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The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects

Published online by Cambridge University Press:  09 March 2007

Colin D. Kay
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
Bruce J. Holub*
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada
*
*Corresponding author: Dr Bruce J. Holub, fax +1 519 763 5902, email bhoub@uoguelph.ca
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Abstract

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The aim of the present study was to determine whether the consumption of wild blueberries (Vaccinium angustifolium), a concentrated source of non-nutritive antioxidant phytochemicals, would enhance postprandial serum antioxidant status in healthy human subjects. A single-blinded crossover study was performed in a group of eight middle-aged male subjects (38–54 years). Subjects consumed a high-fat meal and a control supplement followed 1 week later by the same high-fat meal supplemented with 100·0 g freeze-dried wild blueberry powder. Upon brachial vein catheterization, fasting and postprandial serum samples were taken sequentially and analysed for lipids and glucose and for serum antioxidant status. Serum antioxidant status was determined using the oxygen radical absorbance capacity (ORAC) assay and the total antioxidant status (TAS) assay. The wild-blueberry treatment was associated with a significant treatment effect as determined by the ORAC assay (water-soluble fraction ORACperchloric acid (PCA), P=0·04). Significant increases in serum antioxidant status above the controls were observed at 1 h (ORACPCA (8·5 % greater), P=0·02; TAS (4·5 % greater), P=0·05), and 4 h (ORACtotal (15·0 % greater), P=0·009; ORACacetone (16·0 % greater), P=0·007) post-consumption of the high-fat meal. In conclusion, the consumption of wild blueberries, a food source with high in vitro antioxidant properties, is associated with a diet-induced increase in ex vivo serum antioxidant status. It has been suggested that increasing the antioxidant status of serum may result in the reduced risk of many chronic degenerative diseases.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2002

References

Ames, BN, Gold, LS & Willett, WC (1995) The causes and prevention of cancer. Proceedings of the National Academy of Sciences USA 92, 52585265.CrossRefGoogle ScholarPubMed
Bushway, RB, McGann, DF, Cook, WP & Bushway, AA (1983) Mineral and vitamin content of lowbush blueberries (Vaccinium angustifolium). Journal of Food Science 48, 18781880.CrossRefGoogle Scholar
Cao, G, Alessio, HM & Cutler, RG (1993) Oxygen-radical absorbance capacity assay for antioxidants. Free Radical Biology and Medicine 14, 303311.CrossRefGoogle ScholarPubMed
Cao, G, Muccitelli, HU, Sánchez-Moreno, C & Prior, RL (2001) Anthocyanins are absorbed in glycated forms in elderly women: a pharmacokinetic study. American Journal of Clinical Nutrition 73, 920926.CrossRefGoogle ScholarPubMed
Cao, G & Prior, RL (1998) Comparison of different analytical methods for assessing total antioxidant capacity of human serum. American Journal of Clinical Nutrition 44, 13091315.Google ScholarPubMed
Cao, G & Prior, RL (2000) Postprandial increases in serum antioxidant capacity in older women. Journal of Applied Physiology 89, 877883.CrossRefGoogle ScholarPubMed
Castelli, WP (1998) The new pathophysiology of coronary artery disease. American Journal of Cardiology 82, 60T65T.CrossRefGoogle ScholarPubMed
Chandramouli, V, Kumaran, K, Ekberg, K, Wahren, J & Landau, BR (1993) Quantitation of the pathways followed in the conversion of fructose to glucose in liver. Metabolism 42, 14201423.CrossRefGoogle ScholarPubMed
Chieas, R, Melissano, G, Castellano, R, Astore, D, Marone, EM, Grossi, A, Maggi, E, Finardi, G, Casac, A, Bellonmo, G & Pavia, M (1998) In search of biological markers of high-risk carotid artery atherosclerotic plaque: enhanced LDL oxidation. Annals of Vascular Surgery 12, 19.CrossRefGoogle Scholar
Ching, S, Ingram, D, Hahnel, R, Beilby, J & Rossi, E (2002) Serum levels of micronutrients, antioxidants and total antioxidant status predict risk of breast cancer in a case control study. Journal of Nutrition 132, 303306.CrossRefGoogle ScholarPubMed
Crews, W, Alink, G, Anderson, R, Braesco, V, Holst, B, Maiani, G, Ovesen, L, Scotter, M, Solfizzo, M, van den Berg, H, Verhagen, H & Williamson, G (2001) A critical assessment of some biomarker approaches linked with dietary intake. British Journal of Nutrition 86, S5S35.CrossRefGoogle ScholarPubMed
Diplock, AT, Charleux, JL, Grozier-Willi, G, Kok, FJ, Rice-Evans, C, Roberfroid, M, Stahl, W & Vina-Ribes, J (1998) Functional food science and defence against reactive oxidative species. British Journal of Nutrition 80, S77S112.CrossRefGoogle ScholarPubMed
Durak, II, Kacmaz, M, Cimen, MY, Buyukkock, U & Ozturk, HS (2001) Blood oxidant/antioxidant status of atherosclerotic patients. International Journal of Cardiology 77, 293297.CrossRefGoogle ScholarPubMed
Francis, FJ (1989) Food colorants: anthocyanins. Critical Reviews in Food Science and Nutrition 28, 273314.CrossRefGoogle ScholarPubMed
Friedewald, WT, Levy, RI & Fredrikson, DS (1972) Estimation of the concentration of low-density-lipoprotein cholesterol in plants without the use of preparative ultracentrifuge. Clinical Chemistry 18, 499502.Google Scholar
Georgopoulos, A (1999) Postprandial triglyceride metabolism in diabetes mellitus. Clinical Cardiology 22, 28S33S.CrossRefGoogle ScholarPubMed
Hennig, B, Toborek, M & McClain, CJ (2001) High-energy diets, fatty acids and endothelial cell function: implications for atherosclerosis. Journal of the American College of Nutrition 20, 97S105S.CrossRefGoogle ScholarPubMed
Hertog, MGL, Hollman, PCH, Katan, MB & Kromhout, D (1993) Intake of potentially anticarcinogenic flavonoids and their determinants in adults in the Netherlands. Nutrition and Cancer 20, 2129.CrossRefGoogle ScholarPubMed
Howarth, NC, Saltzman, E & Roberts, SB (2001) Dietary fibre and weight regulation. Nutrition Reviews 59, 129139.CrossRefGoogle ScholarPubMed
Kalt, W, Forney, CF, Martin, A & Prior, RL (1999) Antioxidant capacity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits. Journal of Agriculture and Food Chemistry 47, 46384644.CrossRefGoogle ScholarPubMed
Kaplan, M & Aviram, M (1999) Oxidized low density lipoprotein: Atherogenic and proinflammatory characteristics during macrophage foam cell formation. An inhibitory role for nutritional antioxidants and serum paraoxonase. Clinical Chemistry and Laboratory Medicine 37, 777787.CrossRefGoogle ScholarPubMed
Lachance, PA (1998) Overview of key nutrients: macronutrient aspects. Nutrition Reviews 56, S34S39.CrossRefGoogle ScholarPubMed
Laplaud, PM, Lelubre, A & Chapman, MJ (1997) Antioxidant action of Vaccinium myrtillus extract on human low density lipoproteins in vitro: initial observations. Fundamentals of Clinical Pharmacology 11, 3540.CrossRefGoogle ScholarPubMed
Liu, D, Manson, JE, Lee, I, Cole, SR, Hennekens, CH, Willet, WC & Buring, JE (2000) Fruit and vegetable intake and risk of cardiovascular disease: the women's health study. American Journal of Clinical Nutrition 72, 922928.CrossRefGoogle ScholarPubMed
Liu, Q, Scheller, KK & Schaefer, DM (1996) Technical note: A simplified procedure for vitamin E determination in beef muscle. Journal of Animal Science 74, 24062410.CrossRefGoogle ScholarPubMed
Loft, S & Poulsen, HE (1996) Cancer risk and oxidative DNA damage in man. Journal of Molecular Medicine 74, 297312.CrossRefGoogle ScholarPubMed
Mazza, B & Oomah, BO (2000) Herbs, Botanicals and Teas, pp. 289318 [Mazza, G and Oomah, BO, editors]. Lancaster, Basel, USA: Technomic Publishing Co. Inc.Google Scholar
Middleton, E, Kandaswami, C & Theoharides, TC (2000) The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacological Reviews 52, 673751.Google ScholarPubMed
Niki, E (1996) Alpha-tocopherol. In Handbook of Antioxidants, pp. 325 [Cadenas, E and Parker, L, editors]. New York: Marcel Dekker.Google Scholar
Ou, S, Kwok, K, Li, Y & Fu, L (2001) In vitro study of possible role of dietary fiber in lowering postprandial serum glucose. Journal of Agricultural and Food Chemistry 42, 10261029.CrossRefGoogle Scholar
Papas, AM (1996) Determinants of antioxidant status in humans. Lipids 31, 77S82S.CrossRefGoogle ScholarPubMed
Payne, TJ (2000) Cultivated Blueberry Composition. North American Blueberry Council. http://www.blueberry.org/composit.htmlGoogle Scholar
Perman, JA (1996) Digestion and absorption of fruit juice carbohydrates. Journal of the American College of Nutrition 15, 12S17S.CrossRefGoogle ScholarPubMed
Priemé, H, Loft, S, Nyyssönen, K, Salonen, JT & Poulsen, HE (1997) No effect of supplementation with vitamin E, ascorbic acid, or coenzyme Q10 on oxidative damage estimated by 8-oxo-7,8-dihydro-2'-deoxyguanosine excretion in smokers. American Journal of Clinical Nutrition 65, 503507.CrossRefGoogle ScholarPubMed
Prior, RL, Cao, G, Martin, A, Sofic, E, McEwan, J, O'Brein, C, Lischner, N, Ehlenfeldt, M, Kalt, W, Krewer, G & Mainland, CM (1998) Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity and variety of Vaccinium species. Journal of Agriculture and Food Chemistry 46, 26862693.CrossRefGoogle Scholar
Rice-Evans, C (2001) Flavonoid antioxidants. Current Medicinal Chemistry 8, 797807.CrossRefGoogle ScholarPubMed
Ryu, JE, Howard, G, Craven, TE, Bond, TE, Hagaman, AP & Crose, JR (1992) Postprandial triglyceridemia and carotid atherosclerosis in middle aged subjects. Stroke 23, 823828.CrossRefGoogle ScholarPubMed
Salonen, JT, Nyyssonen, K, Salonin, R, Lakka, HM, Kaikkonen, J, Porkkala-Saratatho, E, Voutilainen, S, Lakka, TA, Rissanen, T, Leskinen, L, Tuomainen, TP, Valkonen, VP, Voutilainen, S & Poulsen, HE (2000) Antioxidant supplementation in atherosclerosis prevention (ASAP) study: a randomized trial of the effects of vitamin E and C on 3-year progression of carotid atherosclerosis. Journal of Internal Medicine 248, 377386.CrossRefGoogle Scholar
Satué-Gracia, T, Heinonen, M & Frankel, E (1997) Anthocyanins as antioxidants on human low-density liposome systems. Journal of Agriculture and Food Chemistry 45, 33623367.CrossRefGoogle Scholar
Scalbert, A & Williamson, G (2000) Dietary intake and bioavailability of polyphenols. Journal of Nutrition 130, 2073S2085S.CrossRefGoogle ScholarPubMed
Scheppach, W, Luehrs, H & Menzel, T (2001) Beneficial health effects of low-digestible carbohydrate consumption. British Journal of Nutrition 85, S23S30.CrossRefGoogle ScholarPubMed
Shi, X, Schedl, HP, Summers, RM, Lambert, GP, Chang, R, Xia, T & Gisolf, CV (1997) Fructose transport mechanisms in humans. Gastroenterology 113, 11711179.CrossRefGoogle ScholarPubMed
Speek, AJ, Schrijver, J & Schreurs, WHP (1984) Fluorimetric detection of total vitamin C and total vitamin iso-C in foodstuff and beverages by high performance liquid chromatography with pre-column derivatization. Journal of Agriculture and Food Chemistry 32, 352355.CrossRefGoogle Scholar
Steinmetz, KA & Potter, JD (1996) Vegetables, fruit, and cancer prevention: A review. Journal of the American Dietetic Association 96, 10271039.CrossRefGoogle ScholarPubMed
van den Berg, R, Van Vliet, T, Broekmans, WMR, Cnubben, NHP, Vaes, WHJ, Roza, L, Haenen, GRM, Bast, A & van den Berg, H (2001) A vegetable/fruit concentrate with high antioxidant capacity has no effect on biomarkers of antioxidant status in male smokers. Journal of Nutrition 131, 17141722.CrossRefGoogle ScholarPubMed
Vendemiale, G, Grattagliano, I & Altomare, E (1999) An update on the role of free radicals and antioxidant defense in human disease. International Journal of Clinical and Laboratory Research 29, 4955.CrossRefGoogle ScholarPubMed
Wang, H, Cao, G & Prior, R (1996) Total antioxidant capacity of fruits. Journal of Agriculture and Food Chemistry 44, 701705.CrossRefGoogle Scholar
Wang, SY & Lin, H (2000) Antioxidant activity in fruits and leaves of blackberry, raspberry and strawberry varies with cultivar and developmental stage. Journal of Agriculture and Food Chemistry 48, 140146.CrossRefGoogle ScholarPubMed
Willett, WC (2001) Diet and cancer: One view at the start of the millennium. Cancer Epidemiology Biomarkers and Prevention 10, 38.Google ScholarPubMed
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