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Response of neuroglia to hypoxia-induced oxidative stress using enzymatically crosslinked hydrogels

Published online by Cambridge University Press:  18 December 2019

Samantha G. Zambuto
Affiliation:
Department of Bioengineering, University of Illinois at Urbana-Champaign, 1406 W. Green St, Urbana, IL61801, USA
Julio F. Serrano
Affiliation:
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA
Avery C. Vilbert
Affiliation:
Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA99354, USA
Yi Lu
Affiliation:
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA Pacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA99354, USA Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL61801, USA
Brendan A.C. Harley
Affiliation:
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA
Sara Pedron*
Affiliation:
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 1206 West Gregory Drive, Urbana, IL61801, USA
*
Address all correspondence to Sara Pedron at spedron@illinois.edu
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Abstract

Three-dimensional cultures have exciting potential to mimic aspects of healthy and diseased brain tissue to examine the role of physiological conditions on neural biomarkers, as well as disease onset and progression. Hypoxia is associated with oxidative stress, mitochondrial damage, and inflammation, key processes potentially involved in Alzheimer's and multiple sclerosis. We describe the use of an enzymatically-crosslinkable gelatin hydrogel system within a microfluidic device to explore the effects of hypoxia-induced oxidative stress on rat neuroglia, human astrocyte reactivity, and myelin production. This versatile platform offers new possibilities for drug discovery and modeling disease progression.

Type
Research Letters
Copyright
Copyright © Materials Research Society 2019

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Footnotes

These authors contributed equally to this work.

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