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Effect of maternal iron restriction during pregnancy on renal morphology in the adult rat offspring

  • S. J. M. Lisle (a1), R. M. Lewis (a2), C. J. Petry (a2), S. E. Ozanne (a2), C. N. Hales (a2) and A. J. Forhead (a1)...
Abstract

In rats, maternal anaemia during pregnancy causes hypertension in the adult offspring, although the mechanism is unknown. The present study investigated the renal morphology of adult rats born to mothers who were Fe-deficient during pregnancy. Rats were fed either a control (153 mg Fe/kg diet, n 7) or low-Fe (3 mg/kg diet, n 6) diet from 1 week before mating and throughout gestation. At delivery, the Fe-restricted (IR) mothers were anaemic; the IR pups were also anaemic and growth-retarded at 2 d of age. At 3 and 16 months, systolic blood pressure in the IR offspring (163 (sem 4) and 151 (sem 4) mmHg respectively, n 13) was greater than in control animals (145 (sem 3) and 119 (sem 4) mmHg respectively, n 15, P<0·05). At post mortem at 18 months, there was no difference in kidney weight between treatment groups, although relative kidney weight as a fraction of body weight in the IR offspring was greater than in control animals (P<0·05). Glomerular number was lower in the IR offspring (11·4 (sem 1·1) per 4mm2, n 13) compared with control rats (14·8 (sem 0·7), n 15, P<0·05). Maternal treatment had no effect on glomerular size, but overall, female rats had smaller and more numerous glomeruli per unit area than male rats. When all animals were considered, inverse relationships were observed between glomerular number and glomerular size (r−0·73, n 28, P<0·05), and glomerular number and systolic blood pressure at both 3 months (r−0·42, n 28, P<0·05) and 16 months of age (r−0·64, n 28, P<0·05). Therefore, in rats, maternal Fe restriction causes hypertension in the adult offspring that may be due, in part, to a deficit in nephron number.

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Corresponding author
*Corresponding author: Dr Alison J. Forhead, fax +44 1223 333840, email ajf1005@cam.ac.uk
References
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Bains RK, Sibbons PD, Murray RD, Howard CV & Van Velzen D (1996) Stereological estimation of the absolute number of glomeruli in the kidneys of lambs. Res Vet Sci 60, 122125.
Barone A, Harper RG & Wapnir RA (1998) Placental copper transport in the rat. III: Interaction between copper and iron in maternal protein deficiency. Placenta 19, 113118.
Bassan H, Trejo LL, Kariv N, et al. (2000) Experimental intrauterine growth retardation alters renal development. Pediatr Nephrol 15, 192195.
Bertram C, Trowern AR, Copin N, Jackson AA & Whorwood CB (2001) The maternal diet during pregnancy programs altered expression of the glucocorticoid receptor and type 2 11β-hydroxysteroid dehydrogenase: potential molecular mechanisms underlying the programming of hypertension in utero. Endocrinology 142, 28412853.
Brenner BM, Garcia DL & Anderson S (1988) Glomeruli and blood pressure. Less of one, more of the other?. Am J Hypertens 1, 335347.
Campos MS, Barrionuevo M, Alferez MJM, et al. (1998) Interactions among iron, calcium, phosphorus and magnesium in the nutritionally iron-deficient rat. Exp Physiol 83, 771781.
Celsi G, Kistner A, Aizman R, et al. (1998) Prenatal dexamethasone causes oligonephronia, sodium retention, and higher blood pressure in the offspring. Pediatr Res 44, 317322.
Crowe C, Dandekar P, Fox M, Dhingra K, Bennet L & Hanson MA (1995) The effects of anaemia on heart, placenta and body weight, and blood pressure in fetal and neonatal rats. J Physiol 488, 515519.
Doublier S, Amri K, Seurin D, et al. (2001) Overexpression of human insulin-like growth factor binding protein-I in the mouse leads to nephron deficit. Pediatr Res 49, 660666.
Finch CA, Huebers HA, Miller LR, Josephson BM, Shepard TH & Mackler B (1983) Fetal iron balance in the rat. Am J Clin Nutr 37, 910917.
Gambling L, Danzeisen R, Gair S et al. (2001 a) Effect of iron deficiency on placental transfer of iron and expression of iron transport proteins in vivo and in vitro. Biochem J 356, 883889.
Gambling L, Dunford S, Beattie L & Mcardle HJ (2001 b) Postnatal effects of prenatal iron deficiency in the rat. J Physiol 539, 118P, (Abstr).
Hinchliffe SA, Lynch MRJ, Sargent PH, Howard CV & Van Velzen D (1992) The effect of intrauterine growth retardation on the development of renal nephrons. Br J Obstetr Gynaecol 99, 296301.
Hoet JJ & Hanson MA (1999) Intrauterine nutrition: its importance during critical periods for cardiovascular and endocrine development. J Physiol 514, 617627.
Horster MF, Braun GS & Huber SM (1999) Embryonic renal epithelia: induction, nephrogenesis, and cell differentiation. Physiol Rev 79, 11571191.
Jang J-T, Green JB, Beard JL & Green MH (2000) Kinetic analysis shows that iron deficiency decreases liver vitamin A mobilization in rats. J Nutr 130, 12911296.
Langley-Evans SC, Welham SJM & Jackson AA (1999) Fetal exposure to a maternal low protein diet impairs nephrogenesis and promotes hypertension in the rat. Life Sci 64, 965974.
Lelievre-Pegorier M, Vilar J, Ferrier M-L, et al. (1998 a) Mild vitamin A deficiency leads to inborn nephron deficit in the rat. Kidney Int 54, 14551462.
Lelievre-Pegorier M, Vilar J, Freund N, Gilbert T & Merlet-Benichou C (1998 b) Vitamin A prevents nephron deficit in growth-retarded fetal rats. J Am Soc Nephrol 9, 501 Abstr.
Lewis RM, James LA, Zhang J, Byrne CD & Hales CN (2001 a) Effects of maternal iron restriction in the rat on hypoxia-induced gene expression and fetal metabolite levels. Br J Nutr 85, 193201.
Lewis RM, Petry CJ, Ozanne SE, & Hales CN (2001 b) Effects of maternal iron restriction in the rat on blood pressure, glucose tolerance, and serum lipids in the 3-month-old offspring. Metabolism 50, 562567.
Lucas SRR, Miraglia SM, Gil FZ & Coimbra TM (2001) Intrauterine food restriction as a determinant of nephrosclerosis. American Journal of Kidney Diseases 37, 467476.
Manalich R, Reyes L, Herrera M, Melendi C & Fundora I (2000) Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study. Kidney Int 58, 770773.
Merlet-Benichou C, Gilbert T, Muffat-Joly M & Lelievre-Pegorier M (1994) Intrauterine growth retardation leads to a permanent nephron deficit in the rat. Pediatr Nephrol 8, 175180.
Messow C, Gartner K, Hackbarth H, Kangaloo M & Lunebrink L (1980) Sex differences in kidney morphology and glomerular filtration rate in mice. Pediatr Nephrol 19, 5155.
Mosier HD, Spencer EM, Dearden LC & Jansons RA (1987) The effect of glucocorticoids on plasma insulin-like growth factor I concentration in the rat fetus. Pediatr Res 22, 9295.
Rogers SA, Ryan G & Hammerman MR (1991) Insulin-like growth factors I and II are produced in the metanephros and are required for growth and development in vitro. J Cell Biol 113, 14471453.
Rondo PHC, Abbott R, Rodrigues LC & Tomkins AM (1995) Vitamin A, folate, and iron concentrations in cord and maternal blood of intra-uterine growth retarded and appropriate birth weight babies. Eur J Clin Nutr 49, 391399.
Singla PN, Tyagi M, Shankar R, Dash D & Kumar A (1996) Fetal iron status in maternal anemia. Acta Pediatr 85, 13271330.
Tapanainen PJ, Bang P, Wilson K, Unterman TG, Vreman HJ & Rosenfeld RG (1994) Maternal hypoxia as a model for intrauterine growth retardation: effects on insulin-like growth factors and their binding proteins. Pediatr Res 36, 152158.
Tufro-Mcreddie A, Norwood VF, Aylor KW, Botkin SJ, Carey RM & Gomez RA (1997) Oxygen regulates vascular endothelial growth factor-mediated vasculogenesis and tubulogenesis. Dev Biol 183, 139149.
Vaquero MP & Navarro MP (1996) Relationship between moderate food restriction during pregnancy and Fe, Zn and Cu contents in maternal tissues and foetuses. Reprod Nutr Dev 36, 333344.
Vehaskari VM, Aviles DH & Manning J (2001) Prenatal programming of adult hypertension in the rat. Kidney Int 59, 238245.
Wintour EM (1997) The renin-angiotensin system and the development of the kidney. Trends Endocrinol Metab 8, 199207.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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