Skip to main content Accessibility help
×
Home

Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation

  • Wataru Sumiyoshi (a1) (a2), Tadasu Urashima (a1), Tadashi Nakamura (a1), Ikichi Arai (a1), Tadao Saito (a3), Norihiko Tsumura (a4), Bing Wang (a5), Janette Brand-Miller (a5), Yoko Watanabe (a6) and Kazumasa Kimura (a6)...

Abstract

Using reverse-phase HPLC after pyridylamination, we quantified the concentrations of major neutral oligosaccharides in the milk of sixteen Japanese women collected at 4, 10, 30 and 100 d postpartum. In colostrum and mature milk (30 d lactation), lacto-N-fucopentaose (LNFP) I was the most abundant oligosaccharide, followed by 2′-fucosyllactose (2′-FL) + lacto-N-difucotetraose (LNDFT), LNFP II + lacto-N-difucohexaose II (LNDFH II), and 3-fucosyllactose (3-FL). Together these accounted for 73 % of the total weight of neutral oligosaccharides in colostrum and mature milk. Changes in concentration occurred during the course of lactation. LNFP I and 2′-FL + LNDFT increased from 4 to 10 d postpartum, and then declined by 100 d. LNFP II + LNDFH II steadily increased during the first 30 d and then declined. In contrast, 3-FL increased steadily throughout the entire 100 d of study. Large differences were observed between our data and previously published data in Italian women, in terms of both the concentration and temporal changes of each oligosaccharide. These differences may be caused by different assay methodology, although racial differences cannot be ruled out.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation
      Available formats
      ×

Copyright

Corresponding author

*Corresponding Author: Dr T. Urashima, fax +81 155 49 5577, email urashima@obihiro.ac.jp

References

Hide All
Andersson, B, Porras, O, Hanson, , Lagergård, T & Svanborg-Edén, C (1986) Inhibition of attachment of Streptococcus pneumoniae and Haemophilus influenzae by human milk and receptor oligosaccharides. Journal of Infectious Disease 153, 232237.
Cervantes, LE, Newburg, DS & Ruiz-Palacios, GM (1995) α 1-2 fucosylated chains (H-2 and Lewis b) are the main human milk receptor analogs for Campylobacter. Pediatric Research 37, 171A.
Chaturvedi, P, Warren, CD, Altaye, M, Morrow, AL, Ruiz-Palacios, G, Pickering, LK & Newburg, DS (2001) Fucosylated human milk oligosaccharides vary between individuals and over the course of lactation. Glycobiology 11, 365372.
Chaturvedi, P, Warren, CD, Ruiz-Palacios, GM, Pickering, LK & Newburg, DS (1997) Milk oligosaccharide profiles by reversed-phase HPLC of their perbenzoylated derivatives. Analytical Biochemistry 251, 8997.
Coppa, GV, Bruni, S, Zampini, L, Galeazzi, T, Capretti, R, Facinelli, B & Gabrielli, O (2000) Human milk oligosaccharides inhibit the adhesion of Listeria monocytogenes, Escherichia coli Vibrio cholerae and Salmonella fyris. In In 20th International Carbohydrate Symposium, Hamburg, 27 August–1 September 2000. Abstract, International Carbohydrate Organization.
Coppa, GV, Gabrielli, O, Giorge, P, Catassi, C, Montanari, MP, Varaldo, PE & Nichols, BL (1990) Preliminary study of breastfeeding and bacterial adhesion to uroepithelial cells. Lancet 335, 569571.
Coppa, GV, Pierani, P, Zampini, L, Carloni, I & Gabrielli, O (1999) Oligosaccharides in human milk during different phases of lactation. Acta Paediatrica 430, Suppl., 8994.
Coppa, GV, Pierani, P, Zampini, L, Gabrielli, O, Carlucci, A, Catassi, C & Giorgi, PL (1997) Lactose, oligosaccharide and monosaccharide content of milk from mothers delivering preterm newborns over the first month of lactation. Minerva Pediatrica 49, 471475.
Cravioto, A, Tello, A, Villafán, H, Ruiz, J, Vedovo, S & Nesser, JR (1991) Inhibition of localized adhesion of enteropathogenic Escheichia coli to Hep-2 cells by immunoglobulin and oligosaccharide fractions of human colostrums and breast milk. Journal of Infectious Disease 163, 12471255.
Dai, D, Nanthkumar, NN, Newburg, DS & Walker, WA (1990) Role of oligosaccharides and glycoconjugates in intestinal host defense. Journal of Pediatric Gastroenterology and Nutrition 30, s23s33.
Dubois, M, Gilles, KA, Hamilton, JK, Rebers, PA & Smith, F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350356.
Erney, RM, Malone, WT, Skelding, MB, Marcon, AA, Kleman-Leyer, KM, O'Ryan, ML, Ruiz-Palacios, G, Hilty, MD, Pickering, LK & Prieto, PA (2000) Variability of human milk neutral oligosaccharides in a diverse population. Journal of Pediatric Gastroenterology and Nutrition 30, 181192.
Farkas, E, Thiem, J & Ajisaka, K (2000) Enzymatic synthesis of fucose-containing disaccharides employing the partially purified α-L-fucosidase from Penicillium multicolor. Carbohydrate Research 328, 293299.
Gÿorgy, P, Norris, RF & Rose, CS (1954) Bifidus factor. I. A variant of Lactobacillus bifidus requiring a special growth factor. Archives of Biochemistry and Biophysics 48, 193201.
Gÿorgy, P & Rose, CS (1955) Microbiological Studies on Growth Factor for L. bifidus var. pennsylvanicus. Proceedings of the Society for Experimental Biology and Medicine 90, 219223.
Hase, S (1994) High-performance liquid chromatography of pyridylaminated saccharides. Methods in Enzymology 230, 225237.
Idota, T, Kawakami, H, Murakami, Y & Sugawara, M (1995) Inhibition of cholera toxin by human milk fractions and sialyllactose. Bioscience, Biotechnology, and Biochemistry 59, 417419.
Jourdian, GW, Dean, L & Roseman, S (1971) The sialic acids XI. A periodate-resorcinol method for quantitative estimation of free sialic acids and their glycosides. Journal of Biological Chemistry 246, 430435.
Kimura, K, Matsumoto, K, Ishihara, C, Harada, K & Miyagi, A (1995) Structure determination of galacto-oligosaccharides by pyridylamintion and NMR spectroscopy. Carbohydrate Research 270, 3342.
Kimura, K, Watanabe, Y, Matsumoto, K & Miyagi, A (1997) Studies on the natural galacto-oligosaccharides in commercial cow's milk. YAKULT Institute Report 17, 17.
Kondo, A, Suzuki, J, Kuraya, N, Hase, S, Kato, I & Ikenaka, T (1990) Improved method for fluorescence labeling of sugar chains with sialic acid residues. Agricultural and Biological Chemistry 54, 21692170.
Kunz, C & Rudloff, S (1993) Biological functions of oligosaccharides in human milk. Acta Paediatrica 82, 903912.
McVeagh, P & Miller, JB (1997) Human milk oligosaccharides: only the breast. Journal of Paediatrics and Child Health 33, 281286.
Miller, JB & McVeagh, P (1999) Human milk oligosaccharides: 130 reasons to breast-feed. British Journal of Nutrition 82, 333335.
Murata, T, Inukai, T, Suzuki, M, Yamagishi, M & Usui, T (1999a) Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto-N-neotetraose. Glycoconjugate Journal 16, 189195.
Murata, T, Morimoto, S, Zeng, X, Watanabe, S & Usui, T (1999b) Enzymatic synthesis of α-L-fucosyl-N-acetyllactosamines and 3′-O-α-L-fucosyllactose utilizing α-L-fucosidases. Carbohydrate Research 320, 192199.
Nakhla, T, Fu, D, Zoph, D, Brodsky, NL & Hurt, H (1999) Neutral oligosaccharide content of preterm human milk. British Journal of Nutrition 82, 361367.
Neeser, JR, Golliard, M & Vedovo, SD (1991) Quantitative determination of complex carbohydrates in bovine milk and in milk-based infant formulas. Journal of Dairy Science 74, 28602871.
Neville, MC (1995) Volume of Caloric Density of Human Milk. In Handbook of Milk Composition, pp. 99113 [Jensen, RG, editor]. San Diego, CA: Academic Press.
Newburg, DS (1996) Oligosaccharides and glycoconjugates in human milk: Their role in host defense. Journal of Mammary Gland Biology and Neoplasia 1, 271283.
Newburg, DS (1997) Do the binding properties of oligosaccharides in milk protect human infants from gastrointestinal bacteria? Journal of Nutrition 127, Suppl. 5, 980s984s.
Newburg, DS (1999) Human milk glycoconjugates that inhibit pathogens. Current Medicinal Chemistry 6, 117127.
Newburg, DS (2000) Oligosaccharides in human milk and bacterial colonization. Journal of Pediatric Gastroenterology and Nutrition 30, s8s17.
Newburg, DS & Neubauer, SH (1995) Carbohydrates in milks: Analysis, Quantities, and Significance. In Handbook of Milk Composition, pp. 273349 [Jensen, RG, editor]. San Diego, CA: Academic Press.
Newburg, DS, Pickering, LK, McCluer, RH & Cleary, TG (1999) Fucosylated oligosaccharides of human milk protect suckling mice from heat-stable enterotoxin of Eschericiha coli. Journal of Infectious Disease 162, 10751080.
Portelli, J, Gordon, A & May, JT (1998) Effect of human milk sialyllactose on Cytomegalovirus (letter). European Journal of Clinical Microbiology and Infectious Diseases 17, 66.
Saito, T, Tsuji, T, Kitazawa, H, Kawai, Y & Itoh, T (1998) Application of two-dimensional mapping for an Analysis of Galactosyllactoses in yogurt. Bioscience, Biotechnology, and Biochemistry 62, 14451447.
Stahl, B, Thurl, S, Zeng, J, Karas, M, Hillenkamp, F, Steup, M & Sawatzki, G (1994) Oligosaccharides from human milk as revealed by matrix-assisted laser desorptio/onization mass spectrometry. Analytical Biochemistry 223, 218226.
Suzuki, J, Kondo, A, Kato, I, Hase, S & Ikenaka, T (1991) Analysis by high-performance anion-exchange chromatography of component sugar as their fluorescent pyridylamino derivatives. Agricultural and Biological Chemistry 55, 283284.
Takemoto, H, Hase, S & Ikenaka, T (1985) Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography. Analytical Biochemistry 145, 245250.
Thurl, S, Henker, J, Siegel, M, Tovar, K & Sawatzki, G (1997) Detection of four human milk groups with respect to Lewis blood group dependent oligosaccharides. Glycoconjugate Journal 14, 795799.
Watkins, WM (1996) Glycoproteins: Their Composition, Structure and Function, pp. 462512 [Gottschalk, A, editor]. Amsterdam: Elsevier.
Wiederschain, GY & Newburg, DS (1995) Human milk Fucosyltransferase and α-L-fucosidase activities change during the course of lactation. Journal of Nutritional Biochemistry 6, 582587.
Yanagida, K, Natsuka, S & Hase, S (1999) A pyridylamination method aimed at automatic oligosaccharide analysis of N-linked sugar chains. Analytical Biochemistry 274, 229234.
Yasuno, S, Kokubo, K & Kamei, M (1999) New method for determining the sugar composition of glycoproteins, glycolipids, and oligosaccharides by high-performance liquid chromatography. Bioscience, Biotechnology, and Biochemistry 63, 13531359.
Yoshioka, H, Iseki, K & Fujita, K (1983) Development and differences of intestinal flora in the neonatal period in breast-fed and bottle-fed infants. Pediatrics 72, 317321.
Zopf, D & Roth, S (1996) Oligosaccharide anti-infective agents. Lancet 347, 10171021.

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed