this insect vector. However, owing to erratic and continuous application of insecticides, resistance has become a common phenomenon among them and their control has become an uphill task. The genetics of alphamethrin, a synthetic pyrethroid resistance was studied to determine its mode of inheritance. The late third instar larvae were selectively inbred for 27 and ten generations to synthesize homozygous resistant (R) and susceptible (S) stocks, respectively, to the diagnostic dose of 0.12 mg l−1. The log-dosage probit mortality relationships and degree of dominance (D) were calculated. Resistance was observed in both sexes, the dosage-mortality (d-m) line of F1 was towards the resistant parent and the ‘D’ value was found to be 0.8 indicating alphamethrin resistant (amr) gene to be autosomal and incompletely dominant. The d-m lines of F2/backcross exhibited a clear plateau of mortality across a range of doses indicating monogenic resistance. The null hypothesis for monogenic resistance was tested from mortality data of backcross progeny compared with theoretical expectations using the χ2 test and was found to be non-significant. Understanding genetics of insecticide resistance is significant in prediction and management of resistant insects. The amr genes can be used as genetic marker in A. stephensi, which can be used in several applications in conducting basic and applied genetic research.

Keywords

Anopheles stephensialphamethrinresistancemonofactorialautosomalincompletely dominant
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 5 , October 2013 , pp. 547 - 554
Copyright
Copyright © Cambridge University Press 2013 

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