Skip to main content
    • Aa
    • Aa
  • Access
  • Cited by 47
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Domínguez-Avila, J. González-Aguilar, Gustavo Alvarez-Parrilla, Emilio and de la Rosa, Laura 2016. Modulation of PPAR Expression and Activity in Response to Polyphenolic Compounds in High Fat Diets. International Journal of Molecular Sciences, Vol. 17, Issue. 7, p. 1002.

    El-Newary, Samah Ali 2016. The hypolipidemic effect of Portulaca oleracea L. stem on hyperlipidemic Wister Albino rats. Annals of Agricultural Sciences, Vol. 61, Issue. 1, p. 111.

    Jagtap, Sneha Khare, Pragyanshu Mangal, Priyanka Kondepudi, Kanthi Kiran Bishnoi, Mahendra and Bhutani, Kamlesh Kumar 2016. Protective effects of phyllanthin, a lignan from Phyllanthus amarus, against progression of high fat diet induced metabolic disturbances in mice. RSC Adv., Vol. 6, Issue. 63, p. 58343.

    Shafie, Siti Raihanah Poudyal, Hemant Panchal, Sunil K. and Brown, Lindsay 2016. Omega-3 Fatty Acids.

    Sun, Jian Tang, Yuhan Yu, Xiao Xu, Yanyan Liu, Peiyi Xiao, Lin Liu, Liegang Deng, Qianchun and Yao, Ping 2016. Flaxseed lignans alleviate high fat diet-induced hepatic steatosis and insulin resistance in mice: Potential involvement of AMP-activated protein kinase. Journal of Functional Foods, Vol. 24, p. 482.

    Tamura, Motoi Hori, Sachiko Nakagawa, Hiroyuki Yamauchi, Satoshi and Sugahara, Takuya 2016. Effects of an equol-producing bacterium isolated from human faeces on isoflavone and lignan metabolism in mice. Journal of the Science of Food and Agriculture, Vol. 96, Issue. 9, p. 3126.

    Cassani, Roberta Soares Fassini, Priscila Silvah, Jose Lima, Cristiane Maria and Marchini, Júlio 2015. Impact of weight loss diet associated with flaxseed on inflammatory markers in men with cardiovascular risk factors: a clinical study. Nutrition Journal, Vol. 14, Issue. 1, p. 5.

    De Marchi, F.E. Palin, M.-F. dos Santos, G.T. Lima, L.S. Benchaar, C. and Petit, H.V. 2015. Flax meal supplementation on the activity of antioxidant enzymes and the expression of oxidative stress- and lipogenic-related genes in dairy cows infused with sunflower oil in the abomasum. Animal Feed Science and Technology, Vol. 199, p. 41.

    Hu, Yang Song, Yan Franke, Adrian A. Hu, Frank B. van Dam, Rob M. and Sun, Qi 2015. A Prospective Investigation of the Association Between Urinary Excretion of Dietary Lignan Metabolites and Weight Change in US Women. American Journal of Epidemiology, Vol. 182, Issue. 6, p. 503.

    Imran, Muhammad Ahmad, Nazir Anjum, Faqir Muhammad Khan, Muhammad Kamran Mushtaq, Zarina Nadeem, Muhammad and Hussain, Shahzad 2015. Potential protective properties of flax lignan secoisolariciresinol diglucoside. Nutrition Journal, Vol. 14, Issue. 1,

    Wang, Yanwen Fofana, Bourlaye Roy, Moumita Ghose, Kaushik Yao, Xing-Hai Nixon, Marva-Sweeney Nair, Sandhya and Nyomba, Gregoire B.L. 2015. Flaxseed lignan secoisolariciresinol diglucoside improves insulin sensitivity through upregulation of GLUT4 expression in diet-induced obese mice. Journal of Functional Foods, Vol. 18, p. 1.

    Barrena, Helenton C. Schiavon, Fabiana P. M. Cararra, Marcia A. Marques, Any de Castro R. Schamber, Christiano R. Curi, Rui and Bazotte, Roberto B. 2014. Effect of linseed oil and macadamia oil on metabolic changes induced by high-fat diet in mice. Cell Biochemistry and Function, Vol. 32, Issue. 4, p. 333.

    Biasiotto, Giorgio Penza, Marialetizia Zanella, Isabella Cadei, Moris Caimi, Luigi Rossini, Cristina Smeds, Annika I. and Di Lorenzo, Diego 2014. Oilseeds ameliorate metabolic parameters in male mice, while contained lignans inhibit 3T3-L1 adipocyte differentiation in vitro. European Journal of Nutrition, Vol. 53, Issue. 8, p. 1685.

    Correia-Santos, André Manoel Suzuki, Akemi Vicente, Gabriela Câmara dos Anjos, Juliana Saraiva Pereira, Aline D’Avila Lenzi-Almeida, Kátia Calvi and Boaventura, Gilson Teles 2014. Effect of maternal use of flaxseed oil during pregnancy and lactation on glucose metabolism and pancreas histomorphometry of male offspring from diabetic rats. Diabetes Research and Clinical Practice, Vol. 106, Issue. 3, p. 634.

    Li, Xiaofan Li, Ling Wang, Jue Wang, Tiejie and Wang, Liyan 2014. Two new lignans with antioxidative activities fromJatropha curcas. Natural Product Research, Vol. 28, Issue. 22, p. 1985.

    Park, Jae B. 2014. Nutrition in the Prevention and Treatment of Abdominal Obesity.

    Shim, Youn Young Gui, Bo Arnison, Paul G. Wang, Yong and Reaney, Martin J.T. 2014. Flaxseed (Linum usitatissimum L.) bioactive compounds and peptide nomenclature: A review. Trends in Food Science & Technology, Vol. 38, Issue. 1, p. 5.

    Spilioti, Eliana Holmbom, Bjarne Papavassiliou, Athanasios G. and Moutsatsou, Paraskevi 2014. Lignans 7-hydroxymatairesinol and 7-hydroxymatairesinol 2 exhibit anti-inflammatory activity in human aortic endothelial cells. Molecular Nutrition & Food Research, Vol. 58, Issue. 4, p. 749.

    Sun, Qi Wedick, Nicole M. Pan, An Townsend, Mary K. Cassidy, Aedin Franke, Adrian A. Rimm, Eric B. Hu, Frank B. and van Dam, Rob M. 2014. Gut Microbiota Metabolites of Dietary Lignans and Risk of Type 2 Diabetes: A Prospective Investigation in Two Cohorts of U.S. Women. Diabetes Care, Vol. 37, Issue. 5, p. 1287.

    Abdelkarem, Hala M. and Fadda, Lila H. 2013. Flaxseed and quercetin improve anti-inflammatory cytokine level and insulin sensitivity in animal model of metabolic syndrome, the fructose-fed rats. Arabian Journal of Chemistry,


Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice

  • S. Fukumitsu (a1), K. Aida (a1), N. Ueno (a1), S. Ozawa (a2), Y. Takahashi (a3) and M. Kobori (a3)
  • DOI:
  • Published online: 01 September 2008

Flaxseed lignan secoisolariciresinol diglucoside (SDG) has been reported to prevent and alleviate lifestyle-related diseases including diabetes and hypercholesterolaemic atherosclerosis. This study assesses the effect of SDG on the development of diet-induced obesity in mice and the effect of the SDG metabolite enterodiol (END) on adipogenesis in 3T3-L1 adipocytes. We compared body weight, visceral fat weight, liver fat content, serum parameters, mRNA levels of lipid metabolism-related enzymes and adiponectin in mice fed either a low-fat diet (5 % TAG), high-fat diet (30 % TAG) or high-fat diet containing 0·5 and 1·0 % (w/w) SDG for 4 weeks. Administration of SDG to mice significantly reduced high-fat diet-induced visceral and liver fat accumulation, hyperlipaemia, hypercholesterolaemia, hyperinsulinaemia and hyperleptinaemia. SDG also suppressed sterol regulatory element binding protein 1c mRNA level in the liver and induced increases in the adiponectin mRNA level in the white adipose tissue and carnitine palmitoyltransferase I mRNA level in the skeletal muscle. Differentiated 3T3-L1 adipocytes were treated with 0, 5, 10 and 20 μmol/l END and then assayed for mRNA expression of adipogenesis-related genes and DNA binding activity of PPARγ to the PPAR response element consensus sequence. END induced adipogenesis-related gene mRNA expression including adiponectin, leptin, glucose transporter 4 and PPARγ, and induced PPARγ DNA binding activity in 3T3-L1 adipocytes. In conclusion, SDG induced adiponectin mRNA expression and showed beneficial effects on lipid metabolism in diet-induced obesity in mice. Flaxseed lignans are suggested to regulate adipogenesis-related gene expressions through an increase in PPARγ DNA binding activity.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the or variations. ‘’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Flaxseed lignan attenuates high-fat diet-induced fat accumulation and induces adiponectin expression in mice
      Available formats
Corresponding author
*Corresponding author: Dr Masuko Kobori, fax +81 29 838 7996, email
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1MJ Pagliassotti , EC Gayles & JO Hill (1997) Fat and energy balance. Ann N Y Acad Sci 827, 431448.

2J Triscari , C Nauss-Karol , BE Levin & AC Sullivan (1985) Changes in lipid metabolism in diet-induced obesity. Metabolism 34, 580587.

4LU Thompson , SE Rickard , F Cheung , EO Kenaschuk & WR Obermeyer (1997) Variability in anticancer lignan levels in flaxseed. Nutr Cancer 27, 2630.

5SC Cunnane , S Ganguli , C Menard , (1993) High alpha-linolenic acid flaxseed (Linum usitatissimum): some nutritional properties in humans. Br J Nutr 69, 443453.

6JR Romans , RC Johnson , DM Wulf , GW Libal & WJ Costello (1995) Effects of ground flaxseed in swine diets on pig performance and on physical and sensory characteristics and omega-3 fatty acid content of pork: I. Dietary level of flaxseed. J Anim Sci 73, 19821986.

7M Axelson , J Sjovall , BE Gustafsson & KD Setchell (1982) Origin of lignans in mammals and identification of a precursor from plants. Nature 298, 659660.

9L Wang , J Chen & LU Thompson (2005) The inhibitory effect of flaxseed on the growth and metastasis of estrogen receptor negative human breast cancer xenografts is attributed to both its lignan and oil components. Int J Cancer 116, 793798.

10K Prasad (1997) Hydroxyl radical-scavenging property of secoisolariciresinol diglucoside (SDG) isolated from flax-seed. Mol Cell Biochem 168, 117123.

11K Prasad (1999) Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99, 13551362.

12D Li , JA Yee , LU Thompson & L Yan (1999) Dietary supplementation with secoisolariciresinol diglycoside (SDG) reduces experimental metastasis of melanoma cells in mice. Cancer Lett 142, 9196.

13K Prasad (2001) Secoisolariciresinol diglucoside from flaxseed delays the development of type 2 diabetes in Zucker rat. J Lab Clin Med 138, 3239.

14K Prasad , SV Mantha , AD Muir & ND Westcott (2000) Protective effect of secoisolariciresinol diglucoside against streptozotocin-induced diabetes and its mechanism. Mol Cell Biochem 206, 141149.

16Y Matsuzawa , T Funahashi & T Nakamura (1999) Molecular mechanism of metabolic syndrome X: contribution of adipocytokines adipocyte-derived bioactive substances. Ann N Y Acad Sci 892, 146154.

17T Yamauchi , J Kamon , H Waki , (2001) The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 7, 941946.

18P Johnsson , A Kamal-Eldin , LN Lundgren & P Aman (2000) HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds. J Agric Food Chem 48, 52165219.

19J Fritsche , R Angoelal & M Dachtler (2002) On-line liquid-chromatography-nuclear magnetic resonance spectroscopy-mass spectrometry coupling for the separation and characterization of secoisolariciresinol diglucoside isomers in flaxseed. J Chromatogr 972, 195203.

21T Shoji , M Kobori , H Shinmoto , A Yanagida , T Kanda & T Tsushida (2000) Inhibitory effects of apple polyphenols on differentiation of 3T3-L1 cells into adipocytes. Food Sci Technol Res 6, 119121.

22DJ Dyck , GJ Heigenhauser & CR Bruce (2006) The role of adipokines as regulators of skeletal muscle fatty acid metabolism and insulin sensitivity. Acta Physiol (Oxf) 186, 516.

23M Armoni , N Kritz , C Harel , F Bar-Yoseph , H Chen , MJ Quon & E Karnieli (2003) Peroxisome proliferator-activated receptor-gamma represses GLUT4 promoter activity in primary adipocytes, and rosiglitazone alleviates this effect. J Biol Chem 278, 3061430623.

24AN Hollenberg , VS Susulic , JP Madura , B Zhang , DE Moller , P Tontonoz , P Sarraf , BM Spiegelman & BB Lowell (1997) Functional antagonism between CCAAT/enhancer binding protein-alpha and peroxisome proliferator-activated receptor-gamma on the leptin promoter. J Biol Chem 272, 52835290.

25M Iwaki , M Matsuda , N Maeda , T Funahashi , Y Matsuzawa , M Makishima & I Shimomura (2003) Induction of adiponectin, a fat-derived antidiabetic and antiatherogenic factor, by nuclear receptors. Diabetes 52, 16551663.

26BP Kota , TH Huang & BD Roufogalis (2005) An overview on biological mechanisms of PPARs. Pharmacol Res 51, 8594.

27N Marx , H Duez , JC Fruchart & B Staels (2004) Peroxisome proliferator-activated receptors and atherogenesis: regulators of gene expression in vascular cells. Circ Res 94, 11681178.

30SJ Bhathena , AA Ali , C Haudenschild , (2003) Dietary flaxseed meal is more protective than soy protein concentrate against hypertriglyceridemia and steatosis of liver in an animal model of obesity. J Am Coll Nutr 22, 157164.

31HJ Kim , M Takahashi & O Ezaki (1999) Fish oil feeding decreases mature sterol regulatory element-binding protein 1 (SREBP-1) by down-regulation of SREBP-1c mRNA in mouse liver. A possible mechanism for down-regulation of lipogenic enzyme mRNAs. J Biol Chem 274, 2589225898.

32H Fujita , H Fujishima , J Koshimura , M Hosoba , N Yoshioka , T Shimotomai , T Morii , T Narita , M Kakei & S Ito (2005) Effects of antidiabetic treatment with metformin and insulin on serum and adipose tissue adiponectin levels in db/db mice. Endocr J 52, 427433.

33NF Brown , JK Hill , V Esser , JL Kirkland , BE Corkey , DW Foster & JD McGarry (1997) Mouse white adipocytes and 3T3-L1 cells display an anomalous pattern of carnitine palmitoyltransferase (CPT) I isoform expression during differentiation. Inter-tissue and inter-species expression of CPT I and CPT II enzymes. Biochem J 327, Pt 1, 225231.

35T Clavel , G Henderson , W Engst , J Dore & M Blaut (2006) Phylogeny of human intestinal bacteria that activate the dietary lignan secoisolariciresinol diglucoside. FEMS Microbiol Ecol 55, 471478.

36JM Lehmann , LB Moore , TA Smith-Oliver , WO Wilkison , TM Willson & SA Kliewer (1995) An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). J Biol Chem 270, 1295312956.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *