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Green tea extract selectively activates peroxisome proliferator-activated receptor β/δ in cultured cardiomyocytes

  • Francesca Danesi (a1), Mattia Di Nunzio (a1), Elisa Boschetti (a1) and Alessandra Bordoni (a1)
Abstract

Hypoxia/reoxygenation is one of the causes of the increased expression of inducible NO synthase in cardiomyocytes. In a recent study we demonstrated that a single, high dose of green tea extract (GT) supplemented to the medium of cultured cardiomyocytes just before hypoxia/reoxygenation is able to prevent the increased expression of inducible NO synthase, therefore reducing NO overproduction. In the present study we investigated the mechanism by which GT reduces NO production. Since a molecular mechanism for polyphenol activity has been postulated, and PPAR activation is related to the transcription of the inducible NO synthase gene, we evaluated the activation of PPAR by GT. A moderate GT concentration, supplemented to the cardiomyocyte medium since the initial seeding, selectively activated the PPAR-β/δ isoform. Furthermore, we observed a reduction in NO production and an increase in total antioxidant activity, indicating that GT components may act on both reactive oxygen species, via an antioxidant mechanism, and NO overproduction. PPAR-β/δ activation could represent the key event in the reduction of NO production by GT. Although PPAR activation by GT was lower than activation by fenofibrate, it is very interesting to note that it was selective for the β/δ isoform, at least in neonatal cardiomyocytes.

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Corresponding author
*Corresponding author: Dr Alessandra Bordoni, fax +39 051 2091235, email alessandra.bordoni@unibo.it
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1GL Tipoe , TM Leung , MW Hung , (2007) Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection. Cardiovasc Hematol Disord Drug Targets 7, 135144.

3V Stangl , H Dreger , K Stangl , (2007) Molecular targets of tea polyphenols in the cardiovascular system. Cardiovasc Res 73, 348358.

5G Agnetti , A Bordoni , C Angeloni , (2005) Green tea modulation of inducible nitric oxide synthase in hypoxic/reoxygenated cardiomyocytes. Biochimie 87, 457460.

6NF Shay & WJ Banz (2005) Regulation of gene transcription by botanicals: novel regulatory mechanisms. Annu Rev Nutr 25, 297315.

7EL Paukkeri , T Leppanen , O Sareila , (2007) PPARα agonists inhibit nitric oxide production by enhancing iNOS degradation in LPS-treated macrophages. Br J Pharmacol 152, 10811091.

8A Bordoni , S Hrelia , C Angeloni , (2002) Green tea protection of hypoxia/reoxygenation injury in cultured cardiac cells. J Nutr Biochem 13, 103111.

10MM Bradford (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72, 248254.

11G Wright , IS Singh , JD Hasday , (2002) Endotoxin stress-response in cardiomyocytes: NF-κB activation and tumor necrosis factor-α expression. Am J Physiol Heart Circ Physiol 282, H872H879.

12R Re , N Pellegrini , A Proteggente , (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26, 12311237.

15H Takano , T Nagai , M Asakawa , (2000) Peroxisome proliferator-activated receptor activators inhibit lipopolysaccharide-induced tumor necrosis factor-α expression in neonatal rat cardiac myocytes. Circ Res 87, 596602.

16A Planavila , R Rodriguez-Calvo , M Jove , (2005) Peroxisome proliferator-activated receptor β/δ activation inhibits hypertrophy in neonatal rat cardiomyocytes. Cardiovasc Res 65, 832841.

17Y Bayon , MA Ortiz , FJ Lopez-Hernandez , (2003) Inhibition of IκB kinase by a new class of retinoid-related anticancer agents that induce apoptosis. Mol Cell Biol 23, 10611074.

18S Nam , DM Smith & QP Dou (2001) Ester bond-containing tea polyphenols potently inhibit proteasome activity in vitro and in vivo. J Biol Chem 276, 1332213330.

19S Hauser , G Adelmant , P Sarraf , (2000) Degradation of the peroxisome proliferator-activated receptor γ is linked to ligand-dependent activation. J Biol Chem 275, 1852718533.

20BI Jugdutt (2002) Nitric oxide and cardioprotection during ischemia–reperfusion. Heart Fail Rev 7, 391405.

21L Sheng , P Ye , YX Liu , (2008) Peroxisome proliferator-activated receptor β/δ activation improves angiotensin II-induced cardiac hypertrophy in vitro. Clin Exp Hypertens 30, 109119.

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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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