Skip to main content
×
Home
    • Aa
    • Aa

Modulation of the faecal microbiome of healthy adult dogs by inclusion of potato fibre in the diet

  • Matthew R. Panasevich (a1), Katherine R. Kerr (a1), Ryan N. Dilger (a1), George C. Fahey (a1), Laetitia Guérin-Deremaux (a2), Gary L. Lynch (a3), Daniel Wils (a2), Jan S. Suchodolski (a4), Jörg M Steer (a4), Scot E. Dowd (a5) and Kelly S. Swanson (a1)...
Abstract

Inclusion of fermentable fibres in the diet can have an impact on the hindgut microbiome and provide numerous health benefits to the host. Potato fibre (PF), a co-product of potato starch isolation, has a favourable chemical composition of pectins, resistant and digestible starch, cellulose, and hemicelluloses. The objective of the present study was to evaluate the effect of increasing dietary PF concentrations on the faecal microbiome of healthy adult dogs. Fresh faecal samples were collected from ten female dogs with hound bloodlines (6·13 (sem 0·17) years; 22·0 (sem 2·1) kg) fed five test diets containing graded concentrations of PF (0, 1·5, 3, 4·5 or 6 % as-fed; Roquette Frères) in a replicated 5 × 5 Latin square design. Extraction of DNA was followed by amplification of the V4–V6 variable region of the 16S rRNA gene using barcoded primers. Sequences were classified into taxonomic levels using Basic Local Alignment Search Tool (BLASTn) against a curated GreenGenes database. Inclusion of PF increased (P< 0·05) the faecal proportions of Firmicutes, while those of Fusobacteria decreased (P< 0·05). Similar shifts were observed at the genus level and were confirmed by quantitative PCR (qPCR) analysis. With increasing concentrations of PF, faecal proportions of Faecalibacterium increased (P< 0·05). Post hoc Pearson's correlation analysis showed positive (P< 0·05) correlations with Bifidobacterium spp. and butyrate production and Lactobacillus spp. concentrations. Overall, increases in the proportion of Faecalibacterium (not Lactobacillus/Bifidobacterium, as confirmed by qPCR analysis) and faecal SCFA concentrations with increasing dietary PF concentrations suggest that PF is a possible prebiotic fibre.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Modulation of the faecal microbiome of healthy adult dogs by inclusion of potato fibre in the diet
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Modulation of the faecal microbiome of healthy adult dogs by inclusion of potato fibre in the diet
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Modulation of the faecal microbiome of healthy adult dogs by inclusion of potato fibre in the diet
      Available formats
      ×
Copyright
Corresponding author
* Corresponding author: Dr K. S. Swanson, fax +1 217 333 7861, email ksswanso@illinois.edu
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

1 ZA Khachatryan , ZA Ktsoyan , GP Manukyan , et al. (2008) Predominant role of host genetics in controlling the composition of gut microbiota. PLoS ONE 3, e3064.

2 D Mariat , O Firmesse , F Levenez , et al. (2009) The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age. BMC Microbiol 9, 123128.

4 S Hooda , BM Boler , MC Serao , et al. (2012) 454 Pyrosequencing reveals a shift in fecal microbiota of healthy adult men consuming polydextrose or soluble corn fiber. J Nutr 142, 12591265.

5 IS Middelbos , BM Vester Boler , A Qu , et al. (2010) Phylogenetic characterization of fecal microbial communities of dogs fed diets with or without supplemental dietary fiber using 454 pyrosequencing. PLoS ONE 5, e9768.

6 F Mayer (1998) Potato pulp: properties, physical modification and applications. Polym Degrad Stab 59, 231235.

8 M Olesen , E Gudmand-Hoyer , M Norsker , et al. (1998) Fermentability of an enzymatically modified solubilised potato polysaccharide (SPP). Eur J Clin Nutr 52, 110114.

10 MR Panasevich , MC Rossoni Serao , MRC de Godoy , et al. (2013) Potato fiber as a dietary fiber source in dog foods. J Anim Sci 91, 53445352.

16 RC Edgar (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26, 24602461.

17 KA Capone , SE Dowd , GN Stamatas , et al. (2011) Diversity of the human skin microbiome early in life. J Invest Dermatol 131, 20262032.

20 KS Swanson , SE Dowd , JS Suchodolski , et al. (2011) Phylogenetic and gene-centric metagenomics of the canine intestinal microbiome reveals similarities with humans and mice. ISME J 5, 639649.

22 G Rossi , G Pengo , M Caldin , et al. (2014) Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or probiotic VSL#3 strains in dogs with idiopathic inflammatory bowel disease. PLOS ONE 9, e94699.

23 E Malinen , T Rinttila , K Kajander , et al. (2005) Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. Am J Gastroenterol 100, 373382.

25 JM Wong , R de Souza , CW Kendall , et al. (2006) Colonic health: fermentation and short chain fatty acids. J Clin Gastroenterol 40, 235243.

27 MA Vinolo , HG Rodrigues , RT Nachbar , et al. (2011) Regulation of inflammation by short chain fatty acids. Nutrients 3, 858876.

30 S Macfarlane & GT Macfarlane (2003) Regulation of short-chain fatty acid production. Proc Nutr Soc 62, 6772.

31 HM Hamer , D Jonkers , K Venema , et al. (2008) Review Article: the role of butyrate on colonic function. Aliment Pharmacol Ther 27, 104119.

32 C Manichanh , L Rigottier-Gois , E Bonnaud , et al. (2006) Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach. Gut 55, 205211.

33 H Sokol , B Pigneur , L Watterlot , et al. (2008) Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proc Natl Acad Sci U S A 105, 1673116736.

34 C Ramirez-Farias , K Slezak , Z Fuller , et al. (2009) Effect of inulin on the human gut microbiota: stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii. Br J Nutr 101, 541550.

36 A Swidsinski , Y Dorffel , V Loening-Baucke , et al. (2011) Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum. Gut 60, 3440.

37 KW Bennett & A Eley (1993) Fusobacteria: new taxonomy and related diseases. J Med Microbiol 39, 246254.

39 V Tremaroli & F Backhed (2012) Functional interactions between the gut microbiota and host metabolism. Nature 489, 242249.

40 E Olano-Martin , GR Gibson & RA Rastell (2002) Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides. J Appl Microbiol 93, 505511.

41Jumpstart Consortium Human Microbiome Project Data Generation Working Group (2012) Evaluation of 16S rDNA-based community profiling for human microbiome research. PLOS ONE 7, e39315.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

British Journal of Nutrition
  • ISSN: 0007-1145
  • EISSN: 1475-2662
  • URL: /core/journals/british-journal-of-nutrition
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Type Description Title
WORD
Supplementary Materials

Panasevich Supplementary Material
Table S1 and Figures S1-S2

 Word (154 KB)
154 KB

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 29
Total number of PDF views: 327 *
Loading metrics...

Abstract views

Total abstract views: 560 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 25th September 2017. This data will be updated every 24 hours.