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Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation

  • Mark C. Fogarty (a1), Ciara M. Hughes (a2), George Burke (a3), John C. Brown (a4) and Gareth W. Davison (a4)...
Abstract

Pharmacological antioxidant vitamins have previously been investigated for a prophylactic effect against exercise-induced oxidative stress. However, large doses are often required and may lead to a state of pro-oxidation and oxidative damage. Watercress contains an array of nutritional compounds such as β-carotene and α-tocopherol which may increase protection against exercise-induced oxidative stress. The present randomised controlled investigation was designed to test the hypothesis that acute (consumption 2 h before exercise) and chronic (8 weeks consumption) watercress supplementation can attenuate exercise-induced oxidative stress. A total of ten apparently healthy male subjects (age 23 (sd 4) years, stature 179 (sd 10) cm and body mass 74 (sd 15) kg) were recruited to complete the 8-week chronic watercress intervention period (and then 8 weeks of control, with no ingestion) of the experiment before crossing over in order to compete the single-dose acute phase (with control, no ingestion). Blood samples were taken at baseline (pre-supplementation), at rest (pre-exercise) and following exercise. Each subject completed an incremental exercise test to volitional exhaustion following chronic and acute watercress supplementation or control. The main findings show an exercise-induced increase in DNA damage and lipid peroxidation over both acute and chronic control supplementation phases (P< 0·05 v. supplementation), while acute and chronic watercress attenuated DNA damage and lipid peroxidation and decreased H2O2 accumulation following exhaustive exercise (P< 0·05 v. control). A marked increase in the main lipid-soluble antioxidants (α-tocopherol, γ-tocopherol and xanthophyll) was observed following watercress supplementation (P< 0·05 v. control) in both experimental phases. These findings suggest that short- and long-term watercress ingestion has potential antioxidant effects against exercise-induced DNA damage and lipid peroxidation.

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Corresponding author
*Corresponding author: Dr G. W. Davison, email gw.davison@ulster.ac.uk
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1ML Urso & PM Clarkson (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189, 4154.

2JM Sacheck , PE Milbury , JG Cannon , et al. (2003) Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men. Free Radic Biol Med 34, 15751588.

5RJ Bloomer , KH Fisher-Wellman , KG Hammond , et al. (2009) Dietary supplement increases plasma norepinephrine, lipolysis, and metabolic rate in resistance trained men. J Int Soc Sports Nutr 6, 1019.

6B Halliwell & M Whiteman (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Clin Pharmacol 142, 231255.

7NB Vollaard , JP Shearman & CE Cooper (2005) Exercise-induced oxidative stress: myths, realities and physiological relevance. Sports Med 35, 10451062.

8A Mastaloudis , JD Morrow , DW Hopkins , et al. (2004) Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radic Biol Med 36, 13291341.

10GW Davison , CM Hughes & RA Bell (2005) Exercise and mononuclear cell DNA damage: the effects of antioxidant supplementation. Int J Sport Nutr Exerc Metab 15, 480492.

11CM Kerksick , RB Kreider & DS Willoughby (2010) Intramuscular adaptations to eccentric exercise and antioxidant supplementation. Amino Acids 39, 219232.

13KA Lyall , SM Hurst , JM Cooney , et al. (2009) Short-term blackcurrant extract consumption modulates exercise-induced oxidative stress and lipopolysaccharide-stimulated inflammatory responses. Am J Physiol Regul Integr Comp Physiol 297, 7081.

16LA Boyd , MJ McCann , Y Hashim , et al. (2006) Assessment of the anti-genotoxic, anti-proliferative, and anti-metastatic potential of crude watercress extract in human colon cancer cells. Nutr Cancer 55, 232241.

19NP Singh , MT McCoy , RR Tice , et al. (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175, 184191.

20AR Collins , AA Oscoz , G Brunborg , et al. (2008) The comet assay: topical issues. Mutagenesis 23, 143151.

23DG Altman (1980) Statistics and ethics in medical research: III How large a sample? Br Med J 281, 13361338.

24MC Fogarty , CM Hughes , G Burke , et al. (2011) Exercise-induced lipid peroxidation: implications for deoxyribonucleic acid damage and systemic free radical generation. Environ Mol Mutagen 52, 3542.

25A Hartmann , U Plappert , K Raddatz , et al. (1994) Does physical activity induce DNA damage? Mutagenesis 9, 269272.

26HE Poulsen , S Loft & K Vistisen (1996) Extreme exercise and oxidative DNA modification. J Sports Sci 14, 343346.

27Z Radak , P Apor , J Pucsok , et al. (2003) Marathon running alters the DNA base excision repair in human skeletal muscle. Life Sci 72, 16271633.

28MC Gomez-Cabrera , J Vina & LL Ji (2009) Interplay of oxidants and antioxidants during exercise: implications for muscle health. Phys Sportsmed 37, 116123.

29MG Nikolaidis & AZ Jamurtas (2009) Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys 490, 7784.

32G Guetens , G De Boeck , M Highley , et al. (2002) Oxidative DNA damage: biological significance and methods of analysis. Crit Rev Clin Lab Sci 39, 331457.

33SK Powers & MJ Jackson (2008) Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 88, 12431276.

35RM Bracken , DM Linnane & S Brooks (2009) Plasma catecholamine and nephrine responses to brief intermittent maximal intensity exercise. Amino Acids 36, 209217.

36B Halliwell & J Gutteridge (2007) Free Radicals in Biology and Medicine, 4th ed.London: Oxford University Press.

37GW Davison , RM Morgan , N Hiscock , et al. (2006) Manipulation of systemic oxygen flux by acute exercise and normobaric hypoxia: implications for reactive oxygen species generation. Clin Sci 110, 133141.

38GW Davison , T Ashton , B Davies , et al. (2008) In vitro electron paramagnetic resonance characterisation of free radical: relevance to exercise-induced lipid peroxidation and implications of ascorbate prohylaxis. Free Radic Res 42, 18.

40VI Lushchak (2007) Free radical oxidation of proteins and its relationship with functional state of organisms. Biochemistry Mosc 72, 809827.

42RJ Bloomer , AH Goldfarb , L Wideman , et al. (2005) Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 19, 276285.

44CC Conaway , YM Yang & FL Chung (2002) Isothiocyanates as cancer chemopreventive agents: their biological activities and metabolism in rodents and humans. Curr Drug Metab 3, 233255.

46L Packer , EH Witt & HJ Tritschler (1995) Alpha-lipoic acid as a biological antioxidant. Free Radic Biol Med 19, 227250.

48W Long 3rd, K Wells , V Englert , et al. (2008) Does prior acute exercise affect post exercise substrate oxidation in response to a high carbohydrate meal? Nutr Metab 5, 25.

49J Pincemail , C Deby , G Camus , et al. (1988) Tocopherol mobilisation during intensive exercise. Eur J Appl Physiol 57, 189191.

50GW Davison , L George , SK Jackson , et al. (2002) Exercise, free radicals, and lipid peroxidation in type 1 diabetes mellitus. Free Radic Biol Med 33, 15431551.

51C McClean , M Clegg , A Shafat , et al. (2011) The impact of acute moderate intensity exercise on arterial regional stiffness, lipid peroxidation and antioxidant status in healthy males. Res Sports Med 19, 113.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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