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Phylogenetic position of a Pacific Northwest North American endemic cyanolichen, Nephroma occultum (Ascomycota, Peltigerales)

Published online by Cambridge University Press:  22 August 2006

Department of Botany, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2
University of Northern British Columbia, Prince George, British Columbia, Canada, V2N 4Z9
Enlichened Consulting Ltd, Edgewood Blue, Box 131, Clearwater, BC, Canada, V0E 1N0
Department of Ecology and Evolutionary Biology, University of Connecticut, 75 N. Eagleville Road, U-43, Storrs, CT 06269-3043, USA


Nephroma occultum is a COSEWIC listed cyanolichen of “Special Concern”. It is endemic to old-growth cedar-hemlock forests in western North America. This is the first study to place N. occultum into a phylogenetic framework using nucleotide sequence and secondary structure data. It also addresses the phylogenetic relationship between N. occultum and N. arcticum. Analysis of fungal Internal Transcribed Spacer (ITS) ribosomal DNA (rDNA) produced four major clades. The cyanobacterial transfer RNALeu intron (tRNALeu) from N. occultum was separated from that of N. isidiosum and fell between the Eurasian and North American epiphytic taxa. Examination of length and complexity of the folded secondary structures revealed different trends in the ITS1 and ITS2 rRNA regions. Even though N. occultum is endemic to North America, it seems more closely related to South American temperate rainforest species than to the sympatric N. arcticum. Nephroma occultum is alone among the studied species of Peltigerales in having an exceptionally long ITS1 region, and a different tRNALeu intron DNA sequence of the photobiont suggesting association with a unique genotype of Nostoc. It may be argued that the fitness of N. occultum may be influenced by the complex ITS1 RNA structure, a unique photobiont genotype undergoing a genetic bottleneck, no sexual reproduction to generate variation, and the inability to associate with different photobionts to adapt to changing habitats.

Research Article
© British Lichen Society 2006

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