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The nutraceutical role of the Phaseolus vulgaris α-amylase inhibitor

  • Wokadala Cuthbert Obiro (a1), Tao Zhang (a1) and Bo Jiang (a1)
Abstract

The present review assesses the potential of the Phaseolus vulgaris α-amylase inhibitor isoform 1 (α-AI1) starch blockers as a widely used remedy against obesity and diabetes. Consumption of the α-amylase inhibitor causes marginal intraluminal α-amylase activity facilitated by the inhibitor's appropriate structural, physico-chemical and functional properties. As a result there is decreased postprandial plasma hyperglycaemia and insulin levels, increased resistance of starch to digestion and increased activity of colorectal bacteria. The efficacy and safety of the amylase inhibitor extracts, however, depend on the processing and extraction techniques used. The extracts are potential ingredients in foods for increased carbohydrate tolerance in diabetics, decreased energy intake for reducing obesity and for increased resistant starch. Research developments in the distribution and biosynthesis of the α-amylase inhibitor, relevant physico-chemical properties, the molecular starch-blocking mechanism, anti-obesity and anti-diabetes effects, safety of extracts and the need for research into their potential anti-colorectal cancer effect are discussed.

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Corresponding author
*Corresponding author: Dr Bo Jiang, fax +86 510 85809610, email bjiang@sytu.edu.cn
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1 WJ Broughton , G Hernández , M Blair , S Beebe , P Gepts & J Vanderleyden (2003) Beans (Phaseolus spp.) – model food legumes. Plant Soil 252, 55128.

3 DE Bowman (1945) Amylase inhibitor of navy bean. Science 102, 358359.

5 D Chokshi (2006) Toxicity studies of blockal, a dietary supplement containing Phase 2 starch neutralizer (Phase 2), a standardized extract of the common white kidney bean (Phaseolus vulgaris). Int J Toxicol 25, 361371.

6 P Layer , GL Carlson & EP DiMagno (1985) Partially purified white bean amylase inhibitor reduces starch digestion in vitro and inactivates intraduodenal amylase in humans. Gastroenterology 88, 18951902.

7 P Layer , AR Zinsmeister & EP DiMagno (1986) Effects of decreasing intraluminal amylase activity on starch digestion and postprandial gastrointestinal function in humans. Gastroenterology 91, 4148.

10 BM Popkin & CM Doak (1998) The obesity epidemic is a worldwide phenomenon. Nutr Rev 56, 106114.

12 PT James , R Leach , E Kalamara & M Shayeghi (2001) The worldwide obesity epidemic. Obes Res 9, 22882338.

13 AH Mokdad , ES Ford , BA Bowman , WH Dietz , F Vinicor , VS Bales & JS Marks (2003) Prevalence of obesity, diabetes, and obesity-related health risk factors, 2001. JAMA 289, 7679.

17 M Kusaba-Nakayama , M Ki , M Iwamoto , R Shibata , M Sato & K Imaizumi (2000) CM3, one of the wheat α-amylase inhibitor subunits, and binding of IgE in sera from Japanese with atopic dermatitis related to wheat. Food Chem Toxicol 38, 179185.

18 R Sanchez-Monge , G Garcia-Casado , C Lopez-Otin , A Armentia & G Salcedo (1997) Wheat flour peroxidase is a prominent allergen associated with baker's asthma. Clin Exp Allergy 27, 11301137.

19 G Garcia-Casado , A Armentia , R Sanchez-Monge , JM Malpica & G Salcedo (1996) Rye flour allergens associated with baker's asthma. Correlation between in vivo and in vitro activities and comparison with their wheat and barley homologues. Clin Exp Allergy 26, 428435.

20 OL Franco , DJ Rigden , FR Melo & MF Grossi-de-Sa (2002) Plant α-amylase inhibitors and their interaction with insect α-amylases; structure, function and potential for crop protection. FEBS J 269, 397412.

21 AM Iguti & FM Lajolo (1991) Occurrence and purification of α-amylase isoinhibitors in bean (Phaseolus vulgaris L.) varieties. J Agric Food Chem 39, 21312136.

22 M Ishimoto , K Suzuki , M Iwanaga , F Kikuchi & K Kitamura (1995) Variation of seed α-amylase inhibitors in the common bean. Theor Appl Genet 90, 425429.

23 M Ishimoto & K Kitamura (1991) Effect of absence of seed α-amylase inhibitor on the growth inhibitory activity to azuki bean weevil (Callosobruchus chinensis) in common bean (Phaseolus vulgaris L.). Jpn J Breed 41, 231240.

24 K Suzuki , M Ishimoto , F Kikuchi & K Kitamura (1993) Growth inhibitory effect of an α-amylase inhibitor from the wild common bean resistant to the Mexican bean weevil (Zabrotes subfasciatus). Jpn J Breed 43, 257265.

25 J Moreno , T Altabella & MJ Chrispeels (1990) Characterization of α-amylase inhibitor, a lectin-like protein in the seeds of Phaseolus vulgaris L. Plant Physiol 92, 703709.

26 J Moreno & MJ Chrispeels (1989) A lectin gene encodes the α-amylase inhibitor of the common bean. Proc Natl Acad Sci U S A 86, 78857889.

27 JJ Pueyo , DC Hunt & MJ Chrispeels (1993) Activation of bean (Phaseolus vulgaris) α-amylase inhibitor requires proteolytic processing of the proprotein. Plant Physiol 101, 13411348.

28 NM Young & RP Oomen (1992) Analysis of sequence variation among legume lectins. A ring of hypervariable residues forms the perimeter of the carbohydrate-binding site. J Mol Biol 228, 924934.

29 SC Lee , PL Gepts & JR Whitaker (2002) Protein structures of common bean (Phaseolus vulgaris) α-amylase inhibitors. J Agric Food Chem 50, 66186627.

30 F Finardi-Filho , TE Mirkov & MJ Chrispeels (1996) A putative precursor protein in the evolution of the bean α-amylase inhibitor. Phytochemistry 43, 5762.

33 F Sparvoli , C Lanave , A Santucci , R Bollini & L Lioi (2001) Lectin and lectin-related proteins in lima bean (Phaseolus lunatus L.) seeds: biochemical and evolutionary studies. Plant Mol Biol 45, 587597.

34 M Ishimoto , T Yamada & A Kaga (1999) Insecticidal activity of an α-amylase inhibitor-like protein resembling a putative precursor of α-amylase inhibitor in the common bean, Phaseolus vulgaris L. Biochim Biophys Acta 1432, 104112.

35 V Le Berre-Anton , V Nahoum , F Payan & P Rouge (2000) Molecular basis for the specific binding of different α-amylase inhibitors from Phaseolus vulgaris seeds to the active site of α-amylase. Plant Physiol Biochem 38, 657665.

36 A Santino , MG Daminati , A Vitale & R Bollini (1992) The α-amylase inhibitor of bean seed: two step proteolytic maturation in the protein storage vacuoles of the developing cotyledon. Physiol Plant 85, 425432.

37 NM Young , P Thibault , DC Watson & MJ Chrispeels (1999) Post-translational processing of two α-amylase inhibitors and an arcelin from the common bean, Phaseolus vulgaris. FEBS Lett 446, 203206.

38 M Santimone , R Koukiekolo , Y Moreau , V Le Berre , P Rouge , G Marchis-Mouren & V Desseaux (2004) Porcine pancreatic α-amylase inhibition by the kidney bean (Phaseolus vulgaris) inhibitor (α-AI1) and structural changes in the α-amylase inhibitor complex. Biochim Biophys Acta 1696, 181190.

39 H Higaki & H Yamaguchi (1994) Reconstitution of Phaseolus vulgaris α-amylase inhibitor from isolated subunits. Biosci Biotechnol Biochem 58, 58.

40 S Sawada , Y Takeda & M Tashiro (2002) Primary structures of α- and β-subunits of α-amylase inhibitors from seeds of three cultivars of Phaseolus beans. J Protein Chem 21, 917.

41 BF Gibbs & I Alli (1998) Characterization of a purified α-amylase inhibitor from white kidney beans (Phaseolus vulgaris). Food Res Int 31, 217225.

42 C Bompard-Gilles , P Rousseau , P Rouge & F Payan (1996) Substrate mimicry in the active center of a mammalian α-amylase: structural analysis of an enzyme-inhibitor complex. Structure 4, 14411452.

43 I Kluh , M Horn , J Hyblova , J Hubert , L Doleckova-Maresova , Z Voburka , I Kudlikova , F Kocourek & M Mares (2005) Inhibitory specificity and insecticidal selectivity of α-amylase inhibitor from Phaseolus vulgaris. Phytochemistry 66, 3139.

44 K Kasahara , K Hayashi , T Arakawa , JS Philo , J Wen , S Hara & H Yamaguchi (1996) Complete sequence, subunit structure and complexes with pancreatic α-amylase of an α-amylase inhibitor from Phaseolus vulgaris white kidney beans. J Biochem 120, 177183.

45 R Koukiekolo , V Desseaux , Y Moreau , G Marchis-Mouren & M Santimone (2001) Mechanism of porcine pancreatic α-amylase inhibition of amylose and maltopentaose hydrolysis by α-, β-and γ-cyclodextrins. Eur J Biochem 268, 841848.

46 R Koukiekolo , V Le Berre-Anton , V Desseaux , Y Moreau , P Rouge , G Marchis-Mouren & M Santimone (1999) Mechanism of porcine pancreatic α-amylase inhibition of amylose and maltopentaose hydrolysis by kidney bean (Phaseolus vulgaris) inhibitor and comparison with that by acarbose. Eur J Biochem 265, 2026.

47 SC Lee & JR Whitaker (2000) The molecular weight of α-amylase inhibitior from white bean cv 858B (Phaseolus vulgaris L.) is 56 kDa, not 20 kDa. J Food Biochem 24, 5567.

48 V Le Berre-Anton , C Bompard-Gilles , F Payan & P Rouge (1997) Characterization and functional properties of the α-amylase inhibitor (α-AI) from kidney bean (Phaseolus vulgaris) seeds. Biochim Biophys Acta 1343, 3140.

49 FM Lajolo & F Finardi Filho (1985) Partial characterization of the amylase inhibitor of black beans (Phaseolus vulgaris), variety Rico 23. J Agric Food Chem 33, 132138.

50 M Kotaru , H Yoshikawa , T Ikeuchi , K Saito , K Iwami & F Ibuki (1987) An α-amylase inhibitor from cranberry bean (Phaseolus vulgaris): its specificity in inhibition of mammalian pancreatic α-amylases and formation of a complex with the porcine enzyme. J Nutr Sci Vitaminol (Tokyo) 33, 359367.

51 JR Powers & JR Whitaker (1977) Effect of several experimental parameters on combination of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor with porcine pancreatic α-amylase. J Food Biochem 1, 239260.

52 A Valencia , AE Bustillo , GE Ossa & MJ Chrispeels (2000) α-Amylases of the coffee berry borer (Hypothenemus hampei) and their inhibition by two plant amylase inhibitors. Insect Biochem Mol Biol 30, 207213.

53 CL Collins , PJ Eason , FR Dunshea , TJV Higgins & RH King (2006) Starch but not protein digestibility is altered in pigs fed transgenic peas containing α-amylase inhibitor. J Sci Food Agric 86, 18941899.

54 F Payan (2004) Structural basis for the inhibition of mammalian and insect α-amylases by plant protein inhibitors. Biochim Biophys Acta 1696, 171180.

55 V Nahoum , G Roux , V Anton , P Rougé , A Puigserver , H Bischoff , B Henrissat & F Payan (2000) Crystal structures of human pancreatic α-amylase in complex with carbohydrate and proteinaceous inhibitors. Biochem J 346, 201208.

56 R Maurus , A Begum , HH Kuo , A Racaza , S Numao , C Andersen , JW Tams , J Vind , CM Overall & SG Withers (2005) Structural and mechanistic studies of chloride induced activation of human pancreatic α-amylase. Protein Sci 14, 743755.

57 MF Ho & JR Whitaker (1993) Subunit structures and essential amino acid residues of white kidney bean (Phaseolus vulgaris) α-amylase inhibitors. J Food Biochem 17, 3552.

58 TE Mirkov , SV Evans , J Wahlstrom , L Gomez , NM Young & MJ Chrispeels (1995) Location of the active site of the bean α-amylase inhibitor and involvement of a Trp, Arg, Tyr triad. Glycobiology 5, 4550.

59 T Takahashi , S Hiramoto , S Wato , T Nishimoto , Y Wada , K Nagai & H Yamaguchi (1999) Identification of essential amino acid residues of an α-amylase inhibitor from Phaseolus vulgaris white kidney beans. J Biochem 126, 838844.

60 MCM Da Silva , MFG De Sá , MJ Chrispeels , RC Togawa & G Neshich (2000) Analysis of structural and physico-chemical parameters involved in the specificity of binding between α-amylases and their inhibitors. Protein Eng Des Sel 13, 167177.

61 M Qian (1997) Structure of a pancreatic α-amylase bound to a substrate analogue at 2·03 A resolution. Protein Sci 6, 22852296.

62 ER Wilcox & JR Whitaker (1984) Some aspects of the mechanism of complexation of red kidney bean α-amylase inhibitor and α-amylase. Biochemistry 23, 17831791.

64 M Boivin , AR Zinsmeister , VL Go & EP DiMagno (1987) Effect of a purified amylase inhibitor on carbohydrate metabolism after a mixed meal in healthy humans. Mayo Clin Proc 62, 249255.

66 NK Jain , M Boivin , AR Zinsmeister & EP DiMagno (1991) The ileum and carbohydrate-mediated feedback regulation of postprandial pancreaticobiliary secretion in normal humans. Pancreas 6, 495505.

68 GL Carlson , BU Li , P Bass & WA Olsen (1983) A bean α-amylase inhibitor formulation (starch blocker) is ineffective in man. Science 219, 393.

69 GW Bo-Linn , CA Santa Ana , SG Morawski & JS Fordtran (1982) Starch blockers – their effect on calorie absorption from a high-starch meal. N Engl J Med 307, 14131416.

71 DC Kilpatrick , C Green & PL Yap (1983) Lectin content of slimming pills. BMJ (Clin Res Ed) 286, 305.

73 M Kotaru , K Iwami , HY Yeh & F Ibuki (1989) In vivo action of α-amylase inhibitor from cranberry bean (Phaseolus vulgaris) in rat small intestine. J Nutr Sci Vitaminol (Tokyo) 35, 579588.

74 H Yoshikawa , M Kotaru , C Tanaka , T Ikeuchi & M Kawabata (1999) Characterization of kintoki bean (Phaseolus vulgaris) α-amylase inhibitor: inhibitory activities against human salivary and porcine pancreatic α-amylases and activity changes by proteolytic digestion. J Nutr Sci Vitaminol (Tokyo) 45, 797802.

75 M Kotaru , K Iwami , HYU Yeh & F Ibuki (1991) Resistance of cranberry bean (Phaseolus vulgaris) α-amylase inhibitor to intraluminal digestion and its movement along rat gastrointestine: further investigation using a radioactive probe and specific antiserum. Food Chem 42, 2937.

77 KB Harikumar , AM Jesil , MC Sabu & R Kuttan (2005) A preliminary assessment of the acute and subchronic toxicity profile of Phase 2: an α-amylase inhibitor. Int J Toxicol 24, 95102.

78 T Itoh , N Kita , Y Kurokawa , M Kobayashi , F Horio & Y Furuichi (2004) Suppressive effect of a hot water extract of Adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats. Biosci Biotechnol Biochem 68, 24212426.

84 D Chokshi (2007) Subchronic oral toxicity of a standardized white kidney bean (Phaseolus vulgaris) extract in rats. Food Chem Toxicol 45, 3240.

92 M Higuchi , M Suga & K Iwai (1983) Participation of lectin in biological effects of raw winged bean seeds on rats. Agric Biol Chem 47, 18791886.

96 J Carmalt , K Rosel , T Burns & E Janzen (2003) Suspected white kidney bean (Phaseolus vulgaris) toxicity in horses and cattle. Aust Vet J 81, 674676.

100 L Lioi , F Sparvoli & R Bollini (1999) Variation and genomic polymorphism of lectin-related proteins in Lima bean (Phaseolus lunatus L.) seeds. Genet Resour Crop Evol 46, 175182.

101 C Burbano , M Muzquiz , G Ayet , C Cuadrado & MM Pedrosa (1999) Evaluation of antinutritional factors of selected varieties of Phaseolus vulgaris. J Sci Food Agric 79, 14681472.

102 VC Sgarbieri (1989) Nutritional values of cereal products, beans and starches. World Rev Nutr Diet 60, 132198.

105 SG Haralampu (2000) Resistant starch – a review of the physical properties and biological impact of RS3. Carbohydr Polymer 41, 285292.

106 AP Nugent (2005) Health properties of resistant starch. Nutr Bull 30, 2754.

108 RK Le Leu , IL Brown , Y Hu & GP Young (2003) Effect of resistant starch on genotoxin-induced apoptosis, colonic epithelium, and lumenal contents in rats. Carcinogenesis 24, 13471352.

114 PR Holt , E Atillasoy , J Lindenbaum , SB Ho , JR Lupton , D McMahon & SF Moss (1996) Effects of acarbose on fecal nutrients, colonic pH, and short-chain fatty acids and rectal proliferative indices. Metabolism 45, 11791187.

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