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British Journal of Nutrition British Journal of Nutrition

Article contents

Ileal pH and apparent absorption of magnesium in rats fed on diets containing either lactose or lactulose

Published online by Cambridge University Press:  02 April 2007

Astrid M. P. Heijnen ,
Elizabeth J. Brink ,
Arnoldina G. Lemmens  and
Anton C. Beynen
Astrid M. P. Heijnen
Affiliation:
Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands Department of Human Nutrition, Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
Elizabeth J. Brink
Affiliation:
Department of Nutrition, Netherlands Institute for Dairy Science, PO Box 20, 6710 BA Ede, The Netherlands
Arnoldina G. Lemmens
Affiliation:
Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands
Anton C. Beynen
Affiliation:
Department of Laboratory Animal Science, State University, PO Box 80.166, 3508 TD Utrecht, The Netherlands Department of Human Nutrition, Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands
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Abstract

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The hypothesis was tested that dietary lactose v. glucose stimulates Mg absorption in rats because lactose lowers pH of the ileal lumen, which improves Mg solubility which in turn enhances Mg availability for transport across the ileal epithelium. For comparison, the effects of lactulose were studied because it shares with lactose the characteristic of being poorly digestible. Replacement of glucose by lactose (100 g/Kg) significantly stimulated apparent absorption of Mg. Apart from Mg absorption, lactulose also significantly enhanced absorption of Ca and phosphate. Lactose v. glucose lowered the pH of the ileal lumen from 7·5 to 7·2, whereas lactulose significantly reduced it to 7·0. In in vitro incubations a decrease in pH within the range of fluctuation in vivo was found to cause an improved solubility of Mg, and to a lesser extent also of Ca and phosphate. The smaller fall of ileal pH induced by feeding lactose instead of lactulose may explain why lactose improved Mg absorption only. For all individual rats combined there were negative relationships between ileal pH and apparent absorption of minerals, the relationship being strongest for Mg. Neither lactose nor lactulose was found to raise ileal solubility of minerals, which could relate to the possibility that the time of sampling was not appropriate. It is suggested that lactose-induced stimulation of Mg absorption in rats is caused by a lowering of ileal pH.

Keywords

Dietary lactoseLactuloseMagnesiumRat
Type
Effects of Lactose and Lactulose on Magnesium Absorption
Information
British Journal of Nutrition , Volume 70 , Issue 3 , November 1993 , pp. 747 - 756
Copyright
Copyright © The Nutrition Society 1993

References

Ammann, P., Rizzoli, R. & Fleisch, H. (1988). Influence of the disaccharide lactitol on intestinal absorption and body retention of calcium in rats. Journal of Nutrition 118, 793–795.CrossRefGoogle ScholarPubMed
Andrieux, C., Gadelle, D., Leprince, C. & Sacquet, E. (1989). Effects of some poorly digestible carbohydrates on bile acid bacterial transformations in the rat. British Journal of Nutrition 62, 103–119.CrossRefGoogle ScholarPubMed
Andrieux, C. & Sacquet, E. (1983). Effects of microflora and lactose on the absorption of calcium, phosphorus and magnesium in the hindgut of the rat. Réproduction, Nutrition Développement 23, 258–271.Google ScholarPubMed
Armbrecht, H. J. & Wasserman, R. H. (1976). Enhancement of Ca++ uptake by lactose in the rat small intestine. Journal of Nutrition 106, 1265–1271.CrossRefGoogle ScholarPubMed
Behling, A. R. & Greger, J. L. (1990). Importance of lactose in yoghurt for mineral utilization. Journal of Agriculture and Food Chemistry 38, 200–204.CrossRefGoogle Scholar
Beynen, A. C. (1989). Increased concentration of liver cholesterol in rats fed lactulose. Die Nahrung 33, 89–90.CrossRefGoogle Scholar
Beynen, A. C., West, C. E., Spaaij, C. J. K., Huisman, J., Van Leeuwen, P., Schutte, J. B. & Hackeng, W. H. L. (1990). Cholesterol metabolism, digestion rates and postprandial changes in serum of swine fed purified diets containing either casein or soybean protein. Journal of Nutrition 120, 422–430.CrossRefGoogle ScholarPubMed
Brink, E. J., Beynen, A. C., Dekker, P. R., Van Beresteijn, E. C. H. & Van der Meer, R. (1992). Interaction of calcium and phosphate decreases ileal magnesium solubility and apparent magnesium absorption in rats. Journal of Nutrition 122, 580–586.CrossRefGoogle ScholarPubMed
Brink, E. J., Dekker, P. R., Van Beresteijn, E. C. H. & Beynen, A. C. (1991). Inhibitory effect of dietary soybean protein vs. casein on magnesium absorption in rats. Journal of Nutrition 121, 1374–1381.CrossRefGoogle ScholarPubMed
Bronner, F. (1987). Intestinal calcium absorption, mechanisms and applications. Journal of Nutrition 117, 1347–1352.CrossRefGoogle ScholarPubMed
Debiec, H. & Lorenc, R. (1988). Influence of lactose on phosphate metabolism in rats. British Journal of Nutrition 59, 87–92.CrossRefGoogle ScholarPubMed
De Groot, A. P. & Hoogendoorn, P. (1957). The detrimental effect of lactose. 11. Quantitative lactase determinations in various mammals. Netherlands Milk and Dairy Journal 11, 29C303.Google Scholar
Favus, M. J. & Angeid-Backmann, E. (1984). Effects of lactose on calcium absorption and secretion by rat ileum. American Journal of Physiology 246, G281–LG285.Google ScholarPubMed
Fournier, P., Dupuis, Y. & Fournier, A. (1971). Effect of lactose on the absorption of alkaline earth metals and intestinal lactase activity. Israel Journal of Medical Sciences 7, 389–391.Google ScholarPubMed
Greenwald, I., Redish, J. & Kibrick, A. C. (1940). The dissociation of calcium and magnesium phosphates. Journal of Biological Chemistry 135, 65–76.Google Scholar
Greger, J. L., Gutkowski, C. M. & Khazen, R. R. (1989). Interactions of lactose with calcium, magnesium and zinc in rats. Journal of Nutrition 119, 1691–1697.CrossRefGoogle ScholarPubMed
Greger, J. L., Krzykowski, C. E., Khazen, R. R. & Krashoc, C. (1987). Mineral utilization by rats fed commercially available calcium supplements or milk. Journal of Nutrition 117, 717–724.CrossRefGoogle ScholarPubMed
Hardwick, L. L., Jones, M. R., Brautbar, N. & Lee, D. B. N. (1991). Magnesium absorption: mechanisms and the influence of vitamin D, calcium and phosphate. Journal of Nutrition 121, 13–23.CrossRefGoogle ScholarPubMed
Henskens, Y. M. C., Ritskes-Hoitinga, J., Mathot, J. N. J. J., Van Camp, I. & Beynen, A. C. (1991). The influence of dietary lactose on phosphorus-induced nephrocalcinosis in female rats. International Journal for Vitamin and Nutrition Research 61, 77–86.Google ScholarPubMed
Hoek, A. C., Lemmens, A. G., Mullink. J. W. M. A. & Beynen, A. C. (1988). Influence of dietary calcium:phosphorus ratio on mineral excretion and nephrocalcinosis in female rats. Journal of Nutrition 118, 1210–1216.CrossRefGoogle ScholarPubMed
Leichter, J. & Tolensky, A. F. (1975). Effect of dietary lactose on the absorption of protein, fat and calcium in the postweanling rat. American Journal of Clinical Nutrition 28, 238–241.CrossRefGoogle Scholar
Mars, Y. W. H. M., Lemmens, A. G. & Beynen, A. C. (1988). Dietary phosphorus and nephrocalcinosis in female rats. Nutrition Reports International 38, 249–258.Google Scholar
Petith, M. M. & Schedl, H. P. (1976). Intestinal adaptation to dietary calcium restriction: in vivo cecal and colonic calcium transport in the rat. Gastroenterology 71, 1039–1042.Google ScholarPubMed
Pomare, E. W., Branch, W. J. & Cummings, J. H. (1985). Carbohydrate fermentation in the human colon and its relation to acetate concentrations in venous blood. Journal of Clinical Investigation 75, 1448–1454.CrossRefGoogle ScholarPubMed
Recker, R. R., Bammi, A., Barger-Lux, J. & Heaney, R. (1988). Calcium absorbability from milk products, an imitation milk and calcium carbonate. American Journal of Clinical Nutrition 47, 93–95.CrossRefGoogle ScholarPubMed
Schaafsma, G., Dekker, P. R. & De Waard, H. (1988). Nutritional aspects of yoghurt. 2. Bioavailability of essential minerals and trace elements. Netherlands Milk and Dairy Journal 42, 135–146.Google Scholar
Schutte, J. B., De Jong, J., Van Weerden, E. J. & Tamminga, S. (1992). Nutritional implications of L-arabinose in pigs. British Journal of Nutrition 68, 195–207.CrossRefGoogle ScholarPubMed
Sheikh, M. S., Santa Ana, C. A., Nicar, M. J., Schiller, L. R. & Fordtran, J. S. (1987). Gastrointestinal absorption of calcium salts. New England Journal of Medicine 317, 532–536.CrossRefGoogle ScholarPubMed
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