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The description of a new species reveals underestimated diversity in the lichen genus Bulbothrix (Parmeliaceae) in Africa

  • Didier Masson (a1), Michel N. Benatti (a2) and Emmanuël Sérusiaux (a3)


Bulbothrix johannis is described as new to science. The species is morphologically similar to the South American Bulbothrix pseudofungicola Benatti & Marcelli but has wider lobes, laminal ciliate isidia which frequently develop into phyllidia, larger cilia and rhizines, and longer ascospores. This epiphytic species occurs fairly frequently in the cloud forests and montane thickets on Réunion Island (Mascarene archipelago). The comparison of B. johannis with morphologically and chemically similar species shows that several different African taxa are currently grouped under the widely used but problematic name B. suffixa (Stirt.) Hale.



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Alstrup, V., Aptroot, A., Divakar, P. K., LaGreca, S. & Tibell, L. (2010) Lichens from Tanzania and Kenya III. Macrolichens and calicioid lichens. Cryptogamie, Mycologie 31: 333351.
Aptroot, A. (1990) Lichens of Madagascar: new and interesting records and species. Cryptogamie, Bryologie et Lichénologie 11: 401408.
Benatti, M. N. (2010) Revisão taxonômica do gênero Bulbothrix Hale (Parmeliaceae, Ascomycota liquenizados). Ph.D. thesis, Instituto de Botânica, São Paulo.
Benatti, M. N. (2011) Two new species of Bulbothrix Hale. Mycology 2: 255259.
Benatti, M. N. (2012 a) Three resurrected species of the genus Bulbothrix Hale (Parmeliaceae, lichenized Fungi). Mycosphere 3: 4655.
Benatti, M. N. (2012 b) A review of the genus Bulbothrix Hale: the species with medullary norstictic or protocetraric acids. MycoKeys 2: 128.
Benatti, M. N. (2012 c) New species of Bulbothrix Hale containing gyrophoric acid from Brazil. Mycology 3: 127131.
Benatti, M. N. (2012 d) A review of the genus Bulbothrix Hale: the species with medullary salazinic acid lacking vegetative propagules. MycoKeys 5: 130.
Benatti, M. N. (2013 a) A review of the genus Bulbothrix Hale: the isidiate, sorediate, and pustulate species with medullary salazinic acid. Mycosphere 4: 130.
Benatti, M. N. (2013 b) A review of the genus Bulbothrix Hale: the species with medullary fatty acids or without medullary substances. Mycosphere 4: 303331.
Benatti, M. N. (2013 c) A review of the genus Bulbothrix Hale: species with gyrophoric, lecanoric or lobaric acids lacking vegetative propagules. Opuscula Philolichenum 12: 151173.
Benatti, M. N. (2014) A review of the genus Bulbothrix Hale: the isidiate, lacinulate, sorediate and pustulate species with medullary gyrophoric, lecanoric and lobaric acids, together with a world key for the genus. Opuscula Philolichenum 13: 122154.
Benatti, M. N. & Elix, J. A. (2012) The true identity of Bulbothrix goebelii (Zenker) Hale and the re-establishment of some of its synonyms as accepted species. Lichenologist 44: 813826.
Bungartz, F., Benatti, M. N. & Spielmann, A. A. (2013) The genus Bulbothrix (Parmeliaceae, Lecanoromycetes) in the Galapagos Islands: a case study of superficially similar, but overlooked macrolichens. Bryologist 116: 358372.
Bussmann, R. W. (2006) Vegetation zonation and nomenclature of African Mountains – an overview. Lyonia 11: 4166.
Calvelo, S. & Adler, M. T. (1999) Parmelia araucana sp. nov. and new reports in the Parmeliaceae sensu stricto (lichenized Ascomycotina) from Patagonia and Tierra del Fuego (Argentina). Sydowia 51: 145154.
Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540552.
Crespo, A., Kauff, F., Divakar, P. K., del Prado, R., Pérez-Ortega, S., Amo de Paz, G., Ferencova, Z., Blanco, O., Roca-Valiente, B., Núñez-Zapata, J. et al. (2010) Phylogenetic generic classification of parmelioid lichens (Parmeliaceae, Ascomycota) based on molecular, morphological and chemical evidence. Taxon 59: 17351753.
Cubero, O. F., Crespo, A., Fatehi, J. & Bridge, P. D. (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Systematics and Evolution 216: 243249.
Divakar, P. K., Crespo, A., Blanco, O. & Lumbsch, H. T. (2006) Phylogenetic significance of morphological characters in the tropical Hypotrachyna clade of parmelioid lichens (Parmeliaceae, Ascomycota). Molecular Phylogenetics and Evolution 40: 448458.
Divakar, P. K., Lumbsch, H. T., Ferencova, Z., del Prado, R. & Crespo, A. (2010) Remototrachyna, a newly recognized tropical lineage of lichens in the Hypotrachyna clade (Parmeliaceae, Ascomycota), originated in the Indian subcontinent. American Journal of Botany 97: 579590.
Elix, J. A. (1993) Progress in the generic delimitation of Parmelia sensu lato lichens (Ascomycotina: Parmeliaceae) and a synoptic key to the Parmeliaceae . Bryologist 96: 359383.
Elix, J. A. (2014) A Catalogue of Standardized Chromatographic Data and Biosynthetic Relationships for Lichen Substances. Third Edition. Canberra: Published by the author.
Ferencova, Z. (2012) Estudio morfológico comparado de los caracteres generativos en relación con linajes monofiléticos de la familia Parmeliaceae (Lecanorales, Ascomycota). Ph.D. thesis, Universidad Complutense de Madrid.
Gardes, M. & Bruns, T. D. (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113118.
Hale, M. E. (1974) Bulbothrix, Parmelina, Relicina, and Xanthoparmelia, four new genera in the Parmeliaceae (Lichenes). Phytologia 28: 479490.
Hale, M. E. (1976) A monograph of the lichen genus Bulbothrix (Parmeliaceae). Smithsonian Contributions to Botany 32: 129.
Hale, M. E. & Kurokawa, S. (1964) Studies on Parmelia subgenus Parmelia. Contributions from the United States National Herbarium 36: 121191.
Jumaux, G., Quetelard, H. & Roy, D. (2011) Atlas Climatique de La Réunion. Météo-France.
Katoh, K. & Standley, D. M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772780.
Maddison, D. R. & Maddison, W. P. (2005) MacClade v. 4.08. Sunderland, Massachusetts: Sinauer Assocociates.
Masson, D. (2012) Hypotrachyna altorum sp. nov., a new lichen from the cloud forests of Réunion Island, Indian Ocean. Cryptogamie, Mycologie 33: 203212.
Miller, M. A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetics trees. In Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, Louisiana, pp. 18.
Mittermeier, R. A., Gil, P. R., Hoffman, M., Pilgrim, J., Brooks, T., Mittermeier, C. G., Lamoreux, J. & da Fonseca, G. A. B. (2004) Hotspots Revisited: Earth’s Biologically Richest and Most Endangered Terrestrial Ecoregions. Mexico: CEMEX.
Olson, D. M. & Dinerstein, E. (2002) The Global 200: priority ecoregions for global conservation. Annals of the Missouri Botanical Garden 89: 199224.
Orange, A., James, P. W. & White, F. J. (2001) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.
Orock, E. A., Leavitt, S. D., Fonge, B. A., St. Clair, L. L. & Lumbsch, H. T. (2012) DNA-based identification of lichen-forming fungi: can publicly available sequence databases aid in lichen diversity inventories of Mount Cameroon (West Africa)? Lichenologist 44: 833839.
Rambaut, A. (2009) FigTree v1.2.3. Available at
Rivas-Martínez, S. & Rivas-Sáenz, S. (2009) Worldwide Bioclimatic Classification System. Phytosociological Research Center, Madrid.
Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 26882690.
Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML Web servers. Systematic Biology 57: 758771.
Stirton, J. (1878) On certain lichens belonging to the genus Parmelia . Scottish Naturalist 4: 200–203, 252–254, 298299.
Strasberg, D., Rouget, M., Richardson, D. M., Baret, S., Dupont, J. & Cowling, R. M. (2005) An assessment of habitat diversity and transformation on La Réunion Island (Mascarene Islands, Indian Ocean) as a basis for identifying broad-scale conservation priorities. Biodiversity and Conservation 14: 30153032.
Swinscow, T. D. V. & Krog, H. (1988) Macrolichens of East Africa. London: British Museum (Natural History).
van den Boom, P. P. G., Brand, M., Ertz, D., Kalb, K., Magain, N., Masson, D., Schiefelbein, U., Sipman, H. J. M. & Sérusiaux, E. (2011) Discovering the lichen diversity of a remote tropical island: working list of species collected on Reunion (Mascarene archipelago, Indian Ocean). Herzogia 24: 325349.
White, T. J., Bruns, T., Lee, S. & Taylor, J. W , . (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications (M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White, eds): 315322. San Diego: Academic Press.
Zhang, Y. Y., Wang, X. Y., Liu, D., Li, J. W., Shi, H. X., Ye, X. & Wang, L. S. (2014) Bulbothrix asiatica sp. nov., and other new records of Parmeliaceae with bulbate cilia from Cambodia. Bryologist 117: 379385.
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31: 511516.
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