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Short-term docosapentaenoic acid (22 : 5n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats

  • Gunveen Kaur (a1), Denovan P. Begg (a2) (a3), Daniel Barr (a3), Manohar Garg (a4), David Cameron-Smith (a3) and Andrew J. Sinclair (a1) (a3)
  • DOI:
  • Published online: 04 August 2009

The metabolic fate of dietary n-3 docosapentaenoic acid (DPA) in mammals is currently unknown. The aim of the present study was to determine the extent of conversion of dietary DPA to DHA and EPA in rats. Four groups of male weanling Sprague–Dawley rats (aged 5 weeks) were given 50 mg of DPA, EPA, DHA or oleic acid, daily for 7 d by gavage. At the end of the treatment period, the tissues were analysed for concentrations of long-chain PUFA. DPA supplementation led to significant increases in DPA concentration in all tissues, with largest increase being in adipose (5-fold) and smallest increase being in brain (1·1-fold). DPA supplementation significantly increased the concentration of DHA in liver and the concentration of EPA in liver, heart and skeletal muscle, presumably by the process of retroconversion. EPA supplementation significantly increased the concentration of EPA and DPA in liver, heart and skeletal muscle and the DHA concentration in liver. DHA supplementation elevated the DHA levels in all tissues and EPA levels in the liver. Adipose was the main tissue site for accumulation of DPA, EPA and DHA. These data suggest that dietary DPA can be converted to DHA in the liver, in a short-term study, and that in addition it is partly retroconverted to EPA in liver, adipose, heart and skeletal muscle. Future studies should examine the physiological effect of DPA in tissues such as liver and heart.

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*Corresponding author: Professor Andrew J. Sinclair, fax +61 3 9251 7282, email
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1TN Raju (1999) The Nobel Chronicles: 1982: Sune Karl Bergstrom (b 1916); Bengt Ingemar Samuelsson (b 1934); John Robert Vane (b 1927). Lancet 354, 1914.

2M Arita , M Yoshida , S Hong , (2005) Resolvin E1, an endogenous lipid mediator derived from omega-3 eicosapentaenoic acid, protects against 2,4,6-trinitrobenzene sulfonic acid-induced colitis. Proc Natl Acad Sci U S A 102, 76717676.

4M Tsuji , SI Murota & I Morita (2003) Docosapentaenoic acid (22 : 5, n-3) suppressed tube-forming activity in endothelial cells induced by vascular endothelial growth factor. Prostaglandins Leukot Essent Fatty Acids 68, 337342.

5K Kitajka , LG Puskas , A Zvara , (2002) The role of n-3 polyunsaturated fatty acids in brain: modulation of rat brain gene expression by dietary n-3 fatty acids. Proc Natl Acad Sci U S A 99, 26192624.

6M Fujikawa , K Yamazaki , T Hamazaki , (1994) Effect of eicosapentaenoic acid ethyl ester on albuminuria in streptozotocin-induced diabetic rats. J Nutr Sci Vitaminol (Tokyo) 40, 4961.

7H Shimizu , K Ohtani , Y Tanaka , (1995) Long-term effect of eicosapentaenoic acid ethyl (EPA-E) on albuminuria of non-insulin dependent diabetic patients. Diabetes Res Clin Pract 28, 3540.

8JT Brenna , N Salem Jr, AJ Sinclair , (2009) alpha-Linolenic acid supplementation and conversion to n-3 long-chain polyunsaturated fatty acids in humans. Prostaglandins Leukot Essent Fatty Acids 80, 8591.

9AJ Sinclair , NM Attar-Bashi & D Li (2002) What is the role of alpha-linolenic acid for mammals? Lipids 37, 11131123.

10Z Fu & AJ Sinclair (2000) Increased alpha-linolenic acid intake increases tissue alpha-linolenic acid content and apparent oxidation with little effect on tissue docosahexaenoic acid in the guinea pig. Lipids 35, 395400.

12AJ Sinclair (1975) Incorporation of radioactive polyunsaturated fatty acids into liver and brain of developing rat. Lipids 10, 175184.

13A Pawar & DB Jump (2003) Unsaturated fatty acid regulation of peroxisome proliferator-activated receptor alpha activity in rat primary hepatocytes. J Biol Chem 278, 3593135939.

14C Benistant , F Achard , S Ben Slama , (1996) Docosapentaenoic acid (22 : 5, n-3): metabolism and effect on prostacyclin production in endothelial cells. Prostaglandins Leukot Essent Fatty Acids 55, 287292.

16NJ Mann , GE Warrick , K O'Dea , (1994) The effect of linoleic, arachidonic and eicosapentaenoic acid supplementation on prostacyclin production in rats. Lipids 29, 157162.

17MA Williams , J Tinoco , YT Yang , (1989) Feeding pure docosahexaenoate or arachidonate decreases plasma triacylglycerol secretion in rats. Lipids 24, 753758.

18AJ Sanigorski , AJ Sinclair & T Hamazaki (1996) Platelet and aorta arachidonic and eicosapentaenoic acid levels and in vitro eicosanoid production in rats fed high-fat diets. Lipids 31, 729735.

19YS Huang , J Mitchell , K Jenkins , (1984) Effect of dietary depletion and repletion of linoleic acid on renal fatty acid composition and urinary prostaglandin excretion. Prostaglandins Leukot Med 15, 223228.

20AJ Sinclair , K O'Dea , G Dunstan , (1987) Effects on plasma lipids and fatty acid composition of very low fat diets enriched with fish or kangaroo meat. Lipids 22, 523529.

21W Stoffel , Eker, H Assad , (1970) Enzymatic studies on the mechanism of the retroconversion of C22-polyenoic fatty acids to their C20-homologues. Hoppe Seylers Z Physiol Chem 351, 15451554.

23JK Reddy & T Hashimoto (2001) Peroxisomal beta-oxidation and peroxisome proliferator-activated receptor alpha: an adaptive metabolic system. Annu Rev Nutr 21, 193230.

24MD Rosenthal , MC Garcia , MR Jones , (1991) Retroconversion and delta 4 desaturation of docosatetraenoate (22 : 4(n-6)) and docosapentaenoate (22 : 5(n-3)) by human cells in culture. Biochim Biophys Acta 1083, 2936.

25CT Chen , Z Liu , M Ouellet , (2009) Rapid beta-oxidation of eicosapentaenoic acid in mouse brain: an in situ study. Prostaglandins Leukot Essent Fatty Acids 80, 157163.

26R Portolesi , BC Powell & RA Gibson (2007) Competition between 24 : 5n-3 and ALA for Delta 6 desaturase may limit the accumulation of DHA in HepG2 cell membranes. J Lipid Res 48, 15921598.

27GR Ward , YS Huang , HC Xing , (1999) Effects of gamma-linolenic acid and docosahexaenoic acid in formulae on brain fatty acid composition in artificially reared rats. Lipids 34, 10571063.

28J Nasrollahzadeh , F Siassi , M Doosti , (2008) The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression. Lipids Health Dis 7, 45.

29ML Garg , E Sebokova , AB Thomson , (1988) Delta 6-desaturase activity in liver microsomes of rats fed diets enriched with cholesterol and/or omega 3 fatty acids. Biochem J 249, 351356.

30HP Cho , M Nakamura & SD Clarke (1999) Cloning, expression, and fatty acid regulation of the human delta-5 desaturase. J Biol Chem 274, 3733537339.

31WE Lands , B Libelt , A Morris , (1992) Maintenance of lower proportions of (n-6) eicosanoid precursors in phospholipids of human plasma in response to added dietary (n-3) fatty acids. Biochim Biophys Acta 1180, 147162.

33S Akiba , T Murata , K Kitatani , (2000) Involvement of lipoxygenase pathway in docosapentaenoic acid-induced inhibition of platelet aggregation. Biol Pharm Bull 23, 12931297.

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