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Carotenoids are more bioavailable from papaya than from tomato and carrot in humans: a randomised cross-over study

  • Ralf M. Schweiggert (a1) (a2), Rachel E. Kopec (a2) (a3), Maria G. Villalobos-Gutierrez (a4), Josef Högel (a5), Silvia Quesada (a6), Patricia Esquivel (a4), Steven J. Schwartz (a2) (a3) and Reinhold Carle (a1)...

Carrot, tomato and papaya represent important dietary sources of β-carotene and lycopene. The main objective of the present study was to compare the bioavailability of carotenoids from these food sources in healthy human subjects. A total of sixteen participants were recruited for a randomised cross-over study. Test meals containing raw carrots, tomatoes and papayas were adjusted to deliver an equal amount of β-carotene and lycopene. For the evaluation of bioavailability, TAG-rich lipoprotein (TRL) fractions containing newly absorbed carotenoids were analysed over 9·5 h after test meal consumption. The bioavailability of β-carotene from papayas was approximately three times higher than that from carrots and tomatoes, whereas differences in the bioavailability of β-carotene from carrots and tomatoes were insignificant. Retinyl esters appeared in the TRL fractions at a significantly higher concentration after the consumption of the papaya test meal. Similarly, lycopene was approximately 2·6 times more bioavailable from papayas than from tomatoes. Furthermore, the bioavailability of β-cryptoxanthin from papayas was shown to be 2·9 and 2·3 times higher than that of the other papaya carotenoids β-carotene and lycopene, respectively. The morphology of chromoplasts and the physical deposition form of carotenoids were hypothesised to play a major role in the differences observed in the bioavailability of carotenoids from the foods investigated. Particularly, the liquid-crystalline deposition of β-carotene and the storage of lycopene in very small crystalloids in papayas were found to be associated with their high bioavailability. In conclusion, papaya was shown to provide highly bioavailable β-carotene, β-cryptoxanthin and lycopene and may represent a readily available dietary source of provitamin A for reducing the incidence of vitamin A deficiencies in many subtropical and tropical developing countries.

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      Carotenoids are more bioavailable from papaya than from tomato and carrot in humans: a randomised cross-over study
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* Corresponding author: R. M. Schweiggert, email
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1 WHO (2009) Global prevalence of vitamin A deficiency in populations at risk 1995–2005. WHO Global Database on Vitamin A Deficiency 119.
2 Böhm, V (2012) Lycopene and heart health. Mol Nutr Food Res 56, 296303.
3 Harrison, EH (2012) Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids. Biochim Biophys Acta 1821, 7077.
4 Borel, P (2003) Factors affecting intestinal absorption of highly lipophilic food microconstituents (fat-soluble vitamins, carotenoids and phytosterols). Clin Chem Lab Med 41, 979994.
5 Castenmiller, JJM & West, CE (1998) Bioavailability and bioconversion of carotenoids. Annu Rev Nutr 18, 1938.
6 Van Het Hof, KH, Brouwer, IA, West, CE, et al. (1999) Bioavailability of lutein from vegetables is 5 times higher than that of b-carotene. Am J Clin Nutr 70, 261268.
7 Kostic, D, White, WS & Olson, JA (1995) Intestinal absorption, serum clearance, and interactions between lutein and β-carotene when administered to human adults in separate or combined oral doses. Am J Clin Nutr 62, 604610.
8 van het Hof, KH, West, CE, Weststrate, JA, et al. (2000) Dietary factors that affect the bioavailability of carotenoids. J Nutr 130, 503506.
9 Tyssandier, V, Cardinault, N, Caris-Veyrat, C, et al. (2002) Vegetable-borne lutein, lycopene, and β-carotene compete for incorporation into chylomicrons, with no adverse effect on the medium-term (3-wk) plasma status of carotenoids in humans. Am J Clin Nutr 75, 526534.
10 Brown, MJ, Ferruzzi, MG, Nguyen, ML, et al. (2004) Carotenoid bioavailability is higher from salads ingested with full-fat than with fat-reduced salad dressings as measured with electrochemical detection. Am J Clin Nutr 80, 396403.
11 Riedl, J, Linseisen, J, Hoffmann, J, et al. (1999) Some dietary fibers reduce the absorption of carotenoids in women. J Nutr 129, 21702176.
12 Rock, CL & Swendseid, ME (1992) Plasma β-carotene response in humans after meals supplemented with dietary pectin. Am J Clin Nutr 55, 9699.
13 Schweiggert, RM, Steingass, CB, Heller, A, et al. (2011) Characterization of chromoplasts and carotenoids of red and yellow fleshed papaya (Carica papaya L.). Planta 234, 10311044.
14 Britton, G (2008) Functions of intact carotenoids. In Carotenoids Volume 4: Natural Functions, pp. 189236 [Britton, G, Liaaen-Jensen, S and Pfander, H, editors]. Basel: Birkhäuser Verlag.
15 Rock, CL, Lovalvo, JL, Emenhiser, C, et al. (1998) Bioavailability of β-carotene is lower in raw than in processed carrots and spinach in women. J Nutr 128, 913916.
16 Vásquez-Caicedo, AL, Heller, A, Neidhart, S, et al. (2006) Chromoplast morphology and β-carotene accumulation during postharvest ripening of mango cv. ‘Tommy Atkins’. J Agric Food Chem 54, 57695776.
17 Schweiggert, RM, Mezger, D, Schimpf, F, et al. (2012) Influence of chromoplast morphology on carotenoid bioaccessibility of carrot, mango, papaya, and tomato. Food Chem 60, 25772585.
18 Schweiggert, RM, Steingass, CB, Esquivel, P, et al. (2012) Chemical and morphological characterization of Costa Rican papaya (Carica papaya L.) hybrids and lines with particular focus on their genuine carotenoid profiles. J Agric Food Chem 60, 25772585.
19 US Department of Agriculture (2011) Nutrient data laboratory home page. USDA National Nutrient Database for Standard Reference Release 24. (accessed July 2011).
20 Terpstra, AHM (1985) Isolation of serum chylomicrons prior to density gradient ultracentrifugation of other serum lipoprotein classes. Anal Biochem 150, 221227.
21 O'Neill, ME & Thurnham, DI (1998) Intestinal absorption of β-carotene, lycopene and lutein in men and women following a standard meal: response curves in the triacylglycerol-rich lipoprotein fraction. Br J Nutr 79, 149159.
22 Schweiggert, RM, Steingass, CB, Mora, E, et al. (2011) Carotenogenesis and physico-chemical characteristics during maturation of red fleshed papaya fruit (Carica papaya L.). Food Res Int 44, 13731380.
23 Kopec, RE, Schweiggert, RM, Riedl, K, et al. (2012) Comparison of HPLC–MS/MS and HPLC-DAD for the quantitation of carotenoids, retinyl esters, α-tocopherol, and phylloquinone in chylomicron-rich fractions of human plasma. Rapid Commun Mass Spectrom 27, 13931402.
24 Britton, G (1995) UV/visible spectroscopy. In Carotenoids. Volume 1B: Spectroscopy, pp. 1362 [Britton, G, Liaaen-Jensen, S and Pfander, H, editors]. Basel/Boston/Berlin: Birkhäuser Verlag.
25 Unlu, NZ, Bohn, T, Clinton, SK, et al. (2005) Carotenoid absorption from salad and salsa by humans is enhanced by the addition of avocado or avocado oil. J Nutr 135, 431436.
26 Lemmens, L, Van Buggenhout, S, Van Loey, AM, et al. (2010) Particle size reduction leading to cell wall rupture is more important for the β-carotene bioaccessibility of raw compared to thermally processed carrots. J Agric Food Chem 58, 1276912776.
27 Read, NW, Cammack, J & Edwards, C (1982) Is the transit time of a meal through the small intestine related to the rate at which it leaves the stomach? Gut 23, 824828.
28 Faulks, RM, Hart, DJ, Brett, GM, et al. (2004) Kinetics of gastro-intestinal transit and carotenoid absorption and disposal in ileostomy volunteers fed spinach meals. Eur J Nutr 43, 1522.
29 Tang, R, Orme, CA & Nancollas, GH (2004) Dissolution of crystallites: surface energetic control and size effects. ChemPhysChem 5, 688696.
30 Liversidge, GG & Cundy, KC (1995) Particle size reduction for improvement of oral bioavailability of hydrophobic drugs: I. Absolute oral bioavailability of nanocrystalline danazol in beagle dogs. Int J Pharm 125, 9197.
31 Frey-Wyssling, A & Kreutzer, E (1958) The submicroscopic development of chromoplasts in the fruit of Capsicum annuum L. J Ultrastruct Res 1, 397411.
32 Sitte, P, Falk, H & Liedvogel, B (1980) Chromoplasts. In Pigments in Plants, pp. 117148 [Czygan, F, editor]. Stuttgart: G Fischer.
33 Institute of Medicine (2001) Vitamin A. Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc, pp. 82161, [Institute of Medicine, Food and Nutrition Board, editor]. Washington, DC: National Academic Press.
34 Burri, BJ, Chang, JST & Neidlinger, TR (2011) β-Cryptoxanthin- and α-carotene-rich foods have greater apparent bioavailability than β-carotene-rich foods in Western diets. Br J Nutr 105, 212219.
35 Borel, P, Grolier, P, Armand, M, et al. (1996) Carotenoids in biological emulsions: solubility, surface-to-core distribution, and release from lipid droplets. J Lipid Res 37, 250261.
36 Dhuique-Mayer, C, Borel, P, Reboul, E, et al. (2007) β-Cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model. Br J Nutr 97, 883890.
37 Davis, C, Jing, H, Howe, JA, et al. (2008) β-Cryptoxanthin from supplements or carotenoid-enhanced maize maintains liver vitamin A in Mongolian gerbils (Meriones unguiculatus) better than or equal to β-carotene supplements. Br J Nutr 100, 786793.
38 De Roos, AJ, Arab, L, Renner, JB, et al. (2001) Serum carotenoids and radiographic knee osteoarthritis: the Johnston County Osteoarthritis Project. Public Health Nutr 4, 935942.
39 Cerhan, JR, Saag, KG, Merlino, LA, et al. (2003) Antioxidant micronutrients and risk of rheumatoid arthritis in a cohort of older women. Am J Epidemiol 157, 345354.
40 Maggio, D, Polidori, MC, Barabani, M, et al. (2006) Low levels of carotenoids and retinol in involutional osteoporosis. Bone 38, 244248.
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British Journal of Nutrition
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