Skip to main content Accessibility help
×
Home

Vitamin K absorption and kinetics in human subjects after consumption of 13C-labelled phylloquinone from kale

  • Janet A. Novotny (a1), Anne C. Kurilich (a1), Steven J. Britz (a1), David J. Baer (a1) and Beverly A. Clevidence (a1)...

Abstract

The absorption and plasma disappearance of vitamin K were investigated by uniformly labelling phylloquinone in kale with carbon-13, and by feeding the kale to study subjects. Seven healthy volunteers ingested a single 400 g serving of kale with 30 g vegetable oil. The kale provided 156 nmol of phylloquinone. Serial plasma samples were collected and analysed for the appearance of 13C-phylloquinone by HPLC–MS. Six of the subjects showed significant amounts of labelled phylloquinone in plasma, though one subject's plasma was not consistently enriched above the detection limit, and this subject's baseline plasma phylloquinone level was the lowest in the group. After ingestion of the labelled kale, plasma 13C-phylloquinone concentration increased rapidly to a peak between 6 and 10 h, and then rapidly decreased. Average peak plasma concentration for the six subjects with detectable 13C-phylloquinone was 2·1 nmol/l. Plasma concentration–time data were analysed by compartmental modelling. Modelling results demonstrated a mean (n 6) bioavailability of phylloquinone from kale to be 4·7 %. Plasma and tissue half-times for phylloquinone were found to be 8·8 and 215 h, respectively.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Vitamin K absorption and kinetics in human subjects after consumption of 13C-labelled phylloquinone from kale
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      Vitamin K absorption and kinetics in human subjects after consumption of 13C-labelled phylloquinone from kale
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      Vitamin K absorption and kinetics in human subjects after consumption of 13C-labelled phylloquinone from kale
      Available formats
      ×

Copyright

Corresponding author

*Corresponding author: Dr Janet A. Novotny, fax +1 301 504 9098, email janet.novotny@ars.usda.gov

References

Hide All
1 Jones, KS, Bluck, LJ, Wang, LY, et al. (2008) A stable isotope method for the simultaneous measurement of vitamin K1 (phylloquinone) kinetics and absorption. Eur J Clin Nutr 62, 12731281.
2 Shearer, MJ, Barkhan, P & Webster, GR (1970) Absorption and excretion of an oral dose of tritiated vitamin K1 in man. Br J Haematol 18, 297308.
3 Booth, SL, Lichtenstein, AH & Dallal, GE (2002) Phylloquinone absorption from phylloquinone-fortified oil is greater than from a vegetable in younger and older men and women. J Nutr 132, 26092612.
4 Gijsbers, BL, Jie, KS & Vermeer, C (1996) Effect of food composition on vitamin K absorption in human volunteers. Br J Nutr 76, 223239.
5 Garber, AK, Binkley, NC, Krueger, DC, et al. (1999) Comparison of phylloquinone bioavailability from food sources or a supplement in human subjects. J Nutr 129, 12011203.
6 Kurilich, AC, Britz, SJ, Clevidence, BA, et al. (2003) Isotopic labeling and LC–APCI–MS quantification for investigating absorption of carotenoids and phylloquinone from kale (Brassica oleracea). J Agric Food Chem 51, 48774883.
7 US Department of Agriculture ARS (2009) USDA National Nutrient Database for Standard Reference, Release 22.
8 McDowell, MA, Fryar, CD, Hirsch, R, et al. (2005) Anthropometric reference data for children and adults: U.S. population, 1999–2002. Adv Data 7, 15.
9 Booth, SL, Tucker, KL, McKeown, NM, et al. (1997) Relationships between dietary intakes and fasting plasma concentrations of fat-soluble vitamins in humans. J Nutr 127, 587592.
10 Shearer, MJ, Mallinson, C & Webster, GR, et al. (1971) Studies in man with tritiated vitamin K1 administered intravenously. Clin Sci 40, 23P.
11 Shearer, MJ, Mallinson, CN, Webster, GR, et al. (1972) Clearance from plasma and excretion in urine, faeces and bile of an intravenous dose of tritiated vitamin K 1 in man. Br J Haematol 22, 579588.
12 Shearer, MJ, McBurney, A & Barkhan, P (1974) Studies on the absorption and metabolism of phylloquinone (vitamin K1) in man. Vitam Horm 32, 513542.
13 Olson, RE, Chao, J, Graham, D, et al. (2002) Total body phylloquinone and its turnover in human subjects at two levels of vitamin K intake. Br J Nutr 87, 543553.
14 Bjornsson, TD, Meffin, PJ, Swezey, SE, et al. (1979) Effects of clofibrate and warfarin alone and in combination on the disposition of vitamin K1. J Pharmacol Exp Ther 210, 322326.
15 Jones, KS, Bluck, LJ, Wang, LY, et al. (2009) The effect of different meals on the absorption of stable isotope-labelled phylloquinone. Br J Nutr 102, 11951202.
16 Dolnikowski, GG, Sun, Z, Grusak, MA, et al. (2002) HPLC and GC/MS determination of deuterated vitamin K (phylloquinone) in human serum after ingestion of deuterium-labeled broccoli. J Nutr Biochem 13, 168174.
17 Erkkila, AT, Lichtenstein, AH, Dolnikowski, GG, et al. (2004) Plasma transport of vitamin K in men using deuterium-labeled collard greens. Metabolism 53, 215221.
18 Booth, SL, O'Brien-Morse, ME, Dallal, GE, et al. (1999) Response of vitamin K status to different intakes and sources of phylloquinone-rich foods: comparison of younger and older adults. Am J Clin Nutr 70, 368377.
19 Schurgers, LJ & Vermeer, C (2000) Determination of phylloquinone and menaquinones in food. Effect of food matrix on circulating vitamin K concentrations. Haemostasis 30, 298307.

Keywords

Vitamin K absorption and kinetics in human subjects after consumption of 13C-labelled phylloquinone from kale

  • Janet A. Novotny (a1), Anne C. Kurilich (a1), Steven J. Britz (a1), David J. Baer (a1) and Beverly A. Clevidence (a1)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed