Skip to main content
×
Home
    • Aa
    • Aa
  • Access
  • Cited by 28
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Barszcz, Marcin Taciak, Marcin and Skomiał, Jacek 2016. The effects of inulin, dried Jerusalem artichoke tuber and a multispecies probiotic preparation on microbiota ecology and immune status of the large intestine in young pigs. Archives of Animal Nutrition, Vol. 70, Issue. 4, p. 278.


    Glahn, Raymond P. Tako, Elad Cichy, Karen and Wiesinger, Jason 2016. The cotyledon cell wall and intracellular matrix are factors that limit iron bioavailability of the common bean (Phaseolus vulgaris). Food Funct., Vol. 7, Issue. 7, p. 3193.


    Kozłowska, Izabela Marć-Pieńkowska, Joanna and Bednarczyk, Marek 2016. 2. Beneficial Aspects of Inulin Supplementation as a Fructooligosaccharide Prebiotic in Monogastric Animal Nutrition – A Review. Annals of Animal Science, Vol. 16, Issue. 2,


    Samolińska, W. and Grela, E. R. 2016. Comparative Effects of Inulin with Different Polymerization Degrees on Growth Performance, Blood Trace Minerals, and Erythrocyte Indices in Growing-Finishing Pigs. Biological Trace Element Research,


    Ancuceanu, Robert Dinu, Mihaela Hovaneţ, Marilena Anghel, Adriana Popescu, Carmen and Negreş, Simona 2015. A Survey of Plant Iron Content—A Semi-Systematic Review. Nutrients, Vol. 7, Issue. 12, p. 10320.


    Hartono, Karen Reed, Spenser Ankrah, Naa Ayikarkor Glahn, Raymond P. and Tako, Elad 2015. Alterations in gut microflora populations and brush border functionality following intra-amniotic daidzein administration. RSC Adv., Vol. 5, Issue. 9, p. 6407.


    Huang, Qianqian Wei, Yinan Lv, Yajun Wang, Yuxi and Hu, Tianming 2015. Effect of dietary inulin supplements on growth performance and intestinal immunological parameters of broiler chickens. Livestock Science, Vol. 180, p. 172.


    Paßlack, Nadine Vahjen, Wilfried and Zentek, Jürgen 2015. Dietary inulin affects the intestinal microbiota in sows and their suckling piglets. BMC Veterinary Research, Vol. 11, Issue. 1, p. 51.


    Cozon, G.J.N. 2014. Carence en fer et troubles digestifs. Transfusion Clinique et Biologique, Vol. 21, Issue. 4-5, p. 189.


    Kortman, Guus A.M. Raffatellu, Manuela Swinkels, Dorine W. and Tjalsma, Harold 2014. Nutritional iron turned inside out: intestinal stress from a gut microbial perspective. FEMS Microbiology Reviews, Vol. 38, Issue. 6, p. 1202.


    Shahzad, Zaigham Rouached, Hatem and Rakha, Allah 2014. Combating Mineral Malnutrition through Iron and Zinc Biofortification of Cereals. Comprehensive Reviews in Food Science and Food Safety, Vol. 13, Issue. 3, p. 329.


    Tako, Elad Glahn, Raymond P Knez, Marija and Stangoulis, James CR 2014. The effect of wheat prebiotics on the gut bacterial population and iron status of iron deficient broiler chickens. Nutrition Journal, Vol. 13, Issue. 1,


    Cerezuela, Rebeca Meseguer, José and Esteban, M. Ángeles 2013. Effects of dietary inulin, Bacillus subtilis and microalgae on intestinal gene expression in gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology, Vol. 34, Issue. 3, p. 843.


    Saad, N. Delattre, C. Urdaci, M. Schmitter, J.M. and Bressollier, P. 2013. An overview of the last advances in probiotic and prebiotic field. LWT - Food Science and Technology, Vol. 50, Issue. 1, p. 1.


    Xue, Xiang and Shah, Yatrik 2013. Intestinal Iron Homeostasis and Colon Tumorigenesis. Nutrients, Vol. 5, Issue. 7, p. 2333.


    Freitas, Karine de Cássia Amancio, Olga Maria Silvério and de Morais, Mauro Batista 2012. High-performance inulin and oligofructose prebiotics increase the intestinal absorption of iron in rats with iron deficiency anaemia during the growth phase. British Journal of Nutrition, Vol. 108, Issue. 06, p. 1008.


    Murgia, Irene Arosio, Paolo Tarantino, Delia and Soave, Carlo 2012. Biofortification for combating ‘hidden hunger’ for iron. Trends in Plant Science, Vol. 17, Issue. 1, p. 47.


    Thakur, Mayank Weng, Alexander Fuchs, Hendrik Sharma, Vikas Bhargava, Chandra Shekhar Chauhan, Nagendra S. Dixit, Vinod K. and Bhargava, Shilpi 2012. Rasayana properties of Ayurvedic herbs: Are polysaccharides a major contributor. Carbohydrate Polymers, Vol. 87, Issue. 1, p. 3.


    Urriola, Pedro E. Cervantes-Pahm, Sarah K. and Stein, Hans H. 2012. Sustainable Swine Nutrition.


    Bauer, Eva Metzler-Zebeli, Barbara U. Verstegen, Martin W. A. and Mosenthin, Rainer 2011. Intestinal gene expression in pigs: effects of reduced feed intake during weaning and potential impact of dietary components. Nutrition Research Reviews, Vol. 24, Issue. 02, p. 155.


    ×

Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine

  • E. Tako (a1), R. P. Glahn (a2), R. M. Welch (a2), X. Lei (a3), K. Yasuda (a2) and D. D. Miller (a1)
  • DOI: http://dx.doi.org/10.1017/S0007114507825128
  • Published online: 01 March 2008
Abstract

Inulin, a linear β fructan, is present in a variety of plants including chicory root and wheat. It exhibits prebiotic properties and has been shown to enhance mineral absorption and increase beneficial bacteria in the colon. The aim of the present study was to assess the effect of dietary inulin on the gene expression of selected intestinal Fe transporters and binding proteins. Anaemic piglets at age 5 weeks were allocated to a standard maize–soya diet (control) or the same diet supplemented with inulin at a level of 4 %. After 6 weeks, the animals were killed and caecum contents and sections of the duodenum and colon were removed. Segments of the genes encoding for the pig divalent metal transporter 1 (DMT1) and duodenal cytochrome-b reductase (Dcytb) were isolated and sequenced. Semi-quantitative RT-PCR analyses were performed to evaluate the expression of DMT1, Dcytb, ferroportin, ferritin, transferrin receptor (TfR) and mucin genes. DMT1, Dcytb, ferroportin, ferritin and TfR mRNA levels in duodenal samples were significantly higher in the inulin group (P ≤ 0·05) compared with the control. In colon, DMT1, TfR and ferritin mRNA levels significantly increased in the inulin group. Additionally, the caecal content microflora was examined using 16S rDNA targeted probes from bacterial DNA. The Lactobacillus and Bifidobacterium populations were significantly increased in the inulin group (P ≤ 0·05) compared with the control group. These results indicate that dietary inulin might trigger an up regulation of genes encoding for Fe transporters in the enterocyte. The specific mechanism for this effect remains to be elucidated.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine
      Your Kindle email address
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine
      Available formats
      ×
Copyright
Corresponding author
*Corresponding author: Dr Elad Tako, fax +1 607 254 4868, email et79@cornell.edu
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

4GO Latunde-Dada , J Van der Westhuizen , CD Vulpe , GJ Anderson , RJ Simpson & AT McKie (2002) Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells Mol Dis 29, 356360.

5K Takeuchi , I Bjarnason , AH Laftah , GO Latunde-Dada , RJ Simpson & AT McKie (2005) Expression of iron absorption genes in mouse large intestine. Scand J Gastroenterol 40, 169177.

6J van Loo , P Coussement , L de Leenheer , H Hoebregs & G Smits (1995) On the presence of inulin and oligofructose as natural ingredients in the Western diet. Crit Rev Food Sci Nutr 35, 525552.

7D Bougle , N Vaghefi-Vaezzadeh , N Roland , G Bouvard , P Arhan , F Bureau , D Neuville & JL Maubois (2002) Influence of short-chain fatty acids on iron absorption by proximal colon. Scand J Gastroenterol 37, 10081011.

8D Bosscher , M Van Caillie-Bertrand , R Van Cauwenbergh & H Deelstra (2003) Availabilities of calcium, iron, and zinc from dairy infant formulas is affected by soluble dietary fibers and modified starch fractions. Nutrition 19, 641645.

10KE Scholz-Ahrens & J Schrezenmeir (2002) Inulin, oligofructose and mineral metabolism – experimental data and mechanism. Br J Nutr 87, Suppl. 2, S179S186.

13L Ellegard , H Andersson & I Bosaeus (1997) Inulin and oligofructose do not influence the absorption of cholesterol, or the excretion of cholesterol, Ca, Mg, Zn, Fe, or bile acids but increases energy excretion in ileostomy subjects. Eur J Clin Nutr 51, 15.

14GR Gibson , ER Beatty , X Wang & JH Cummings (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975982.

16SC Yang , JY Chen , HF Shang , TY Cheng , SC Tsou & JR Chen (2005) Effect of synbiotics on intestinal microflora and digestive enzyme activities in rats. World J Gastroenterol 11, 74137417.

17IJ Broekaert & WA Walker (2006) Probiotics as flourishing benefactors for the human body. Gastroenterol Nurs 29, 2634.

18JH Cummings , EW Pomare , WJ Branch , CP Naylor & GT Macfarlane (1987) Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 28, 12211227.

21MJ Hopkins & GT Macfarlane (2003) Nondigestible oligosaccharides enhance bacterial colonization resistance against Clostridium difficile in vitro. Appl Environ Microbiol 69, 19201927.

23XY Zhu , T Zhong , Y Pandya & RD Joerger (2002) 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol 68, 124137.

25JH Apajalahti , H Kettunen , A Kettunen , WE Holben , PH Nurminen , N Rautonen & M Mutanen (2002) Culture-independent microbial community analysis reveals that inulin in the diet primarily affects previously unknown bacteria in the mouse cecum. Appl Environ Microbiol 68, 49864995.

28HJ Harmsen , GR Gibson , P Elfferich , GC Raangs , AC Wildeboer-Veloo , A Argaiz , MB Roberfroid & GW Welling (2000) Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria. FEMS Microbiol Lett 183, 125129.

31P Eppard , D Bauman , J Bitman , D Wood , R Akers & W House (1985) Effect of dose of bovine growth hormone on milk composition: α-lactalbumin, fatty acids, and mineral elements. J Dairy Sci 68, 30473054.

32P Sharp , S Tandy , S Yamaji , J Tennant , M Williams & SK Singh Srai (2002) Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells. FEBS Lett 510, 7176.

33AT McKie , D Barrow , GO Latunde-Dada , (2001) An iron-regulated ferric reductase associated with the absorption of dietary iron. Science 291, 17551759.

35E Amit-Romach , D Sklan & Z Uni (2004) Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poult Sci 83, 10931098.

36S Ludwiczek , I Theurl , E Artner-Dworzak , M Chorney & G Weiss (2004) Duodenal HFE expression and hepcidin levels determine body iron homeostasis: modulation by genetic diversity and dietary iron availability. J Mol Med 82, 373382.

38GJ Anderson (2001) Ironing out disease: inherited disorders of iron homeostasis. IUBMB Life 51, 1117.

39S Tandy , M Williams , A Leggett , M Lopez-Jimenez , M Dedes , B Ramesh , SK Srai & P Sharp (2000) Nramp2 expression is associated with pH-dependent iron uptake across the apical membrane of human intestinal Caco-2 cells. J Biol Chem 275, 10231029.

40H Gunshin , B Mackenzie , UV Berger , Y Gunshin , MF Romero , WF Boron , S Nussberger , JL Gollan & MA Hediger (1997) Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388, 482488.

41MD Fleming & NC Andrews (1998) Mammalian iron transport: an unexpected link between metal homeostasis and host defense. J Lab Clin Med 132, 464468.

42C Coudray , J Bellanger , C Castiglia-Delavaud , C Remesy , M Vermorel & Y Rayssignuier (1997) Effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. Eur J Clin Nutr 51, 375380.

43L Raschka & H Daniel (2005) Mechanisms underlying the effects of inulin-type fructans on calcium absorption in the large intestine of rats. Bone 37, 728735.

45B Sreedhar & KM Nair (2005) Modulation of aconitase, metallothionein, and oxidative stress in zinc-deficient rat intestine during zinc and iron repletion. Free Radic Biol Med 39, 9991008.

46A Nzeusseu , D Dienst , V Haufroid , G Depresseux , JP Devogelaer & DH Manicourt (2006) Inulin and fructo-oligosaccharides differ in their ability to enhance the density of cancellous and cortical bone in the axial and peripheral skeleton of growing rats. Bone 38, 394399.

47M Staun & S Jarnum (1988) Measurement of the 10,000-molecular weight calcium-binding protein in small-intestinal biopsy specimens from patients with malabsorption syndromes. Scand J Gastroenterol 23, 827832.

52JH Cummings & GT Macfarlane (1991) The control and consequences of bacterial fermentation in the human colon. J Appl Bacteriol 70, 443459.

54J Mentschel & R Claus (2003) Increased butyrate formation in the pig colon by feeding raw potato starch leads to a reduction of colonocyte apoptosis and a shift to the stem cell compartment. Metabolism 52, 14001405.

58S Kolida , K Tuohy & GR Gibson (2002) Prebiotic effects of inulin and oligofructose. Br J Nutr 87, Suppl. 2, S193S197.

59Y Bouhnik , A Attar , FA Joly , M Riottot , F Dyard & B Flourie (2004) Lactulose ingestion increases faecal bifidobacterial counts: a randomised double-blind study in healthy humans. Eur J Clin Nutr 58, 462466.

62K Molly , M Vande Woestyne & W Verstraete (1993) Development of a 5-step multi-chamber reactor as a simulation of the human intestinal microbial ecosystem. Appl Microbiol Biotechnol 39, 254258.

63X Wang & GR Gibson (1993) Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. J Appl Bacteriol 75, 373380.

64CA Heid , J Stevens , KJ Livak & PM Williams (1996) Real time quantitative PCR. Genome Res 6, 986994.

65N Boon , J Goris , P De Vos , W Verstraete & EM Top (2000) Bioaugmentation of activated sludge by an indigenous 3-chloroaniline-degrading Comamonas testosteroni strain, I2gfp. Appl Environ Microbiol 66, 29062913.

67K Daly , MA Cuff , F Fung & SP Shirazi-Beechey (2005) The importance of colonic butyrate transport to the regulation of genes associated with colonic tissue homoeostasis. Biochem Soc Trans 33, 733735.

68R Hass , R Busche , L Luciano , E Reale & WV Engelhardt (1997) Lack of butyrate is associated with induction of Bax and subsequent apoptosis in the proximal colon of guinea pig. Gastroenterology 112, 875881.

70M Simovich , LN Hainsworth , PA Fields , JN Umbreit & ME Conrad (2003) Localization of the iron transport proteins mobilferrin and DMT-1 in the duodenum: the surprising role of mucin. Am J Hematol 74, 3245.

72U Candarian , B Furer , C Hofelein , R Meyer , M Jermini & J Luthy (1991) Detection of Escherichia coli and identification of enterotoxigenic strains by primer-directed enzymatic amplification of specific DNA sequences. Int J Food Microbiol 12, 339351.

73M Denis , J Refregier-Petton , MJ Laisney , G Ermel & G Salvat (2001) Campylobacter contamination in french chicken production from farm to consumers Use of PCR assay for detection and identification of Campylobacter jejunic and Camp. coli. J Appl Microbiol 91, 255267.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: