Skip to main content Accessibility help
×
Home

Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids

  • I. Wąsowska (a1) (a2), M. R. G. Maia (a1) (a3), K. M. Niedźwiedzka (a1) (a2), M. Czauderna (a2), J. M. C. Ramalho Ribeiro (a3), E. Devillard (a1), K. J. Shingfield (a4) and R. J. Wallace (a1)...

Abstract

Dietary cis-9, trans-11-conjugated linoleic acid (CLA) is generally thought to be beneficial for human health. Fish oil added to ruminant diets increases the CLA concentration of milk and meat, an increase thought to arise from alterations in ruminal biohydrogenation of unsaturated fatty acids. To investigate the mechanism for this effect, in vitro incubations were carried out with ruminal digesta and the main biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens. Linoleic acid (LA) or α-linolenic acid (LNA) was incubated (1·67g/l) with strained ruminal digesta from sheep receiving a 50:50 grass hay–concentrate ration. Adding fish oil (up to 4·17g/l) tended to decrease the initial rate of LA (P=0·025) and LNA (P=0·137) disappearance, decreased (P<0·05) the transient accumulation of conjugated isomers of both fatty acids, and increased (P<0·05) the accumulation of trans-11-18:1. Concentrations of EPA (20:5 n-3) or DHA (22:6 n-3), the major fatty acids in fish oil, were low (100mg/l or less) after incubation of fish oil with ruminal digesta. Addition of EPA or DHA (50mg/l) to pure cultures inhibited the growth and isomerase activity of B. fibrisolvens, while fish oil had no effect. In contrast, similar concentrations of EPA and DHA had no effect on biohydrogenation of LA by mixed digesta, while the addition of LA prevented metabolism of EPA and DHA. Neither EPA nor DHA was metabolised by B. fibrisolvens in pure culture. Thus, fish oil inhibits ruminal biohydrogenation by a mechanism which can be interpreted partly, but not entirely, in terms of its effects on B. fibrisolvens.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr R. John Wallace, fax +44 1224 716687, email rjw@rowett.ac.uk

References

Hide All
AbuGhazaleh, AA & Jenkins, TCDisappearance of docosahexaenoic and eicosapentaenoic acids from cultures of mixed ruminal microorganisms. J Dairy Sci 2004a 87, 645651.
AbuGhazaleh, AA & Jenkins, TCShort communication: Docosahexaenoic acid promotes vaccenic acid accumulation in mixed ruminal cultures when incubated with linoleic acid. J Dairy Sci 2004b 87, 10471050..
Beam, TM, Jenkins, TC, Moate, PJ, Kohn, RA, Palmquist, DLEffects of amount and source of fat on the rates of lipolysis and biohydrogenation of fatty acids in ruminal contents. J Dairy Sci 2000 83, 25642573.
Chaudhary, LC, McKain, N, Richardson, AJ, Barbier, M, Charbonnier, J & Wallace, RJScreening for Fusocillus: factors that affect the detection of ruminal bacteria which form stearic acid from linoleic acid. Repr Nutr Develop 44 Suppl. 2004 1, S65.
Chow, TT, Fievez, V, Moloney, AP, Raes, K, Demeyer, D & De Smet, SEffect of fish oil on in vitro rumen lipolysis, apparent biohydrogenation of linoleic and linolenic acids and accumulation of biohydrogenation intermediates. Anim Feed Sci Technol 2004 117, 112.
Christie, WWGas chromatography-mass spectrometry methods for structural analysis of fatty acids. Lipids 1998 33, 343353.
Christie, WWLipid analysis. Isolation, separation, identifi-cation and structural analysis of lipids. Bridgwater, UK:. The Oily Press. 2003.
Dohme, F, Fievez, V, Raes, K & Demeyer, DIIncreasing levels of two different fish oils lower ruminal biohydrogenation of eicosapentaenoic and docosahexaenoic acid in vitro. Anim Res 2003 52, 309320.
Donovan, DC, Schingoethe, DJ, Baer, RJ, Ryali, J, Hippen, AR & Franklin, STInfluence of dietary fish oil on conjugated linoleic acid and other fatty acids in milk fat from lactating dairy cows. J Dairy Sci 2000 83, 26202628.
Fay, L, Richli, ULocation of double bonds in polyunsaturated fatty acids by gas chromatography-mass spectrometry after 4,4-dimethyloxazoline derivatization. J Chromatog 1991 541, 8998.
Fellner, V, Sauer, FD & Kramer, JKSteady-state rates of linoleic acid biohydrogenation by ruminal bacteria in continuous culture. J Dairy Sci 1995 78, 18151823.
Folch, J, Lees, M & Sloane-Stanley, GHA simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957 226, 497509.
Fujimoto, K, Kimoto, H, Shishikura, M, Endo, Y & Ogimoto, KBiohydrogenation of linoleic acid by anaerobic bacteria isolated from the rumen. Biosci Biotech Biochem 1993 57, 10261027.
Givens, DI & Shingfield, KJFood derived from animals: the impact of animal nutrition on their nutritive value and ability to sustain long-term health. Nutr Bull 2004 29, 325332.
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KV & Bauman, DEonjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta(9)-desaturase. C. J NutrC. J Nutr 2000 130, 22852291.
Gulati, SK, Ashes, JR, Scott, TWHydrogenation of eicosapentaenoic and decahexaenoic acids and their incorporation into milk fat. Anim Feed Sci Technol 1999 79, 5764.
Harfoot, CGLipid metabolism in the rumen. In Lipid Metabolism in Ruminant Animals, pp (Christie, WWOxford:. Pergamon Press. 1981. 2155.
Harfoot, CG, Crouchman, ML, Noble, RC & Moore, JHCompetition between food particles and rumen bacteria in the uptake of long-chain fatty acids and triglycerides. J Appl Bacteriol 1974 37, 633641.
Harfoot, CG & Hazlewood, GPLipid metabolism in the rumen. In The Rumen Microbial Ecosystem, pp (Hobson, PN & CS, Stewart) London:. Chapman & Hall. 1997. 382426.
Herbert, D, Phipps, PJ & Strange, REChemical analysis of microbial cells. In Methods in Microbiology, vol 5B. pp (Norris, JR & Ribbons, DW) London:. Academic Press. 1971. 209344.
Hobson, PNRumen bacteria. In Methods in Microbiology, vol.3B. (Norris, JR & Ribbons, DW) London:. Academic Press 1969. 133149.
Kaluzny, MA, Duncan, LA, Merritt, MV & Epps, DERapid separation of lipid classes in high yield and purity using bonded phase columns. J Lipid Res 1985 26, 135140.
Kepler, CR, Tove, SBBiohydrogenation of unsaturated fatty acids. 3. Purification and properties of a linoleate delta-12-cis,- delta-11-trans-isomerase fromButyrivibrio fibrisolvens. J Biol Chem 1967 242, 56865692.
Kepler, CR, Tucker, WP & Tove, SBBiohydrogenation of unsaturated fatty acids. IV. Substrate specificity and inhibition of linoleate delta-12-cis, delta-11-trans-isomerase from Butyrivibrio fibrisolvens. J Biol Chem 1970 245, 36123620.
Kritchevsky, DAntimutagenic and some other effects of conjugated linoleic acid. Br J Nutr 2000 83, 459465.
Lawson, RE, Moss, AR, Givens, DIThe role of dairy products in supplying conjugated linoleic acid to man's diet: a review. Nutr Res Rev 2001 14, 153172.
Lee, MRF, Tweed, JKS, Moloney, AP & Scollan, NDThe effects of fish oil supplementation on rumen metabolism and the biohydrogenation of unsaturated fatty acids in beef steers given diets containing sunflower oil. Anim Sci 2005 80, 361367.
Legay-Carmier, F, Bauchart, D & Doreau, MDistribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soyabean oil. Br J Nutr 1989 61, 725740.
Loor, JJ, Ueda, K, Ferlay, A, Chilliard, Y & Doreau, MIntestinal flow and digestibility of trans fatty acids and conjugated linoleic acids (CLA) in dairy cows fed a high-concentrate diet supplemented with fish oil, linseed oil, or sunflower oil. Anim Feed Sci Technol 2005 119, 203225.
McKain, N, Chaudhary, LC, Walker, ND, Pizette, F, Koppova, I, McEwan, NR, Kopecny, J, Vercoe, PE & Wallace, RJRelation between phylogenetic position and fatty acid metabolism of different Butyrivibrio isolates from the rumen. Repr Nutr Develop 44, Suppl 2004 1, S64.
Martin, SA & Jenkins, TCFactors affecting conjugated linoleic acid and trans-C18:1 fatty acid production by mixed ruminal bacteria. J Anim Sci 2002 80, 33473352.
Noble, RC, Noble, JH, Harfoot, CGObservations on the pattern of biohydrogenation of esterified and unesterified linoleic acid in the rumen. Br J Nutr 1974 31, 99108.
Offer, NW, Marsden, M, Dixon, J, Speake, BK & Thacker, FEEffect of dietary fat supplements on levels of n-3 poly-unsaturated fatty acids, trans acids and conjugated linoleic acid in bovine milk. Anim Sci 1999 69, 613625.
Parodi, PWConjugated linoleic acid and other anticarcinogenic agents of bovine milk fat. J Dairy Sci 1999 82, 13391349.
Polan, CE & McNeill, JJ, Tove, SBBiohydrogenation of unsaturated fatty acids by rumen bacteria. J Bacteriol 1964 88, 10561064.
Scollan, ND, Choi, NJ, Kurt, E, Fisher, AV, Enser, M & Wood, JDManipulating the fatty acid composition of muscle and adipose tissue in beef cattle. Br J Nutr 2001a 85, 115124.
Scollan, ND, Dhanoa, MS, Choi, NJ, Maeng, WJ, Enser, M & Wood, JDBiohydrogenation and digestion of long chain fatty acids in steers fed on different sources of lipid. J Agric Sci (Camb) 2001b 136, 345355.
Shingfield, KJ, Ahvenjarvi, S, Toivonen, V, Arola, A, Nurmela, KVV, Huhtanen, P & Griinari, JMEffect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Anim Sci 2003 77, 165179.
Stewart, CS, Flint, HJ & Bryant, MPThe rumen bacteria. In The Rumen Microbial Ecosystem, pp (Hobson, PN & Stewart, CS) London:. Chapman & Hall. 1997. 1072.
Troegeler-Meynadier, A, Nicot, MC, Bayourthe, C, Moncoulon, R & Enjalbert, FEffects of pH and concentrations of linoleic and linolenic acids on extent and intermediates of ruminal biohydrogenation in vitro. J Dairy Sci 2003 86, 40544063.
van, de, Vossenberg, JL & Joblin, KNBiohydrogenation of C18 unsaturated fatty acids to stearic acid by a strain of Butyrivibrio hungatei from the bovine rumen. Lett Appl Microbiol 2003 37, 424428.
Wachira, AM, Sinclair, LA, Wilkinson, RG, Enser, M, Wood, JD & Fisher, AVEffects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. Br J Nutr 2002 88, 697709.
Wachira, AM, Sinclair, LA, Wilkinson, RG, Hallett, K, Enser, M & Wood, JDRumen biohydrogenation of n-3 polyunsaturated fatty acids and their effects on microbial efficiency and nutrient digestibility in sheep. J Agric Sci (Camb) 135 2000, 419428.
Wallace, RJ, Brammall, MLThe role of different species of rumen bacteria in the hydrolysis of protein in the rumen. J Gen Microbiol 1985 131, 821832.
Wąsowska, I, Maia, M, Czauderna, M, Ramalho-Ribeiro, JMC & Wallace, RJInhibition of ruminal biohydrogenation of linoleic acid by fish oil. Reprod Nutr Develop 44, Suppl 2004 1, S64S65.
Wilde, PF & Dawson, RMCThe biohydrogenation of a-linolenic and oleic acid by rumen micro-organisms. Biochem J 1966 98, 469475.
Williams, CMDietary fatty acids and human health. Ann Zootech 2000 49, 165180.
Wonsil, BJ, Herbein, JH & Watkins, BADietary and ruminally derived trans-18:1 fatty acids alter bovine milk lipids. J Nutr 1994 124, 556565.
Zoetendal, EG, Collier, CT, Koike, S, Mackie, RI & Gaskins, HRMolecular ecological analysis of the gastrointestinal microbiota: a review. J Nutr 2004 134, 465472.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed