Skip to main content
×
Home
    • Aa
    • Aa

Dietary antioxidants and DNA damage in patients on long-term acid-suppression therapy: a randomized controlled study

  • K. L. M. White (a1), D. M. Chalmers (a2), I. G. Martin (a2), S. M. Everett (a2), P. M. Neville (a2), G. Naylor (a2), A. E. Sutcliffe (a1), M. F. Dixon (a2), P. C. Turner (a1) and C. J. Schorah (a1)...
Abstract

Free radicals and reactive species produced in vivo can trigger cell damage and DNA modifications resulting in carcinogenesis. Dietary antioxidants trap these species limiting their damage. The present study evaluated the role of vitamins C and E in the prevention of potentially premalignant modifications to DNA in the human stomach by supplementing patients who, because of hypochlorhydria and possible depletion of gastric antioxidants, could be at increased risk of gastric cancer. Patients undergoing surveillance for Barrett's oesophagus (n 100), on long-term proton pump inhibitors were randomized into two groups: vitamin C (500 mg twice/d) and vitamin E (100 mg twice/d) for 12 weeks (the supplemented group) or placebo. Those attending for subsequent endoscopy had gastric juice, plasma and mucosal measurements of vitamin levels and markers of DNA damage. Seventy-two patients completed the study. Plasma ascorbic acid, total vitamin C and vitamin E were elevated in the supplemented group consistent with compliance. Gastric juice ascorbic acid and total vitamin C levels were raised significantly in the supplemented group (P=0·01) but supplementation had no effect on the mucosal level of this vitamin. However, gastric juice ascorbic acid and total vitamin C were within normal ranges in the unsupplemented group. Mucosal malondialdehyde, chemiluminescence and DNA damage levels in the comet assay were unaffected by vitamin supplementation. In conclusion, supplementation does not affect DNA damage in this group of patients. This is probably because long-term inhibition of the gastric proton pump alone does not affect gastric juice ascorbate and therefore does not increase the theoretical risk of gastric cancer because of antioxidant depletion.

    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dietary antioxidants and DNA damage in patients on long-term acid-suppression therapy: a randomized controlled study
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Dietary antioxidants and DNA damage in patients on long-term acid-suppression therapy: a randomized controlled study
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Dietary antioxidants and DNA damage in patients on long-term acid-suppression therapy: a randomized controlled study
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author:K. L. M. White, fax +44 113 233 6603, email medklmw@leeds.ac.uk
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

D Bunout , A Garrido , M Suazo , R Kauffman , P Venegas , P de la Maza , M Petermann & S Hirsch (2000) Effects of supplementation with folic acid and antioxidant vitamins on homocysteine levels and LDL oxidation in coronary patients. Nutrition 16, 107110.

GR Davies , NJ Simmonds , TRJ Stevens , A Grandison , DR Blake & DS Rampton (1992) Mucosal reactive oxygen metabolite production in duodenal ulcer disease. Gut 33, 14671472.

MF Dixon , H O'Connor , ATR Axon , RFJG King & D Johnston (1986) Reflux gastritis: distinct histopathological entity? Journal of Clinical Pathology 39, 524530.

IM Drake , NP Mapstone , CJ Schorah , KLM White , DM Chalmers , MF Dixon & ATR Axon (1998) Reactive oxygen species activity and lipid peroxidation in Helicobacter pylori associated gastritis: Relation to gastric mucosal ascorbic acid concentrations and the effect of H. pylori eradication. Gut 42, 768771.

SM Everett , KLM White , CJ Schorah , RJ Calvert , C Skinner , D Miller & ATR Axon (2000) In vivo DNA damage in gastric epithelial cells. Mutation Research 468, 7385.

B Halliwell (1994) Free radicals, antioxidants and human disease. Lancet 344, 721724.

M Hayat , SM Everett , KLM White , IM Drake & S O'Mahony (1998) Ascorbic acid and total vitamin C concentrations in plasma, gastric juice, and gastrointestinal mucosa in lymphocytic gastritis. Gastroenterology 114, A146.

J Lunec (1990) Free radicals: their involvement in disease processes. Annals of Clinical Biochemistry 27, 173182.

C Mowat , A Carswell , A Wirz & KEL McColl (1999) Omeprazole and dietary nitrate independently affect levels of vitamin C and nitrite in gastric juice. Gastroenterology 116, 813822.

GM Sobala , CJ Schorah , M Sanderson , MF Dixon , DS Tomkins , P Godwin & ATR Axon (1989) Ascorbic acid in the human stomach. Gastroenterology 97, 357363.

GM Sobala , CJ Schorah , S Shires , DAF Lynch , B Gallacher , MF Dixon & ATR Axon (1993) Effect of eradication of Helicobacter pylori on gastric juice ascorbic acid concentrations. Gut 34, 10381041.

M Stolte , A Meining , E Seifert & T Alexandridis (2000) Treatment with lansoprazole also induces hypertrophy of the parietal cells of the stomach. Pathology, Research and Practice 196, 913.

K Yagi (1976) A simple fluorometric assay for lipoperoxide in blood plasma. Biochemical Medicine 15, 212216.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: