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High concordance of daidzein-metabolizing phenotypes in individuals measured 1 to 3 years apart

  • Cara L. Frankenfeld (a1) (a2), Charlotte Atkinson (a1), Wendy K. Thomas (a1), Alex Gonzalez (a1) (a3), Tuija Jokela (a4), Kristiina Wähälä (a4), Stephen M. Schwartz (a2) (a5), Shuying S. Li (a1) and Johanna W. Lampe (a1) (a2) (a3)
  • DOI:
  • Published online: 01 March 2007

Particular intestinal bacteria are capable of metabolizing the soya isoflavone daidzein to equol and/or O-desmethylangolensin (O-DMA), and the presence of these metabolites in urine after soya consumption are markers of particular intestinal bacteria profiles. Prevalences of equol producers and O-DMA producers are approximately 30–50 % and 80–90 %, respectively, and limited observations have suggested that these daidzein-metabolizing phenotypes are stable within individuals over time. Characterizing stability of these phenotypes is important to understand their potential as markers of long-term exposure to particular intestinal bacteria and their associations with disease risk. We evaluated concordance within an individual for the equol-producer and O-DMA-producer phenotypes measured at two time points (T1, T2), 1–3 years apart. Phenotypes were ascertained by analysing equol and O-DMA using GC-MS in a spot urine sample collected after 3 d soya (source of daidzein) supplementation. In ninety-two individuals without recent (within 3 months before phenotyping) or current antibiotics use, 41 % were equol producers at T1 and 45 % were equol producers at T2, and 90 % were O-DMA producers at T1 and 95 % were O-DMA producers at T2. The percentage agreement for the equol-producer phenotype was 82 and for the O-DMA-producer phenotype was 89. These results indicate that these phenotypes are stable in most individuals over time, suggesting that they provide a useful biomarker for evaluating disease risk associated with harbouring particular intestinal bacteria responsible for, or associated with, the metabolism of the soya isoflavone daidzein.

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*Corresponding author: Dr Johanna W. Lampe, fax +1 206 667 7850, email
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H Adlercreutz , F Martin , M Pulkkinen , H Dencker , U Rimer , N-O Sjöberg & MJ Tikkanen (1976) Intestinal metabolism of estrogens. J Clin Endocrinol Metab 43, 497505.

H Akaza , N Miyanaga , N Takashima , (2004) Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents. Jpn J Clin Oncol 34, 8689.

E Bowey , H Adlercreutz & I Rowland (2003) Metabolism of isoflavones and lignans by the gut microflora: a study in germ-free and human flora associated rats. Food Chem Toxicol 41, 631636.

Y-C Chang & MG Nair (1995) Metabolism of daidzein and genistein by intestinal bacteria. J Nat Prod 58, 18921896.

CF Favier , EE Vaughan , WM De Vos & AD Akkermans (2002) Molecular monitoring of succession of bacterial communities in human neonates. Appl Environ Microbiol 68, 219226.

CL Frankenfeld , A McTiernan & SS Tworoger (2004b) Serum steroid hormones, sex hormone-binding globulin concentrations, and urinary hydroxylated estrogen metabolites in post-menopausal women in relation to daidzein-metabolizing phenotypes. J Steroid Biochem Mol Biol 88, 399408.

S Heinonen , K Wähälä & H Adlercreutz (1999) Identification of isoflavone metabolites dihydrodaidzein, dihydrogenistein, 6'-OH-O-DMA, and cis-4-OH-equol in human urine by gas chromatography–mass spectroscopy using authentic reference compounds. Anal Biochem 274, 211219.

P Järvenpää , T Kosunen , T Fotsis & H Adlercreutz (1980) In vitro metabolism of estrogens by isolated intestinal micro-organisms and by human faecal microflora. J Steroid Biochem 13, 345349.

P Lombardi , B Goldin , E Boutin & S Gorbach (1978) Metabolism of androgens and estrogens by human fecal microorganisms. J Steroid Biochem 9, 795801.

WD Thompson & SD Walter (1988) A reappraisal of the kappa coefficient. J Clin Epidemiol 41, 949958.

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