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Boerhaaviadiffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-κβ and transforming growth factor β1

  • A. Prathapan (a1), V. P. Vineetha (a1), P. A. Abhilash (a2) and K. G. Raghu (a1)
Abstract

The present study evaluated the antihypertrophic potential of the ethanolic extract of Boerhaavia diffusa (BDE), a well-known edible cardiotonic plant reported in Ayurveda against angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells. Markers of hypertrophy such as cell size, protein content and the concentrations of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) were analysed for the confirmation of hypertrophy induction. Angiotensin II (100 nm) caused an increase in cell volume (69·26 (sd 1·21) %), protein content (48·48 (sd 1·64) %), ANP (81·90 (sd 1·22) %) and BNP (108·57 (sd 1·47) %). BDE treatment significantly reduced cell volume, protein content and the concentrations of ANP and BNP (P≤ 0·05) in H9c2 cells. The activity of various antioxidant enzymes and the concentration of reduced glutathione, which was lowered due to hypertrophy, were increased in BDE-treated cells. The BDE treatment also reduced intracellular reactive oxygen species generation, lipid peroxidation and protein carbonyls in cells. In addition, the expression patterns of NF-κβ and transforming growth factor β1 were found to be increased during hypertrophy, and their expressions were reduced on BDE treatment. In vitro chemical assays showed that BDE inhibits angiotensin-converting enzyme and xanthine oxidase in a dose-dependent manner with an estimated 50 % effective concentration (EC50) value of 166·12 (sd 2·42) and 60·05 (sd 1·54) μg/ml, respectively. The overall results clearly indicate the therapeutic potential of B. diffusa against cardiac hypertrophy, in addition to its nutritional qualities.

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      Boerhaaviadiffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-κβ and transforming growth factor β1
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Corresponding author
*Corresponding author: Dr K. G. Raghu, fax +91 471 2491712/2491585, email raghukgopal2009@gmail.com
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1 MH Drazner , JE Rame , EK Marino , et al. (2004) Increased left ventricular mass is a risk factor for the development of a depressed left ventricular ejection fraction within five years: the Cardiovascular Health Study. J Am Coll Cardiol 43, 22072215.

2 CJ Liu , YC Cheng , KW Lee , et al. (2008) Lipopolysaccharide induces cellular hypertrophy through calcineurin/NFAT-3 signalling pathway in H9c2 myocardiac cells. Mol Cell Biochem 313, 167178.

4 FR Yao , CW Sun & SK Chang (2010) Morton lentil extract attenuated angiotensin II induced cardiomyocyte hypertrophy via inhibition of intracellular reactive oxygen species levels in vitro. J Agric Food Chem 58, 1038210388.

5 E Takimoto & DA Kass (2007) Role of oxidative stress in cardiac hypertrophy and remodelling. Hypertension 49, 241248.

6 HL Li , AB Wang , Y Huang , et al. (2005) Isorhapontigenin, a new resveratrol analog, attenuates cardiac hypertrophy via blocking signalling transduction pathways. Free Rad Biol Med 38, 243257.

7 EM Alissa & GA Ferns (2012) Functional foods and nutraceuticals in the primary prevention of cardiovascular diseases. J Nutr Metabol 2012, article ID 569486.

8 MK Ames , MK Shigenaga & TM Hagen (1993) Oxidants, antioxidants and the degenerative disease of aging. Proc Natl Acad Sci U S A 90, 79157922.

9 MJ Stampfer , FB Hu , JE Manson , et al. (2000) Primary prevention of coronary heart disease in women through diet and lifestyle. New Eng J Med 343, 1622.

11 R Srivastava , D Saluja , BS Dwarakanath , et al. (2011) Inhibition of human cervical cancer cell growth by ethanolic extract of Boerhaavia diffusa Linn. (Punarnava) root. Evid Based Complement Alternat Med 2011, article ID 427031.

12 AS Apu , MS Liza , ATM Jamaluddin , et al. (2012) Phytochemical screening and in vitro bioactivities of the extracts of aerial part of Boerhaavia diffusa Linn. Asian Paci J Trop Biomed 2, 673678.

13 TCJ Munasinghe , CK Seneviratne , IM Thabrew , et al. (2001) Antiradical and antilipoperoxidative effects of some plant extracts used by Sri Lankan traditional medical practitioners for cardioprotection. Phytother Res 15, 519523.

15 L Pari & AM Satheesh (2004) Antidiabetic effect of Boerhaavia diffusa: effect on serum and tissue lipids in experimental diabetes. J Med Food 7, 472476.

16 KA Manu & G Kuttan (2009) Immunomodulatory activities of Punarnavine, an alkaloid from Boerhaavia diffusa. Immunopharmacol Immunotoxicol 31, 377387.

17 S Sreeja & S Sreeja (2009) An in vitro study on antiproliferative and antiestrogenic effects of Boerhaavia diffusa L. extracts. J Ethnopharmacol 126, 221225.

18 G Aviello , JM Canadanovic-Brunet , N Milic , et al. (2011) Potent antioxidant and genoprotective effects of Boeravinone G, a rotenoid isolated from Boerhaavia diffusa. Plos One 6, e19628.

19 A Prathapan , MK Singh , SS Anusree , et al. (2011) Antiperoxidative, free radical scavenging and metal chelating activities of Boerhaavia diffusa L. J Food Biochem 35, 15481554.

20 V Gulati , IH Harding & EA Palombo (2012) Enzyme inhibitory and antioxidant activities of traditional medicinal plants: potential application in the management of hyperglycemia. BMC Compl Alt Med 12, 77.

21 SK Pareta , KC Patra , PM Mazumder , et al. (2011) Aqueous extract of Boerhaavia diffusa root ameliorates ethylene glycol-induced hyperoxaluric oxidative stress and renal injury in rat kidney. Pharm Biol 49, 12241233.

24 B Hernandez-Ledesma , PJ Martin-Alvarez & E Pueyo (2003) Assessment of the spectrometric method for determination of angiotensin-converting enzyme activity: influence of the inhibition type. J Agri Food Chem 51, 41754179.

25 PL Owen & T Johns (1999) Xanthine oxidase inhibitory activity of northeastern North American plant remedies used for gout. J Ethnopharmacol 64, 149160.

27 KE Merten , Y Jiang , W Feng , et al. (2006) Calcineurin activation is not necessary for Doxorubicin-induced hypertrophy in H9c2 embryonic rat cardiac cells: involvement of the phosphoinositide 3-kinase-Akt pathway. J Pharmacol Exp Therap 319, 934940.

28 MM Bradford (1976) A rapid and sensitive method for the quantification of microgram quantities of protein, utilizing the principle of protein-dye binding. Anal Biochem 72, 248.

29 H Aebi (1984) Catalase in vitro. Methods Enzymol 105, 121127.

30 F Paoletti , D Aldinucci , A Mocali , et al. (1986) A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal Biochem 154, 538541.

33 PJ Hissin & RA Hilf (1976) Fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74, 214226.

34 EH Choi , HJ Chang , JY Cho , et al. (2007) Cytoprotective effect of anthocyanins against doxorubicin-induced toxicity in H9c2 cardiomyocytes in relation to their antioxidant activities. Food Chem Toxicol 45, 18731881.

35 KG Raghu & OL Cherian (2009) Characterization of cytotoxicity induced by arsenic trioxide (a potent anti-APL drug) in rat cardiac myocytes. J Trace Elem Med Biol 23, 6168.

36 P Chomczynski & N Sacchi (1987) Singlet-step method of RNA isolation by acid guanidium thiocyanate–phenol–chloroform extraction. Anal Biochem 162, 156159.

37 BW Kimes & BL Brandt (1976) Properties of a clonal muscle cell line from rat heart. Exp Cell Res 98, 367381.

38 SJ Watkins , GM Borthwick & HM Arthur (2011) The H9c2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro. In vitro Cell Dev Biol Animal 47, 125131.

39 J Hescheler , R Meyer , S Plant , et al. (1991) Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9c2) line from rat heart. Circ Res 69, 14761486.

40 KR Sipido & E Marban (1991) L-type calcium channels, potassium channels, and novel nonspecific cation channels in a clonal muscle cell line derived from embryonic rat ventricle. Circ Res 69, 14871499.

41 KC Wollert & H Drexler (1999) The rennin–angiotensin system and experimental heart failure. Cardiovasc Res 43, 838849.

42 T Unger (2002) The role of the renin–angiotensin system in the development of cardiovascular disease. Am J Cardiol 89, 3A9A.

43 H Wang & JA Joseph (1999) Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Rad Biol Med 27, 612616.

44 RJ Hajjar & JA Leopold (2006) Xanthine oxidase inhibition and heart failure. Circ Res 98, 169171.

45 B Poljsak (2011) Strategies for reducing or preventing the generation of oxidative stress. Oxid Med Cell Longev 2011, 194586.

46 D Dinu , GO Bodea , CD Ceapa , et al. (2011) Adapted response of the antioxidant defense system to oxidative stress induced by deoxynivalenol in Hek-293 cells. Toxicon 57, 10271032.

48 SK Nelson , SK Bose & JM McCord (1994) The toxicity of high-dose superoxide dismutase suggests that superoxide can both initiate and terminate lipid peroxidation in the reperfused heart. Free Radic Biol Med 16, 195200.

49 M Shlafer & BM Shepard (1984) A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances. Anal Biochem 137, 269276.

50 B Halliwell (1996) Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 25, 5774.

51 X Peng & Y Li (2002) Induction of cellular glutathione linked enzymes and catalase by the unique chemo preventive agent, 3H-1,2-dithiole-3-thione in rat cardiomyocytes affords protection against oxidative cell injury. Pharmacol Res 45, 491497.

52 PA Abhilash , R Harikrishnan & M Indira (2012) Ascorbic acid supplementation causes faster restoration of reduced glutathione content in the regression of alcohol induced hepatotoxicity in male guinea pigs. Redox Rep 17, 7279.

53 KD Kent , WJ Harper & JA Bomser (2003) Effect of whey protein isolate on intracellular glutathione and oxidant-induced cell death in human prostate epithelial cells. Toxicol in vitro 17, 2733.

54 X Ouyang , K Takahashi , K Komatsu , et al. (2001) Protective effect of Salvia militiorrhiza on angiotensin II induced hypertrophic responses in neonatal rat cardiac cells. Jpn J Pharmacol 87, 289296.

55 H Ichikawa , H Yagi , T Tanaka , et al. (2010) Lagerstroemia speciosa extract inhibit TNF-induced activation of nuclear factor-κβ in rat cardiomyocyte H9c2 cells. J Ethnopharmacol 128, 254256.

56 NH Purcell , G Tang , C Yu , et al. (2001) Activation of NF-κβ is required for hypertrophic growth of primary rat neonatal ventricular cardiomyocytes. Proc Natl Acad Sci U S A 98, 66686673.

57 Y Li , T Ha , X Gao , et al. (2004) NF-κB activation is required for the development of cardiac hypertrophy in vivo. Am J Physiol Heart Circ Physiol 287, 17121720.

58 AS Baldwin Jr (1996) The NF-kappa B and I-kappa B proteins: new discoveries and insights. Ann Rev Immunol 14, 649683.

59 WK Jones , M Brown , M Wilhide , et al. (2005) NF κβ in cardiovascular disease. Cardiovasc Toxicol 5, 183201.

60 RK Li , G Li , DA Mickle , et al. (1997) Overexpression of transforming growth factor-beta1 and insulin-like growth factor-I in patients with idiopathic hypertrophic cardiomyopathy. Circulation 96, 874881.

61 JJ Schultz , SA Witt , BJ Glascock , et al. (2002) TGF-β1 mediates the hypertrophic cardiomyocyte growth induced by angiotensin II. J Clin Invest 109, 787796.

62 T Yamazaki , N Yamashita , Y Izumi , et al. (2012) The antifibrotic agent pirfenidone inhibits angiotensin II-induced cardiac hypertrophy in mice. Hypertension Res 35, 3440.

64 F Ferreres , C Sousa , M Justin , et al. (2005) Characterization of phenolic profile of Boerhaavia diffusa L. by HPLC-PAD-MS/MS as tool for quality control. Phytochem Anal 16, 451458.

66 F Perez-Vizcaino & J Duarte (2010) Flavonols and cardiovascular disease. Mol Aspects Med 31, 478494.

67 J Duarte , R Jimenez , F O'Valle , et al. (2002) Protective effects of the flavonoid quercetin in chronic nitric oxide deficient rats. J Hypertens 20, 18431854.

68 JM Calderon-Montano , E Burgos-Moron , C Perez-Guerrero , et al. (2011) A review on the dietary flavonoid kaempferol. Mini Rev Med Chem 11, 298344.

69 S Senthil , M Sridevi & KV Pugalendi (2007) Protective effect of ursolic acid against myocardial ischemia induced by isoproterenol in rats. Toxicol Mech Methods 17, 5765.

70 N Lami , S Kadota , T Kikuchi , et al. (1991) Constituents of the roots of Boerhaavia diffusa L. III. Identification of a calcium channel antagonistic compound from the methanol extract. Chem Pharm Bull 39, 15511555.

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