Skip to main content
×
Home

Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep

  • T. H. Terrill (a1), G. C. Waghorn (a2), D. J. Woolley (a3), W. C. Mcnabb (a1) and T. N. Barry (a1)...
Abstract

Three experiments were conducted to determine the fate of condensed tannins (CT) during digestion in sheep. CT were measured as extractable, protein-bound and fibre-bound fractions using the butanol-HCI procedure. In Expt 1, purified CT were added to digesta from different parts of the digestive tract obtained from a pasture-fed sheep. Recoveries of CT after 0 and 4 h of anaerobic incubation at 39° averaged: rumen 78.9 and 57.5 %; abomasum 50.9 and 49.0 %; duodenum 64.4 and 46.0 % and ileum 43.4 and 38.8%. In Expt 2, [14C]CT was given per abomasum over a 6.5 h period at 15 min intervals to a sheep previously fed on Lotus pedunculatus (which contains CT). The sheep was killed at the end of the period and 92.4% of the label was recovered. Virtually all of the label was in the digesta, and none was detected in the blood, so that the CT-carbon appeared not to be absorbed from the small intestine. In Expt 3, rumen, abomasal and ileal digesta and faeces samples from sheep fed on Lotus pedunculatus were analysed for CT and CT flow along the digestive tract calculated from reference to indigestible markers. Values were low in all digesta samples, indicating disappearance of CT across the rumen and small intestine, and CT recovery in faeces was only about 15% of intake. However, the 14C results from Expt 2 suggested that little if any CT-carbon was absorbed and the low recoveries in Expt 1 are considered to be a consequence of either conformational changes to the CT molecule such that it is no longer detectable by colorimetric methods, an inability of the analytical method to release bound CT for the butanol–HCI assay, or interference from other digesta constituents. It is concluded that the butanol–HCI method of CT analysis is appropriate for quantifying CT in herbages but not in digesta or faeces, and that a substantial part of CT released during protein digestion in the small intestine may not be detectable by normal CT analytical methods.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep
      Available formats
      ×
Copyright
References
Hide All
Barry T. N. (1989). Condensed tannins; their role in ruminant protein and carbohydrate digestion and possible effects upon the rumen ecosystem. In The Role of Protozoa and Fungi in Ruminant Digestion, pp. 153169 [Nolan J. V., Leng R. A. and Demeyer D. I., editors]. Armidale, Australia: Penambal Books.
Bate-Smith E. C. (1973). Tannins of herbaceous leguminosae. Phytochemistry 12, 18091812.
Broadhurst R. B. & Jones W. T. (1978). Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture 29, 788794.
Foo L. Y., Jones W. T., Porter L. J. & Williams V. M. (1982). Proanthocyanidin polymers of fodder legumes. Phytochemistry 21, 933935.
Hinks N. T., Mills S. C. & Setchell B. P. (1966). A simple method for the determination of specific activity of carbon dioxide in blood. Analytical Biochemistry 17, 551553.
Jones W. T., Broadhurst R. B. & Lyttleton J. W. (1976). The condensed tannins of pasture legume species. Phytochemistryl 15, 14071409.
Jones W. T. & Mangan J. L. (1977). Complexes of the condensed tannins of the sainfoin (Onobrychis viciifolia Scop.) with Fraction 1 leaf protein and with submaxillary muco-protein, and their reversal by polyethylene glycol and pH. Journal of the Science of Food and Agriculture 28, 126136.
Lai R., Woolley D. J. & Lawes G. S. (1988). Patterns of assimilate transport from leaves to fruit within a kiwifruit (Acfinidia deliciosa) lateral. Journal of Horticultural Science 63, 725730.
McNabb W. C., Waghorn G. C., Barry T. N. & Shelton I. D. (1993). The effect of condense tannins in Lotus pedunculatus on the digestion and metabolism of methionine, cystine and inorganic sulphur in sheep. British Journal of Nutrition 70, 647661.
Mangan J. L. (1988). Nutritional effects of tannins in animal feeds. Nutrition Research Reviews 1, 209231.
Porter L. J., Hrstich L. N. & Chan B. C. (1986). The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 25, 223230.
Sarkar S. K., Howarth R. E. & Golpen B. P. (1976). Condensed tannins in herbaceous legumes. Crop Science 16, 543546.
Terrill T. H., Douglas G. B., Foote A. G., Purchas R. W., Wilson G. F. & Barry T. N. (1992 a). Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum coronarium) and perennial pasture. Journal of Agricultural Science, Cambridge 119, 265273.
Terrill T. H., Rowan A. M., Douglas G. B. & Barry T. N. (1992 b). Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture 58, 321329.
Terrill T. H., Windham W. R., Evans J. J. & Hoveland C. S. (1990). Condensed tannin concentrations in Sericea lespedeza, as influenced by preservation method. Crop Science 30, 219224.
Terrill T. H., Windham W. R., Hoveland C. S. & Amos H. E. (1989). Forage preservation method influences on tannin concentration, intake and digestibility of Sericea Iespedeza by sheep. Agronomy Journal 81, 435439.
Waghorn G.C., Shelton I. D. & McNabb W. C. (1994 a). Effects of condensed tannins in Lotuspedunculctus on its nutritive value for sheep. 1. Non-nitrogenous aspects. Journal of Agricultural Science, Cambridge 123, 99107.
Waghorn G.C., Shelton I. D., McNabb W. C. & McCutcheon S. N. (1994 b). Effects of condensed tannin in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. Journal of Agricultural Science, Cambridge 123, 109119.
Yn F., Barry T. N., Moughan P. J. & Wilson G.F. (1993). The concentration of condensed tannin and gossypol in cottonseed and in processed cottonseed meal. Journal of the Science of Food and Agriculture 63, 715.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 261 *
Loading metrics...

Abstract views

Total abstract views: 561 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 24th November 2017. This data will be updated every 24 hours.