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Phytate levels in diverse rat tissues: influence of dietary phytate

  • F. Grases (a1), B. M. Simonet (a1), R. M. Prieto (a1) and J.G March (a1)


Phytate (inositol hexaphosphate; InsP6) was determined in rat tissues fed on diets with different phytate contents, using a GC–mass detection methodology that permitted the evaluation of the total amount of this substance present in such tissues. The highest InsP6 concentrations were found in brain (5·89×10-2 (SE 5·7×10-3) MG/G DM), WHEREAS THE CONCENTRATIONS DETECTED IN KIDNEYS, LIVER AND BONE WERE SIMILAR TO EACH OTHER (1·96×10-3 (se 0·20×10-3), 3·11×10-3 (se 0·24×10-3), 1·77×10-3 (se 0·17×10-3) mg/g DM respectively) and 10-fold less than those detected in brain. When rats were fed on a purified diet in which InsP6 was undetectable, the InsP6 levels of the organs mentioned earlier decreased dramatically (9·0×10-4, 3·8×10-5, 1·4×10-5 mg/g DM in brain, kidneys and liver respectively) and in some cases became undetectable (bone). The addition of InsP6 to this purified diet led to the increase of InsP6 levels in these tissues. This clearly demonstrated that the majority of the InsP6 found in organs and tissues has a dietary origin and is not a consequence of endogenous synthesis. Consequently, considering that InsP6 could be involved in some important biological roles, the value of any diet on supplying this substance is noteworthy.

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Corresponding author

*Corresponding author: Dr Felix Grases, fax +34 971 17 34 26, email


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Anonymous (1987) Phytic acid: new doors open for a chelator. Lancet 19, 664666.
Brearley, CA & Hanke, DE (1996) Metabolic evidence for the order of addition of individual phosphates esters in the myo-inositol moiety of inositol hexakisphosphate in the duckweed Spirodela polyrhiza L. Biochemical Journal 314, 227233.
Bunce, CM, French, PJ, Allen, P, Mountford, JC, Moor, B, Greaves, MF, Michell, RH & Brown, G (1993) Comparison of the levels of inositol metabolites in transformed haemopoietic cells and their normal counterparts. Biochemical Journal 289, 667673.
Conte, A, Pizá, P, García-Raja, A, Grases, F, Costa-Bauzá, A & Prieto, RM (1999) Test of urinary lithogen risk: usefulness in the evaluation of renal lithiasis treatment using crystallisation inhibitors (citrate and phytate). Archivos Españoles de Urologia 52, 9499.
Davidsson, L, Galan, P, Cherouvrier, F, Kastenmayer, P, Juillerat, MA, Hercberg, S & Hurrell, RF (1997) Bioavailability in infants of iron from infant cereals: effect of dephytinization. American Journal of Clinical Nutrition 65, 916920.
Efanov, AM, Zaitsev, SV & Berggren, P (1997) Inositol hexakisphosphate stimulates non-Ca2+-mediated exocytosis of insulin by activation of protein kinase C. Proceedings of the National Academy of Sciences, USA 94, 44354439.
French, PJ, Bunce, CM, Stephens, LR, Lord, JM, McConnell, FM, Brown, G, Creba, JA & Michell, RH (1991) Changes in the levels of inositol lipids and phosphates during the differentiation of HL60 promyelocytic cells towards neutrophils or monocytes. Philosophical Transanctions of the Royal Society of London Series B - Biological Science 245, 193201.
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497509.
Grases, F, García-Gonzalez, R, Torres, JJ & Llobera, A (1998) The effects of phytic acid on renal stone formation in rats. Scandinavian Journal of Urology and Nephrology 32, 262265.
Grases, F, March, JG, Prieto, RM, Simonet, BM, Costa-Bauzá, A, García-Raja, A & Conte, A (2000a) Urinary phytate in calcium oxalate stone-formers and healthy people. Dietary effects on phytate excretion. Scandinavian Journal of Urology and Nephrology 34, 162164.
Grases, F, Prieto, RM, Simonet, BM & March, JG (2000b) Phytate prevents tissue calcifications in female rats. BioFactors 11, 171177.
Grases, F, Simonet, BM, March, JG & Prieto, RM (2000c) Inositol hexakisphosphate in urine: the relationship between oral intake and urinary excretion. British Journal of Urology International 85, 138142.
Harland, BF & Oberleas, D (1987) Phytate in foods. World Review of Nutrition and Dietetics 52, 235259.
Hawkins, PT, Poyner, DR, Jackson, TR, Letcher, AJ, Lander, DA & Irvine, RF (1993) Inhibition of iron-catalysed hydroxyl radical formation by inositol polyphosphates: a possible physiological function for myo-inositol hexakisphosphate. Biochemical Journal 294, 929934.
Hurrell, RF, Juillerat, MA, Reddy, MB, Lynch, SR, Dassenko, SA & Cook, JD (1992) Soy protein, phytate, and iron absorption in humans. American Journal of Clinical Nutrition 56, 573578.
Irvine, RF (1995) Inositide evolution: What can it tell us about functions? Biochemical Society Transactions 23, 2735.
Jackson, TR, Hallam, TJ, Downes, CP & Hanley, MR (1987) Receptor coupled events in bradykinin action: rapid production of inositol phosphates and regulation of cytosolic free Ca2+ in a neural cell line. EMBO Journal 6, 4954.
Kelsay, JL (1987) Effects of fiber, phytic acid and oxalic acid in the diet on mineral bioavailability. American Journal of Gastroenterology 32, 983986.
Larsson, O, Barker, CJ, Sjöholm Å, A, Carlqvist, H, Michell, RH, Bertorello, A, Nilsson, T, Honkanen, RE, Mayr, GW, Zwiller, J & Berggren, P (1997) Inhibition of phosphatases and increased Ca2+ channel activity by inositol hexakisphosphate. Science 278, 471474.
Luttrell, BM (1993) The biological relevance of the binding of calcium ions by inositol phosphates. Journal of Biological Chemistry 268, 15211524.
Maga, JA (1982) Phytate: Its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. Journal of Agricultural and Food Chemistry 30, 19.
March, JG, Simonet, BM, Grases, F & Salvador, A (1998) Indirect determination of phytic acid in urine. Analitica Chimica Acta 367, 6368.
March, JG, Simonet, BM & Grases, F (2000) Determination of phytic acid by GC–MS. Application to biological samples. Journal of Chromatography B. (In the Press).
March, JG, Villacampa, AI & Grases, F (1995) Enzymatic photometric determination of phytic acid with phytase from aspergillus Ficuum. Analitica Chimica Acta 300, 269272.
Menniti, FS, Oliver, KG, Putney, JW & Shears, SB (1993) Inositol phosphates and cell signaling: new views of InsP6. Trends in Biological Science 18, 5356.
Ongusaha, PP, Hughes, PJ, Davey, J & Michell, RH (1998) Inositol hexakisphosphate in Schizosaccharomyces pombe: synthesis from Ins (1,4,5)P3 and osmotic regulation. Biochemical Journal 335, 671679.
O'Rourke, F, Matthews, E & Feinstein, MB (1996) Isolation of InsP4 and InsP6 binding proteins for human platelets: InsP4 promotes Ca2+ efflux from inside-out plasma membrane vesicles containing 104 kDa GAP1IP4BP protein. Biochemical Journal 315, 10271034.
Pittet, D, Schlegel, W, Lew, DP, Monod, A & Mayr, GW (1989) Mass changes in inositol tetrakis- and pentakisphosphate isomers induced by chemotactic peptide stimulation in HL-60 cells. Journal of Biological Chemistry 264, 1848918493.
Sandberg, AS, Brune, M, Carlsson, NG, Hallberg, L, Skoglund, E & Rossander-Hulthen, L (1999) Inositol phosphate with different numbers of phosphate groups influence iron absorption in humans. American Journal of Clinical Nutrition 70, 240246.
Sandstrom, B & Sandberg, AS (1992) Inhibitory effects of isolated inositol phosphates on zinc absorption in humans. Journal of Trace Elements and Electrolytes in Health and Disease 6, 99103.
Sasakawa, N, Sharif, M & Hanley, MR (1995) Metabolism and biological activities of inositol pentakisphosphate and inositol hexakisphosphate. Biochemical Pharmacology 50, 137146.
Stephens, LR & Irvine, RF (1990) Stepwise phosphorylation of myo-inositol leading to myo-inositol hexakisphosphate in Dictyostelium. Nature 346, 580583.
Szwergold, BS, Graham, RA & Brown, TR (1987) Observation of inositol pentakis-hexakis-phosphates in mammalian tissues by 31P NMR. Biochemical and Biophysical Research Communications 149, 874881.
Vallejo, M, Jackson, T, Lightman, S & Hanley, MR (1987) Occurrence and extracellular actions of inositol pentakis- and hexakisphosphate in mammalian brain. Nature 330, 656658.
York, JD, Odom, AR, Murphy, R, Ives, EB & Wente, SR (1999) A phospholiphase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. Science 285, 96100.



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