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Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects

  • S. E. Nielsen (a1), J. F. Young (a2), B. Daneshvar (a1), S. T. Lauridsen (a1), P. Knuthsen (a3), B. Sandström (a2) and L. O. Dragsted (a1)...
Abstract

Seven men and seven women participated in a randomized crossover trial to study the effect of intake of parsley (Petroselinum crispum), containing high levels of the flavone apigenin, on the urinary excretion of flavones and on biomarkers for oxidative stress. The subjects received a strictly controlled diet low in flavones and other naturally occurring antioxidants during the 2 weeks of intervention. This basic diet was supplemented with parsley providing 3·73–4·49 mg apigenin/MJ in one of the intervention weeks. Urinary excretion of apigenin was 1·59–409·09 μg/MJ per 24 h during intervention with parsley and 0–112·27 μg/MJ per 24 h on the basic diet (P < 0·05). The fraction of apigenin intake excreted in the urine was 0·58 (se 0·16) % during parsley intervention. Erythrocyte glutathione reductase (EC 1.6.4.1; GR) and superoxide dismutase (EC 1.15.1.1; SOD) activities increased during intervention with parsley (P < 0·005) as compared with the levels on the basic diet, whereas erythrocyte catalase (EC 1.11.1.6) and glutathione peroxidase (EC 1.11.1.9) activities did not change. No significant changes were observed in plasma protein 2-adipic semialdehyde residues, a biomarker of plasma protein oxidation. In this short-term investigation, an overall decreasing trend in the activity of antioxidant enzymes was observed during the 2-week study. The decreased activity of SOD was strongly correlated at the individual level with an increased oxidative damage to plasma proteins. However, the intervention with parsley seemed, partly, to overcome this decrease and resulted in increased levels of GR and SOD.

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      Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects
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      Effect of parsley (Petroselinum crispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects
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Corresponding author
*Corresponding author: Dr L. O. Dragsted, fax +45 33 95 60 01, email lod@vfd.dk
References
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Ameer B, Weintraub RA, Johnson JV, Yost RA & Rouseff RL (1996) Flavanone absorption after naringin, hesperidin, and citrus administration. Clinical Pharmacology and Therapeutics 60, 3440.
Armitage P & Berry G (1994) Statistical Methods in Medical Research, 3rd ed. Cambridge: Blackwell Science Ltd.
Belsten JL & Wright AJ (1995) European community-FLAIR common assay for erythrocyte superoxide dismutase (SOD); results of an inter-laboratory trial. European Journal of Clinical Nutrition 49, 928931.
Birt DF, Mitchell D, Gold B, Pour P & Pinch HC (1997) Inhibition of ultraviolet light-induced skin carcinogenesis in SKH-1 mice by apigenin, a plant flavonoid. Anticancer Research 17, 8591.
Bro S, Sandström B & Heydorn K (1990) Intake of essential and toxic trace elements in a random sample of Danish men as determined by the duplicate portion sample technique. Journal of Trace Elements and Electrolytes in Health and Disease 4, 147155.
Daneshvar B (1996) Biomarkers for oxidative damage to proteins. Development of analytical methods and their application to biological samples. PhD Thesis, Institute of Pharmacology, University of Copenhagen, Denmark.
Daneshvar B, Frandsen H, Autrup H & Dragsted LO (1997) Gamma-glutamyl semialdehyde and 2-amino-adipic semialdehyde: biomarkers of oxidative damage to proteins. Biomarkers 2, 117123.
Dixon ZR, Burri BJ, Clifford A, Frankel EN, Schneeman BO, Parks E, Keim NL, Barbieri T, Wu MM, Fong AK, Kretsch MJ, Sowell AL & Erdman JW Jr (1994) Effects of α carotene-deficient diet on measures of oxidative susceptibility and superoxide dismutase activity in adult women. Free Radical Biology and Medicine 17, 537544.
Fernandez de Simon B, Perez Ilzarbe J, Hernandez T, Gomez Cordoves C & Estrella I (1992) Importance of phenolic compounds for the characterization of fruit juices. Journal of Agricultural and Food Chemistry 40, 15311535.
Fraga CG, Martino VS, Ferraro GE, Coussio JD & Boveris A (1987) Flavonoids as antioxidants evaluated by in vitro and in situ liver chemiluminescence. Biochemical Pharmacology 36, 717720.
Hart DJ & Scott KJ (1995) Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Food Chemistry 54, 101111.
Hollman PCH, van der Gaag MS, Mengelers MJB, van Trijp JMP, de Vries JHM & Katan MB (1996) Absorption and disposition kinetics of the dietary antioxidant quercetin in man. Free Radical Biology and Medicine 21, 703707.
Hollman PCH, van Trijp JMP, Buysman MNCP, van der Gaag MS, Mengelers MJB, Vries JHM & Katan MB (1997) Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Letters 418, 152156.
Hollman PCH, Vries JHM, van Leeuwen SD, Mengelers MJB & Katan MB (1995) Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers. American Journal of Clinical Nutrition 62, 12761282.
Janssen K, Mensink RP, Cox FJJ, Harryvan JL, Hovenier R, Hollman PCH & Katan MB (1998) Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study. American Journal of Clinical Nutrition 67, 255262.
Justesen U, Knuthsen P & Leth T (1998) Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by HPLC with photodiode array and mass spectrometric detection. Journal of Chromatography A 799, 101110.
Kühnau J (1976) The flavonoids. A class of semi-essential food components: their role in human nutrition. World Review of Nutrition and Dietetics 24, 117191.
Kuo ML, Lee KC & Lin JK (1992) Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, ellagic acid and indole-3-carbinol in the Salmonella and CHO systems. Mutation Research 270, 8795.
Lee SJ, Son KH, Chang HW, Do JC, Jung KY, Kang SS & Kim HP (1993) Antiinflammatory activity of naturally occurring flavone and flavonol glycosides. Archives of Pharmacology Research 16, 2528.
Lux O & Naidoo D (1995) Biological variability of superoxide dismutase and glutathione peroxidase in blood. Redox Report 1, 331335.
Milne DB & Nielsen FH (1996) Effects of a diet low in copper on copper-status indicators in postmenopausal women. American Journal of Clinical Nutrition 63, 358364.
Møller A (1996) Levnedsmiddeltabeller (The Composition of Foods), 4th revised and extended ed. [Saxholt E, editor]. Copenhagen: National Food Agency of Denmark.
Nielsen SE & Dragsted LO (1998) Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection. Journal of Chromatography B 713, 379386.
Pocock SJ (1998) Clinical trials. A practical approach. Chichester: John Wiley & Sons.
Saghir AN, Rickards H & Pall HS (1997) Postprandial changes in superoxide dismutase activity in subjects with Gilles de la Tourette syndrome and controls. Experimental Neurology 144, 420422.
Tschiersch K & Hölzl J (1993) Resorption und Ausscheidung von Apigenin, Apigenin-7-glucosid und Herniarin nach Peroraler Gabe eines Extract von Matricaria recutita (L.) [syn. Chamomilla recutita (L.) Rauschert] (Absorption and excretion of apigenin, apigenin-7-glycoside and herniarin after oral administration of extracts of Matricaria recutita). Pharmazie 48, 554555.
van Acker SABE, van den Berg DJ, Tromp MNJL, Griffioen DH, van Bennekom WP, van der Vijgh WJF & Bast A (1996) Structural aspects of antioxidant activity of flavonoids. Free Radicical Biology and Medicine 20, 331342.
Wei H, Tye L, Bresnick E & Birt DF (1989) Inhibitory effect of apigenin, a plant flavonoid, on epidermal ornithine decarboxylase and skin tumor promotion in mice. Cancer Research 50, 499502.
Wheeler CR, Salzman JA, Elsayed NM, Omaye ST & Korte DW Jr (1990) Automated assay for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase. Analytical Biochemistry 184, 193199.
Young JF, Nielsen SE, Haraldsdóttir J, Daneshvar B, Lauridsen ST, Knuthsen P, Crozier A, Sandström B & Dragsted LO (1999) Effect of juice intake on urinary quercetin excretion and biomarkers of antioxidative status. American Journal of Clinical Nutrition 69, 8794.
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British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
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